L-929 小(xiao)鼠(shu)成纖維細胞慧穎生物一站式采購商城(cheng)，細(xi)胞株細(xi)胞系，耐藥(yao)株，STR鑒定(ding)，各品牌進口胎牛血清(qing)，培(pei)養基，胰酶，雙抗，*培(pei)養基，PBS緩沖液，DMSO，細(xi)胞生長因子等，種(zhong)類齊全(quan)，*！

細胞(bao)培養(yang)(yang)技(ji)(ji)(ji)術(shu)屬于(yu)細胞(bao)工程(cheng)范(fan)疇，細胞(bao)工程(cheng)包括細胞(bao)融合技(ji)(ji)(ji)術(shu)、單(dan)克隆抗體的制備、核(he)移植技(ji)(ji)(ji)術(shu)（克隆）等(deng),而這些(xie)技(ji)(ji)(ji)術(shu)中都有細胞(bao)培養(yang)(yang)過程(cheng),所以說細胞(bao)培養(yang)(yang)是生物(wu)學研究的zui基本技(ji)(ji)(ji)術(shu)之一(yi)。

上海慧(hui)穎生物供應L-929 小鼠成纖維(wei)細胞，活性好、存活率高(gao)。隨著細(xi)胞(bao)生物學尤其是干(gan)細(xi)胞(bao)研(yan)究的(de)快速發展，在(zai)(zai)細(xi)胞(bao)治療(liao)領域已經(jing)進行了大量的(de)探索，取得(de)了一系(xi)(xi)(xi)列的(de)令人振奮的(de)成果(guo)，且部分基(ji)礎研(yan)究成果(guo)已經(jing)在(zai)(zai)心血管(guan)系(xi)(xi)(xi)統疾(ji)病(bing)(bing) 、神經(jing)系(xi)(xi)(xi)統疾(ji)病(bing)(bing)、肌肉(rou)骨骼相關(guan)疾(ji)病(bing)(bing)、糖尿病(bing)(bing)等。

養細胞經(jing)驗分享：

1.細胞(bao)(bao)形態觀察。細胞(bao)(bao)膜(mo)(mo)是(shi)每天(tian)必須觀察的(de)，看是(shi)否清晰，細胞(bao)(bao)的(de)死亡往往是(shi)從(cong)細胞(bao)(bao)膜(mo)(mo)的(de)破裂開始的(de)，早期的(de)表現就是(shi)細胞(bao)(bao)膜(mo)(mo)某一(yi)點(dian)出現欠完整或者毛發影(ying)。

2.細胞往往會有“抱團”現(xian)象，抱團生(sheng)長(chang)或死(si)亡，個人認為(wei)應該勤(qin)換液或傳代(dai)，盡(jin)量(liang)避 免(mian)細胞抱團。

3.細(xi)胞(bao)生長迅(xun)速(su)，強烈建議用(yong)較大(da)的培養瓶養，用(yong)小(xiao)瓶養的話，換液不及時(shi)，很容易死 亡。細(xi)胞(bao)的生長速(su)度一(yi)般(ban)為(wei)(wei)2天左右就需要傳(chuan)代，并且一(yi)般(ban)為(wei)(wei)一(yi)傳(chuan)三，或一(yi)傳(chuan)四。

4.培養(yang)液(ye)：1640|DMEM|MEM|IMDM|a-MEM培養(yang)液(ye)，1L一般(ban)加碳酸(suan)氫(qing)鈉2g，并加雙抗(kang)，胎(tai)牛血清濃(nong)度*為12%，zui低不低于10%，有些細(xi)(xi)胞(bao)對(dui)胎(tai)牛血清高度依(yi)賴，比如WRO細(xi)(xi)胞(bao)，HT-22細(xi)(xi)胞(bao)，HA-VSMC細胞(bao)，NK-92細胞(bao)等。培(pei)養液(ye)PH應調為7.20-7.40之間。

