CFSC-8B鼠肝(gan)星形細胞, 進口胎牛(niu)血(xue)清慧穎生物一(yi)站式采購商(shang)城，細(xi)胞株(zhu)細(xi)胞系，耐藥(yao)株(zhu)，STR鑒定，各品牌進口胎牛血清，培(pei)養基，胰酶(mei)，雙抗，*培(pei)養基，PBS緩(huan)沖液，DMSO，細(xi)胞生長(chang)因子等，種(zhong)類齊全，*！

細(xi)(xi)胞培(pei)(pei)養技(ji)(ji)術(shu)屬于細(xi)(xi)胞工(gong)(gong)程范疇，細(xi)(xi)胞工(gong)(gong)程包(bao)括細(xi)(xi)胞融合技(ji)(ji)術(shu)、單克隆抗體(ti)的(de)制備(bei)、核移植技(ji)(ji)術(shu)（克隆）等,而這(zhe)些技(ji)(ji)術(shu)中都有細(xi)(xi)胞培(pei)(pei)養過程,所以說細(xi)(xi)胞培(pei)(pei)養是生物(wu)學(xue)研(yan)究的(de)zui基本(ben)技(ji)(ji)術(shu)之一。

上海慧穎生物供應CFSC-8B鼠(shu)肝星形(xing)細(xi)胞, 進口胎牛血清，活性好、存活率高。隨(sui)著細(xi)胞生物學尤其是干細(xi)胞研究的快速(su)發展，在(zai)細(xi)胞治療領(ling)域已(yi)經(jing)進行了大量的探索，取得了一系(xi)列(lie)的令人振奮的成果(guo)，且部(bu)分基(ji)礎研究成果(guo)已(yi)經(jing)在(zai)心血(xue)管系(xi)統疾(ji)(ji)病(bing) 、神(shen)經(jing)系(xi)統疾(ji)(ji)病(bing)、肌肉骨骼相關疾(ji)(ji)病(bing)、糖尿病(bing)等。

養細(xi)胞(bao)經驗分享：

1.細(xi)(xi)(xi)胞形(xing)態觀察。細(xi)(xi)(xi)胞膜是(shi)每(mei)天必須觀察的(de)(de)，看是(shi)否清晰，細(xi)(xi)(xi)胞的(de)(de)死亡往往是(shi)從細(xi)(xi)(xi)胞膜的(de)(de)破裂開(kai)始的(de)(de)，早期的(de)(de)表(biao)現就(jiu)是(shi)細(xi)(xi)(xi)胞膜某一點出現欠完整或者毛發影。

2.細胞(bao)往(wang)往(wang)會有“抱(bao)(bao)(bao)團”現象(xiang)，抱(bao)(bao)(bao)團生長或死亡，個人認為應該勤換液或傳代，盡量避(bi) 免細胞(bao)抱(bao)(bao)(bao)團。

3.細(xi)(xi)胞生長(chang)迅速，強烈建議用較大(da)的(de)(de)培養(yang)(yang)瓶(ping)養(yang)(yang)，用小瓶(ping)養(yang)(yang)的(de)(de)話，換液不及時，很(hen)容易死 亡。細(xi)(xi)胞的(de)(de)生長(chang)速度一般為2天左右就需要(yao)傳(chuan)代，并(bing)且一般為一傳(chuan)三，或一傳(chuan)四。

4.培(pei)養液：1640|DMEM|MEM|IMDM|a-MEM培(pei)養液，1L一(yi)般加碳(tan)酸氫(qing)鈉2g，并(bing)加雙抗(kang)，胎牛血(xue)清濃度*為(wei)12%，zui低(di)不低(di)于10%，有些細(xi)胞對胎牛血(xue)清高(gao)度依賴，比(bi)如(ru)WRO細(xi)胞，HT-22細(xi)胞，HA-VSMC細(xi)胞，NK-92細(xi)胞等。培養液PH應調為7.20-7.40之(zhi)間。

5.防污染(ran)。L-02細胞生長迅速，較少發(fa)生污染(ran)，但常規措施(shi)應該做(zuo)好(hao)。比如無菌操作，酒精 消毒(du)等，培養瓶口建議過火(huo)，包括瓶蓋，應過火(huo)4秒左(zuo)右。

6.凍存。-20一小時，-80次(ci)夜，液氮長(chang)期保存。強(qiang)烈建(jian)議液氮長(chang)期保存，盡量不要(yao)在-80長(chang)期 保存，死亡(wang)率很高。

7.復蘇(su)。調節(jie)水浴(yu)箱溫(wen)度(du)為42℃，并提前(qian)在(zai)試管(guan)內放置10倍培養(yang)(yang)液，使培養(yang)(yang)液溫(wen)度(du)與室溫(wen)一 致。1分(fen)鐘內融(rong)化細胞(禁(jin)止搖晃凍存管(guan))，移(yi)取至試管(guan)內，動作要輕，從試管(guan)管(guan)底開始慢慢上提 移(yi)液管(guan)，使細胞在(zai)培養(yang)(yang)液內分(fen)布(bu)均(jun)勻，離心，洗(xi)2次(ci)。

