RLE-6TN鼠(shu)肺上皮Ⅱ型細胞(bao),進口胎牛血清培養慧穎生物一站式采(cai)購商城，細(xi)胞株細(xi)胞系，耐藥株，STR鑒定(ding)，各品牌(pai)進口胎牛血(xue)清(qing)，培養(yang)基，胰(yi)酶，雙(shuang)抗，*培養(yang)基，PBS緩沖液(ye)，DMSO，細(xi)胞生長因子等，種(zhong)類齊全，*！

細胞培(pei)養(yang)(yang)技術屬于細胞工程(cheng)(cheng)范疇，細胞工程(cheng)(cheng)包括(kuo)細胞融合技術、單克(ke)隆抗體的制備、核(he)移植(zhi)技術（克(ke)隆）等,而(er)這些技術中(zhong)都有細胞培(pei)養(yang)(yang)過(guo)程(cheng)(cheng),所以說細胞培(pei)養(yang)(yang)是生物學研(yan)究的zui基本技術之一(yi)。

上海慧穎生物供(gong)應RLE-6TN鼠(shu)肺(fei)上皮Ⅱ型細胞,進口(kou)胎(tai)牛血清(qing)培(pei)養(yang)，活(huo)性(xing)好(hao)、存活(huo)率高(gao)。隨著細(xi)胞生物(wu)學尤其是干細(xi)胞研究(jiu)的(de)快速發展，在(zai)細(xi)胞治療(liao)領域已經進行(xing)了大量(liang)的(de)探(tan)索，取得了一系列的(de)令人振(zhen)奮(fen)的(de)成(cheng)果，且部分基礎(chu)研究(jiu)成(cheng)果已經在(zai)心血管系統疾(ji)(ji)病(bing) 、神經系統疾(ji)(ji)病(bing)、肌肉(rou)骨骼相關疾(ji)(ji)病(bing)、糖尿病(bing)等。

養細胞經驗(yan)分享(xiang)：

1.細(xi)胞(bao)形態觀察。細(xi)胞(bao)膜是(shi)每(mei)天必(bi)須觀察的(de)，看是(shi)否清晰，細(xi)胞(bao)的(de)死(si)亡(wang)往(wang)往(wang)是(shi)從細(xi)胞(bao)膜的(de)破裂開始的(de)，早期的(de)表現(xian)就(jiu)是(shi)細(xi)胞(bao)膜某一點出現(xian)欠完整(zheng)或者(zhe)毛發(fa)影(ying)。

2.細胞往往會有(you)“抱(bao)(bao)團”現象，抱(bao)(bao)團生長(chang)或死亡，個人認為(wei)應該勤換液或傳代，盡量避 免細胞抱(bao)(bao)團。

3.細(xi)胞生(sheng)長迅(xun)速，強烈(lie)建議(yi)用(yong)較大的培養瓶養，用(yong)小(xiao)瓶養的話，換(huan)液不及時，很容易死 亡(wang)。細(xi)胞的生(sheng)長速度一般為(wei)2天左右就需(xu)要傳(chuan)代，并且(qie)一般為(wei)一傳(chuan)三，或(huo)一傳(chuan)四。

4.培養(yang)液：1640|DMEM|MEM|IMDM|a-MEM培養(yang)液，1L一般加碳酸氫鈉2g，并(bing)加雙抗，胎(tai)牛血清濃(nong)度(du)*為12%，zui低不低于(yu)10%，有(you)些(xie)細胞(bao)對胎(tai)牛血清高度(du)依(yi)賴(lai)，比(bi)如WRO細胞(bao)，HT-22細胞(bao)，HA-VSMC細(xi)胞，NK-92細(xi)胞等。培(pei)養液PH應調為7.20-7.40之間。

5.防污染(ran)。L-02細胞生長迅(xun)速(su)，較少發生污染(ran)，但常規措施應該做好。比(bi)如(ru)無菌(jun)操作，酒(jiu)精 消毒等(deng)，培養瓶口(kou)建議(yi)過火，包(bao)括(kuo)瓶蓋(gai)，應過火4秒左右(you)。

