3T3-L1小鼠(shu)前(qian)脂肪細胞, 進口胎(tai)牛血清培養慧穎生物一站(zhan)式(shi)采購商(shang)城，細(xi)胞株細(xi)胞系(xi)，耐藥株，STR鑒定，各(ge)品牌進(jin)口胎(tai)牛(niu)血清(qing)，培養基，胰酶，雙抗，*培養基，PBS緩(huan)沖液，DMSO，細(xi)胞生長因子等，種類齊全，*！

細(xi)胞(bao)培養(yang)技(ji)(ji)術(shu)(shu)屬于細(xi)胞(bao)工程范疇(chou)，細(xi)胞(bao)工程包括細(xi)胞(bao)融合技(ji)(ji)術(shu)(shu)、單克隆(long)抗體的制備、核移(yi)植(zhi)技(ji)(ji)術(shu)(shu)（克隆(long)）等,而這些技(ji)(ji)術(shu)(shu)中(zhong)都有細(xi)胞(bao)培養(yang)過程,所以說細(xi)胞(bao)培養(yang)是生物(wu)學(xue)研究的zui基(ji)本技(ji)(ji)術(shu)(shu)之一。

上海慧(hui)穎(ying)生物供應(ying)3T3-L1小鼠前脂肪(fang)細胞, 進口胎牛血清培養，活性好、存(cun)活率高。隨(sui)著細(xi)胞(bao)生物學尤其是干細(xi)胞(bao)研究(jiu)的(de)快速發展(zhan)，在(zai)細(xi)胞(bao)治療(liao)領域已經(jing)進行(xing)了(le)大量的(de)探索，取得了(le)一(yi)系(xi)列的(de)令人振奮(fen)的(de)成果，且(qie)部分基(ji)礎研究(jiu)成果已經(jing)在(zai)心(xin)血管系(xi)統疾(ji)病(bing)(bing) 、神(shen)經(jing)系(xi)統疾(ji)病(bing)(bing)、肌肉(rou)骨骼相關疾(ji)病(bing)(bing)、糖(tang)尿病(bing)(bing)等。

養(yang)細胞經(jing)驗分享：

1.細(xi)胞(bao)(bao)(bao)形態觀察(cha)(cha)。細(xi)胞(bao)(bao)(bao)膜(mo)是(shi)每天必須觀察(cha)(cha)的(de)，看是(shi)否清晰，細(xi)胞(bao)(bao)(bao)的(de)死亡往往是(shi)從細(xi)胞(bao)(bao)(bao)膜(mo)的(de)破裂開始的(de)，早期的(de)表現就(jiu)是(shi)細(xi)胞(bao)(bao)(bao)膜(mo)某一點(dian)出現欠完(wan)整(zheng)或(huo)者毛發影。

2.細(xi)胞往往會有“抱團”現(xian)象，抱團生(sheng)長或(huo)死亡，個人(ren)認為應該勤換液或(huo)傳代，盡(jin)量避 免細(xi)胞抱團。

3.細胞(bao)生(sheng)長(chang)迅速，強烈(lie)建議(yi)用(yong)較大的培養瓶(ping)養，用(yong)小瓶(ping)養的話，換液(ye)不及(ji)時，很容易死 亡。細胞(bao)的生(sheng)長(chang)速度一般為2天(tian)左右就(jiu)需要(yao)傳(chuan)(chuan)代，并且一般為一傳(chuan)(chuan)三(san)，或一傳(chuan)(chuan)四。

4.培養(yang)液：1640|DMEM|MEM|IMDM|a-MEM培養(yang)液，1L一般(ban)加(jia)(jia)碳酸氫鈉2g，并加(jia)(jia)雙抗，胎牛(niu)血清濃(nong)度*為12%，zui低(di)不低(di)于10%，有(you)些細(xi)胞(bao)對胎牛(niu)血清高(gao)度依賴(lai)，比如WRO細(xi)胞(bao)，HT-22細(xi)胞(bao)，HA-VSMC細胞(bao)，NK-92細胞(bao)等。培養(yang)液PH應調為7.20-7.40之(zhi)間。