5.防污染。L-02細胞生(sheng)長迅速，較(jiao)少發生(sheng)污染，但常規(gui)措施應(ying)該做好。比如無菌操作，酒精 消毒等，培養瓶口建議過火(huo)，包括瓶蓋，應(ying)過火(huo)4秒左右。

6.凍存(cun)。-20一小時，-80次夜，液氮長(chang)期(qi)(qi)保(bao)存(cun)。強烈(lie)建議液氮長(chang)期(qi)(qi)保(bao)存(cun)，盡量不要在-80長(chang)期(qi)(qi) 保(bao)存(cun)，死亡(wang)率很高。

7.復(fu)蘇。調節水浴箱(xiang)溫度為42℃，并提前(qian)在試管(guan)(guan)內(nei)放置10倍培(pei)養(yang)液，使培(pei)養(yang)液溫度與室溫一 致(zhi)。1分鐘內(nei)融化細(xi)胞(禁止(zhi)搖晃凍(dong)存管(guan)(guan))，移取至試管(guan)(guan)內(nei)，動(dong)作要輕，從試管(guan)(guan)管(guan)(guan)底(di)開(kai)始慢(man)慢(man)上提 移液管(guan)(guan)，使細(xi)胞在培(pei)養(yang)液內(nei)分布(bu)均(jun)勻，離(li)心(xin)，洗2次。