6.凍存。-20一小時(shi)，-80次夜(ye)，液氮長(chang)期保存。強烈建議(yi)液氮長(chang)期保存，盡量不要在-80長(chang)期 保存，死(si)亡率很(hen)高。

7.復蘇。調節水(shui)浴箱溫(wen)度為42℃，并提(ti)前在(zai)試(shi)管內(nei)放置(zhi)10倍培養液(ye)，使(shi)培養液(ye)溫(wen)度與(yu)室溫(wen)一 致。1分(fen)鐘(zhong)內(nei)融化細胞(禁止搖晃(huang)凍(dong)存管)，移(yi)取至(zhi)試(shi)管內(nei)，動作要輕，從試(shi)管管底開始慢慢上提(ti) 移(yi)液(ye)管，使(shi)細胞在(zai)培養液(ye)內(nei)分(fen)布均勻，離心，洗(xi)2次(ci)。

相關熱賣(mai)產(chan)品：

其它(ta)經驗概(gai)括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)根據是否附于支(zhi)持(chi)物(wu)上(shang)生(sheng)(sheng)(sheng)長(chang)(chang)的(de)特性(xing)，分(fen)(fen)為 貼(tie)(tie)附型(xing)(xing)(xing)(xing)、懸浮(fu)型(xing)(xing)(xing)(xing)。 貼(tie)(tie)附型(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附在(zai)支(zhi)持(chi)物(wu)表(biao)面生(sheng)(sheng)(sheng)長(chang)(chang)，只依賴貼(tie)(tie)附才能生(sheng)(sheng)(sheng)長(chang)(chang)的(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)(tie)附型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)(fen)化有(you)(you)關(guan)。 貼(tie)(tie)附型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)分(fen)(fen)型(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)纖(xian)維(wei)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自中胚(pei)層(ceng)(ceng)(ceng) 特點：與(yu)體(ti)內(nei)成(cheng)(cheng)(cheng)纖(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)態相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)(xing)或(huo)(huo)不(bu)規(gui)則(ze)三(san)角(jiao)形(xing)(xing)(xing),中央有(you)(you)圓(yuan)(yuan)形(xing)(xing)(xing)核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向外伸(shen)出(chu)2～3個(ge)長(chang)(chang)短不(bu)同(tong)(tong)的(de)突起(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)生(sheng)(sheng)(sheng)長(chang)(chang)時(shi)呈放射狀(zhuang),漩(xuan)渦(wo)或(huo)(huo)火焰狀(zhuang)走行。 起(qi)源(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自中胚(pei)層(ceng)(ceng)(ceng)間充質(zhi)組(zu)織。 除(chu)真正(zheng)的(de)成(cheng)(cheng)(cheng)纖(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌、平滑肌、成(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)(xue)管內(nei)皮(pi)(pi)(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培(pei)(pei)養(yang)時(shi)成(cheng)(cheng)(cheng)為成(cheng)(cheng)(cheng)纖(xian)維(wei)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)種慣稱,與(yu)體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)同(tong)(tong)。 2.上(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自外胚(pei)層(ceng)(ceng)(ceng)。 特點：扁平不(bu)規(gui)則(ze)多(duo)角(jiao)型(xing)(xing)(xing)(xing)、圓(yuan)(yuan)形(xing)(xing)(xing)核、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相連(lian)成(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖(zhi)數目增多(duo)時(shi),整個(ge)上(shang)皮(pi)(pi)(pi)膜隨之移動(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)很(hen)少(shao)脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨(du)活動(dong)“ 拉(la)網”現象與(yu)起(qi)源(yuan)內(nei)外胚(pei)層(ceng)(ceng)(ceng)組(zu)織有(you)(you)關(guan)。 