5.防污染。L-02細胞生長迅速，較少發(fa)生污染，但常規措施(shi)應(ying)該(gai)做好。比如無菌(jun)操作，酒精 消毒等(deng)，培(pei)養瓶口(kou)建議過火，包括(kuo)瓶蓋，應(ying)過火4秒左右。

6.凍存(cun)。-20一小時，-80次夜，液(ye)(ye)氮長期保(bao)存(cun)。強烈建(jian)議液(ye)(ye)氮長期保(bao)存(cun)，盡(jin)量(liang)不要在(zai)-80長期 保(bao)存(cun)，死亡(wang)率很高。

7.復蘇。調節水浴箱溫(wen)度(du)為42℃，并提(ti)(ti)前在(zai)試(shi)管(guan)內放置10倍培養液，使培養液溫(wen)度(du)與室溫(wen)一 致。1分鐘內融化(hua)細胞(禁止搖晃凍存管(guan))，移取至試(shi)管(guan)內，動作要輕，從試(shi)管(guan)管(guan)底開始慢(man)慢(man)上(shang)提(ti)(ti) 移液管(guan)，使細胞在(zai)培養液內分布(bu)均勻(yun)，離心(xin)，洗(xi)2次。

相關熱(re)賣產品：

其它經驗(yan)概括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing)根據是否附于支(zhi)持物上(shang)生(sheng)長(chang)的(de)(de)特性，分(fen)為(wei) 貼(tie)附型(xing)(xing)(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)(xing)(xing)。 貼(tie)附型(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)附在支(zhi)持物表(biao)(biao)面生(sheng)長(chang)，只依(yi)賴貼(tie)附才能生(sheng)長(chang)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做(zuo)(zuo)貼(tie)附型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種(zhong)(zhong)(zhong)現象與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化(hua)有(you)關(guan)。 貼(tie)附型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)分(fen)型(xing)(xing)(xing)(xing)(xing) 1.成(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自(zi)(zi)中(zhong)胚(pei)層 特點(dian)：與(yu)體內(nei)(nei)(nei)成(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)態相似(si),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體梭型(xing)(xing)(xing)(xing)(xing)或不(bu)(bu)規則三角(jiao)(jiao)形(xing)(xing)(xing)(xing),中(zhong)央有(you)圓形(xing)(xing)(xing)(xing)核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質向外伸(shen)出2～3個(ge)長(chang)短(duan)不(bu)(bu)同的(de)(de)突起(qi)(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在生(sheng)長(chang)時(shi)(shi)呈(cheng)(cheng)放(fang)射狀,漩渦或火焰狀走行(xing)。 起(qi)(qi)源：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自(zi)(zi)中(zhong)胚(pei)層間(jian)(jian)充質組(zu)(zu)織(zhi)。 除真正的(de)(de)成(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心(xin)肌、平(ping)滑肌、成(cheng)骨(gu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管(guan)內(nei)(nei)(nei)皮(pi)(pi)(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在培養(yang)(yang)時(shi)(shi)成(cheng)為(wei)成(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)種(zhong)(zhong)(zhong)慣稱,與(yu)體內(nei)(nei)(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)同。 2.上(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自(zi)(zi)外胚(pei)層。 特點(dian)：扁平(ping)不(bu)(bu)規則多角(jiao)(jiao)型(xing)(xing)(xing)(xing)(xing)、圓形(xing)(xing)(xing)(xing)核、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相連成(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)殖數目增(zeng)多時(shi)(shi),整個(ge)上(shang)皮(pi)(pi)(pi)膜隨(sui)之移動(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少(shao)脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活動(dong)“ 拉網(wang)”現象與(yu)起(qi)(qi)源內(nei)(nei)(nei)外胚(pei)層組(zu)(zu)織(zhi)有(you)關(guan)。 