相關熱(re)賣(mai)產品：

其它(ta)經驗概括(kuo)：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)根據是否附于支(zhi)(zhi)(zhi)持物(wu)上(shang)生(sheng)(sheng)長(chang)的(de)(de)(de)(de)特(te)(te)性，分(fen)(fen)為(wei) 貼(tie)附型(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)。 貼(tie)附型(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)附在(zai)(zai)(zai)支(zhi)(zhi)(zhi)持物(wu)表(biao)面(mian)生(sheng)(sheng)長(chang)，只(zhi)依賴貼(tie)附才(cai)能(neng)生(sheng)(sheng)長(chang)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)附型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象(xiang)與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)分(fen)(fen)化有關(guan)(guan)。 貼(tie)附型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)分(fen)(fen)型(xing)(xing)(xing) 1.成(cheng)纖(xian)維(wei)(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自(zi)中(zhong)胚(pei)層(ceng) 特(te)(te)點(dian)：與(yu)體(ti)內成(cheng)纖(xian)維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)(xing)態(tai)相(xiang)似,胞(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)或不(bu)(bu)(bu)(bu)(bu)規則(ze)(ze)三角形(xing)(xing)(xing)(xing)(xing),中(zhong)央有圓(yuan)形(xing)(xing)(xing)(xing)(xing)核(he)(he),胞(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)向外伸(shen)出(chu)(chu)2～3個(ge)長(chang)短(duan)不(bu)(bu)(bu)(bu)(bu)同的(de)(de)(de)(de)突起(qi)(qi)(qi)(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)生(sheng)(sheng)長(chang)時呈放射狀(zhuang),漩渦或火焰狀(zhuang)走行。 起(qi)(qi)(qi)(qi)源：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自(zi)中(zhong)胚(pei)層(ceng)間充質(zhi)(zhi)組織(zhi)。 除真正的(de)(de)(de)(de)成(cheng)纖(xian)維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、心肌、平(ping)滑肌、成(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)管內皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。 注(zhu)意(yi)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)培(pei)養(yang)時成(cheng)為(wei)成(cheng)纖(xian)維(wei)(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)種慣稱(cheng),與(yu)體(ti)內細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)(bu)同。 2.上(shang)皮(pi)(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自(zi)外胚(pei)層(ceng)。 特(te)(te)點(dian)：扁平(ping)不(bu)(bu)(bu)(bu)(bu)規則(ze)(ze)多角型(xing)(xing)(xing)、圓(yuan)形(xing)(xing)(xing)(xing)(xing)核(he)(he)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)緊(jin)密(mi)(mi)相(xiang)連(lian)成(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)殖數目增(zeng)多時,整個(ge)上(shang)皮(pi)(pi)膜隨之移動(dong)(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)群(qun)而單獨活動(dong)(dong)“ 拉網”現象(xiang)與(yu)起(qi)(qi)(qi)(qi)源內外胚(pei)層(ceng)組織(zhi)有關(guan)(guan)。 組織(zhi): 皮(pi)(pi)膚(fu)表(biao)皮(pi)(pi)及其(qi)衍(yan)生(sheng)(sheng)物(wu)(汗腺(xian)、皮(pi)(pi)脂腺(xian))消(xiao)化道分(fen)(fen)上(shang)皮(pi)(pi)、肝、胰、肺泡上(shang)皮(pi)(pi)。 3. 游走型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)(te)點(dian)：在(zai)(zai)(zai)支(zhi)(zhi)(zhi)持物(wu)上(shang)散(san)在(zai)(zai)(zai)生(sheng)(sheng)長(chang),不(bu)(bu)(bu)(bu)(bu)連(lian)接成(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)伸(shen)出(chu)(chu)偽足或突起(qi)(qi)(qi)(qi)呈活躍(yue)的(de)(de)(de)(de)游走或變形(xing)(xing)(xing)(xing)(xing)運動(dong)(dong),速度(du)快不(bu)(bu)(bu)(bu)(bu)規則(ze)(ze), 密(mi)(mi)度(du)大連(lian)接成(cheng)片呈多角形(xing)(xing)(xing)(xing)(xing)不(bu)(bu)(bu)(bu)(bu)易(yi)與(yu)其(qi)他(ta)類(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別。 4. 多形(xing)(xing)(xing)(xing)(xing)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)(te)點(dian)： 無規律(lv)的(de)(de)(de)(de)形(xing)(xing)(xing)(xing)(xing)態(tai)，如神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing) 特(te)(te)點(dian)： 不(bu)(bu)(bu)(bu)(bu)貼(tie)附在(zai)(zai)(zai)支(zhi)(zhi)(zhi)持物(wu)生(sheng)(sheng)長(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓(yuan)形(xing)(xing)(xing)(xing)(xing)，在(zai)(zai)(zai)培(pei)養(yang)液中(zhong)生(sheng)(sheng)長(chang)空(kong)間大，可長(chang)時間的(de)(de)(de)(de)生(sheng)(sheng)長(chang)，繁殖旺盛便(bian)于做細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉瘤、血(xue)液里的(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)(fen)型(xing)(xing)(xing)的(de)(de)(de)(de)目的(de)(de)(de)(de)： 方便(bian)描述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)培(pei)養(yang)過程中(zhong)的(de)(de)(de)(de)形(xing)(xing)(xing)(xing)(xing)態(tai)變化。培(pei)養(yang)條件好(hao)時，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)相(xiang)對(dui)穩定(ding)，可反映出(chu)(chu)起(qi)(qi)(qi)(qi)源、正常(chang)異常(chang)的(de)(de)(de)(de)區(qu)別，作為(wei)判定(ding)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)物(wu)學性狀(zhuang)指(zhi)標(biao)。 強(qiang)調：一(yi)般形(xing)(xing)(xing)(xing)(xing)態(tai)并不(bu)(bu)(bu)(bu)(bu)是一(yi)項可靠指(zhi)標(biao)要受各方面(mian)影響。 如：反復開關(guan)(guan)溫(wen)箱(xiang)、溫(wen)度(du)、C02的(de)(de)(de)(de)濃度(du)、培(pei)養(yang)基變堿、支(zhi)(zhi)(zhi)原(yuan)體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)接種時呈三角型(xing)(xing)(xing)狀(zhuang)態(tai)，經培(pei)養(yang)一(yi)定(ding)時間后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)傳代前已形(xing)(xing)(xing)(xing)(xing)成(cheng)上(shang)皮(pi)(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。

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