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其它經驗概括：

細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)(lei)型(xing)(xing)(xing)(xing)(xing) 編輯(ji) 細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)(lei)型(xing)(xing)(xing)(xing)(xing)根據是(shi)否附于支(zhi)(zhi)持(chi)物(wu)上(shang)(shang)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)特性(xing)，分為(wei) 貼(tie)(tie)附型(xing)(xing)(xing)(xing)(xing)、懸(xuan)(xuan)浮型(xing)(xing)(xing)(xing)(xing)。 貼(tie)(tie)附型(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附在(zai)支(zhi)(zhi)持(chi)物(wu)表(biao)面生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)，只依賴貼(tie)(tie)附才能生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)(tie)附型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這(zhe)種現象(xiang)與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分化(hua)有關(guan)。 貼(tie)(tie)附型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)分型(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自(zi)中(zhong)胚(pei)層 特點(dian)(dian)：與(yu)體(ti)內(nei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)態(tai)(tai)相似(si),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭(suo)型(xing)(xing)(xing)(xing)(xing)或(huo)不規則三角(jiao)形(xing)(xing)(xing),中(zhong)央有圓(yuan)形(xing)(xing)(xing)核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向(xiang)外(wai)伸出2～3個(ge)長(chang)(chang)(chang)(chang)短不同(tong)的(de)(de)(de)(de)突起。細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)時(shi)(shi)呈(cheng)放射(she)狀,漩(xuan)渦或(huo)火焰狀走(zou)行。 起源(yuan)(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自(zi)中(zhong)胚(pei)層間(jian)(jian)充質(zhi)組(zu)(zu)織。 除真正(zheng)的(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平(ping)滑肌(ji)、成(cheng)(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管內(nei)皮(pi)(pi)(pi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意(yi)：細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培(pei)養(yang)時(shi)(shi)成(cheng)(cheng)(cheng)(cheng)為(wei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是(shi)一(yi)(yi)種慣稱,與(yu)體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不同(tong)。 2.上(shang)(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自(zi)外(wai)胚(pei)層。 特點(dian)(dian)：扁平(ping)不規則多(duo)(duo)角(jiao)型(xing)(xing)(xing)(xing)(xing)、圓(yuan)形(xing)(xing)(xing)核、細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相連(lian)成(cheng)(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)殖(zhi)數目增(zeng)多(duo)(duo)時(shi)(shi),整(zheng)個(ge)上(shang)(shang)皮(pi)(pi)(pi)膜隨(sui)之移動(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很(hen)少脫離細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活(huo)動(dong)“ 拉網”現象(xiang)與(yu)起源(yuan)(yuan)內(nei)外(wai)胚(pei)層組(zu)(zu)織有關(guan)。 組(zu)(zu)織: 皮(pi)(pi)(pi)膚表(biao)皮(pi)(pi)(pi)及其衍生(sheng)(sheng)(sheng)物(wu)(汗腺、皮(pi)(pi)(pi)脂腺)消化(hua)道分上(shang)(shang)皮(pi)(pi)(pi)、肝、胰(yi)、肺泡上(shang)(shang)皮(pi)(pi)(pi)。 3. 游走(zou)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點(dian)(dian)：在(zai)支(zhi)(zhi)持(chi)物(wu)上(shang)(shang)散(san)在(zai)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang),不連(lian)接(jie)成(cheng)(cheng)(cheng)(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸出偽(wei)足(zu)或(huo)突起呈(cheng)活(huo)躍的(de)(de)(de)(de)游走(zou)或(huo)變(bian)形(xing)(xing)(xing)運動(dong),速(su)度(du)快不規則, 密(mi)度(du)大連(lian)接(jie)成(cheng)(cheng)(cheng)(cheng)片(pian)呈(cheng)多(duo)(duo)角(jiao)形(xing)(xing)(xing)不易與(yu)其他類(lei)(lei)型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別。 4. 多(duo)(duo)形(xing)(xing)(xing)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點(dian)(dian)： 無規律的(de)(de)(de)(de)形(xing)(xing)(xing)態(tai)(tai)，如(ru)神經(jing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸(xuan)(xuan)浮型(xing)(xing)(xing)(xing)(xing) 特點(dian)(dian)： 不貼(tie)(tie)附在(zai)支(zhi)(zhi)持(chi)物(wu)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓(yuan)形(xing)(xing)(xing)，在(zai)培(pei)養(yang)液中(zhong)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)空(kong)間(jian)(jian)大，可(ke)(ke)長(chang)(chang)(chang)(chang)時(shi)(shi)間(jian)(jian)的(de)(de)(de)(de)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)，繁殖(zhi)旺(wang)盛便(bian)(bian)于做細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉(rou)瘤、血液里的(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)目的(de)(de)(de)(de)： 方便(bian)(bian)描述(shu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培(pei)養(yang)過程中(zhong)的(de)(de)(de)(de)形(xing)(xing)(xing)態(tai)(tai)變(bian)化(hua)。培(pei)養(yang)條件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩定，可(ke)(ke)反(fan)映出起源(yuan)(yuan)、正(zheng)常異(yi)常的(de)(de)(de)(de)區(qu)別，作(zuo)為(wei)判定細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)物(wu)學性(xing)狀指標(biao)。 強(qiang)調(diao)：一(yi)(yi)般形(xing)(xing)(xing)態(tai)(tai)并不是(shi)一(yi)(yi)項可(ke)(ke)靠指標(biao)要(yao)受各方面影響(xiang)。 如(ru)：反(fan)復開(kai)關(guan)溫(wen)箱、溫(wen)度(du)、C02的(de)(de)(de)(de)濃度(du)、培(pei)養(yang)基(ji)變(bian)堿、支(zhi)(zhi)原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)接(jie)種時(shi)(shi)呈(cheng)三角(jiao)型(xing)(xing)(xing)(xing)(xing)狀態(tai)(tai)，經(jing)培(pei)養(yang)一(yi)(yi)定時(shi)(shi)間(jian)(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)傳代前已(yi)形(xing)(xing)(xing)成(cheng)(cheng)(cheng)(cheng)上(shang)(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

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