組(zu)(zu)織(zhi): 皮(pi)(pi)(pi)膚表(biao)(biao)皮(pi)(pi)(pi)及其(qi)衍(yan)生(sheng)物(汗腺(xian)、皮(pi)(pi)(pi)脂腺(xian))消化(hua)道分(fen)上(shang)皮(pi)(pi)(pi)、肝(gan)、胰、肺(fei)泡上(shang)皮(pi)(pi)(pi)。 3. 游走型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點(dian)：在支(zhi)持物上(shang)散在生(sheng)長(chang),不(bu)(bu)連接成(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質伸(shen)出偽足(zu)或突起(qi)(qi)呈(cheng)(cheng)活躍的(de)(de)游走或變(bian)(bian)形(xing)(xing)(xing)(xing)運動(dong),速度快不(bu)(bu)規則, 密(mi)度大連接成(cheng)片呈(cheng)(cheng)多角(jiao)(jiao)形(xing)(xing)(xing)(xing)不(bu)(bu)易與(yu)其(qi)他類型(xing)(xing)(xing)(xing)(xing)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別(bie)。 4. 多形(xing)(xing)(xing)(xing)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點(dian)： 無規律的(de)(de)形(xing)(xing)(xing)(xing)態，如神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing)(xing)(xing) 特點(dian)： 不(bu)(bu)貼(tie)附在支(zhi)持物生(sheng)長(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體圓形(xing)(xing)(xing)(xing)，在培養(yang)(yang)液(ye)中(zhong)生(sheng)長(chang)空(kong)間(jian)(jian)大，可長(chang)時(shi)(shi)間(jian)(jian)的(de)(de)生(sheng)長(chang)，繁殖旺盛便于做(zuo)(zuo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉瘤、血液(ye)里的(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)(xing)的(de)(de)目的(de)(de)： 方便描述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在培養(yang)(yang)過程中(zhong)的(de)(de)形(xing)(xing)(xing)(xing)態變(bian)(bian)化(hua)。培養(yang)(yang)條件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩定(ding)，可反(fan)映出起(qi)(qi)源、正常異常的(de)(de)區(qu)別(bie)，作為(wei)判定(ding)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)物學性狀指(zhi)標(biao)。 強調：一(yi)般形(xing)(xing)(xing)(xing)態并不(bu)(bu)是一(yi)項可靠指(zhi)標(biao)要受(shou)各方面影響。 如：反(fan)復(fu)開(kai)關(guan)溫(wen)箱(xiang)、溫(wen)度、C02的(de)(de)濃度、培養(yang)(yang)基變(bian)(bian)堿、支(zhi)原體、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在接種(zhong)(zhong)(zhong)時(shi)(shi)呈(cheng)(cheng)三角(jiao)(jiao)型(xing)(xing)(xing)(xing)(xing)狀態，經培養(yang)(yang)一(yi)定(ding)時(shi)(shi)間(jian)(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在傳代前(qian)已形(xing)(xing)(xing)(xing)成(cheng)上(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細胞，人(ren)急性骨髓白血(xue)病細胞      25T

MV4-11細(xi)胞(bao)(bao)，人急性白血病細(xi)胞(bao)(bao)    25T

HL-60細(xi)胞，人急性(xing)早幼(you)粒白血病細(xi)胞    25T

U937細胞(bao)，人急性早幼粒(li)白(bai)血病(bing)細胞(bao)     25T

MOLM-13細(xi)胞，人急性髓(sui)系白血病細(xi)胞    25T

MOLM-14細(xi)胞(bao)，人急(ji)性(xing)髓系(xi)白血(xue)病細(xi)胞(bao)    25T

K562細胞，人(ren)慢(man)性髓系白(bai)血(xue)病細胞  25T

HL-60細胞，人急性(xing)早幼粒白(bai)血病細胞    25T

CCRF-CEM細胞，人急(ji)性淋巴(ba)細胞白血病T淋巴(ba)細胞  25T

HS 505.T細(xi)胞(bao)，慢性淋(lin)巴細(xi)胞(bao)白(bai)血病（CLL）細(xi)胞(bao)   25T