組(zu)織: 皮(pi)(pi)(pi)膚表(biao)皮(pi)(pi)(pi)及其衍生(sheng)(sheng)(sheng)物(wu)(汗(han)腺、皮(pi)(pi)(pi)脂腺)消化道分(fen)(fen)上(shang)皮(pi)(pi)(pi)、肝、胰(yi)、肺(fei)泡上(shang)皮(pi)(pi)(pi)。 3. 游(you)走型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點：在(zai)支(zhi)持(chi)物(wu)上(shang)散在(zai)生(sheng)(sheng)(sheng)長(chang)(chang),不(bu)連(lian)接(jie)(jie)成(cheng)(cheng)(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸(shen)出(chu)偽足或(huo)(huo)突起(qi)呈活躍的(de)游(you)走或(huo)(huo)變(bian)(bian)形(xing)(xing)(xing)運動(dong),速度快不(bu)規(gui)則(ze), 密(mi)度大連(lian)接(jie)(jie)成(cheng)(cheng)(cheng)片呈多(duo)角(jiao)形(xing)(xing)(xing)不(bu)易與(yu)其他(ta)類型(xing)(xing)(xing)(xing)的(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別(bie)。 4. 多(duo)形(xing)(xing)(xing)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點： 無規(gui)律的(de)形(xing)(xing)(xing)態，如神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮(fu)型(xing)(xing)(xing)(xing) 特點： 不(bu)貼(tie)(tie)附在(zai)支(zhi)持(chi)物(wu)生(sheng)(sheng)(sheng)長(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓(yuan)(yuan)形(xing)(xing)(xing)，在(zai)培(pei)(pei)養(yang)液中生(sheng)(sheng)(sheng)長(chang)(chang)空間大，可(ke)長(chang)(chang)時(shi)間的(de)生(sheng)(sheng)(sheng)長(chang)(chang)，繁殖(zhi)旺(wang)盛便于做細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉瘤、血(xue)(xue)液里的(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)(fen)型(xing)(xing)(xing)(xing)的(de)目的(de)： 方便描(miao)述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培(pei)(pei)養(yang)過程中的(de)形(xing)(xing)(xing)態變(bian)(bian)化。培(pei)(pei)養(yang)條(tiao)件好時(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩定，可(ke)反(fan)映出(chu)起(qi)源(yuan)、正(zheng)常異常的(de)區別(bie)，作為判定細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)物(wu)學性(xing)狀(zhuang)指(zhi)標。 強調：一(yi)般形(xing)(xing)(xing)態并不(bu)是一(yi)項可(ke)靠(kao)指(zhi)標要受各方面影響。 如：反(fan)復(fu)開關(guan)溫(wen)(wen)箱、溫(wen)(wen)度、C02的(de)濃度、培(pei)(pei)養(yang)基變(bian)(bian)堿、支(zhi)原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)接(jie)(jie)種時(shi)呈三(san)角(jiao)型(xing)(xing)(xing)(xing)狀(zhuang)態，經培(pei)(pei)養(yang)一(yi)定時(shi)間后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)傳代前已形(xing)(xing)(xing)成(cheng)(cheng)(cheng)上(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細(xi)胞，人(ren)急性骨(gu)髓白血(xue)病細(xi)胞      25T

MV4-11細(xi)胞，人急性白(bai)血(xue)病細(xi)胞    25T

HL-60細胞，人急性早幼粒白血(xue)病細胞    25T

U937細(xi)胞，人急性(xing)早幼粒白血病細(xi)胞     25T

MOLM-13細(xi)胞，人急性髓系白血病細(xi)胞    25T

MOLM-14細(xi)胞，人急性(xing)髓系白血病細(xi)胞    25T

K562細胞，人慢性髓系白血病(bing)細胞  25T

HL-60細胞，人急(ji)性早幼粒白血病細胞    25T

CCRF-CEM細(xi)胞，人(ren)急性淋巴(ba)細(xi)胞白(bai)血病(bing)T淋巴(ba)細(xi)胞  25T

HS 505.T細(xi)胞(bao)，慢性淋巴細(xi)胞(bao)白血病(bing)（CLL）細(xi)胞(bao)   25T