HA-VSMC人升主動脈平滑(hua)肌細(xi)胞(bao)，進口胎牛血清培養(yang)慧穎(ying)生(sheng)物一站式采購(gou)商城，細胞(bao)株細胞(bao)系(xi)，耐藥株，STR鑒定，各(ge)品(pin)牌進(jin)口胎牛血清，培(pei)養基，胰(yi)酶，雙抗，*培(pei)養基，PBS緩(huan)沖液，DMSO，細胞(bao)生(sheng)長因子(zi)等，種類(lei)齊全(quan)，*！

細胞培(pei)養技(ji)術(shu)(shu)屬于(yu)細胞工程(cheng)范疇，細胞工程(cheng)包括細胞融合技(ji)術(shu)(shu)、單克隆(long)抗體的制備、核(he)移植技(ji)術(shu)(shu)（克隆(long)）等,而這些技(ji)術(shu)(shu)中都(dou)有細胞培(pei)養過程(cheng),所以說(shuo)細胞培(pei)養是生物(wu)學研(yan)究的zui基本(ben)技(ji)術(shu)(shu)之(zhi)一。

上海慧穎生物供應HA-VSMC人升主(zhu)動脈平滑肌(ji)細胞(bao)，進口胎(tai)牛血(xue)清培養，活(huo)性好、存(cun)活(huo)率(lv)高(gao)。隨著細(xi)胞(bao)生物學尤其是干細(xi)胞(bao)研究的快速(su)發(fa)展，在細(xi)胞(bao)治療領域已(yi)經(jing)(jing)進行了大量的探索(suo)，取得了一系(xi)列的令人振(zhen)奮的成(cheng)果，且部分基(ji)礎研究成(cheng)果已(yi)經(jing)(jing)在心血管系(xi)統疾病(bing) 、神經(jing)(jing)系(xi)統疾病(bing)、肌肉骨骼相關疾病(bing)、糖尿病(bing)等。

養細胞經驗(yan)分享(xiang)：

1.細(xi)胞(bao)形態觀察(cha)。細(xi)胞(bao)膜是(shi)(shi)每天必須(xu)觀察(cha)的，看是(shi)(shi)否清晰，細(xi)胞(bao)的死亡(wang)往往是(shi)(shi)從(cong)細(xi)胞(bao)膜的破裂(lie)開(kai)始的，早期(qi)的表現(xian)就(jiu)是(shi)(shi)細(xi)胞(bao)膜某一點出現(xian)欠完整或者(zhe)毛發影。

2.細(xi)胞(bao)往(wang)往(wang)會有(you)“抱團(tuan)”現象，抱團(tuan)生(sheng)長或死亡，個人認為(wei)應該勤換液或傳代，盡量避 免細(xi)胞(bao)抱團(tuan)。

3.細胞生(sheng)(sheng)長迅速，強烈建議(yi)用(yong)較大的(de)培(pei)養瓶(ping)養，用(yong)小瓶(ping)養的(de)話(hua)，換液不及時，很容易死(si) 亡(wang)。細胞的(de)生(sheng)(sheng)長速度一(yi)(yi)般(ban)為2天(tian)左右就需要(yao)傳(chuan)(chuan)代，并且一(yi)(yi)般(ban)為一(yi)(yi)傳(chuan)(chuan)三，或一(yi)(yi)傳(chuan)(chuan)四(si)。

4.培(pei)養(yang)(yang)液(ye)：1640|DMEM|MEM|IMDM|a-MEM培(pei)養(yang)(yang)液(ye)，1L一般(ban)加(jia)碳酸氫(qing)鈉2g，并加(jia)雙(shuang)抗，胎(tai)(tai)牛(niu)血清濃(nong)度*為12%，zui低不低于10%，有(you)些(xie)細胞(bao)對胎(tai)(tai)牛(niu)血清高度依(yi)賴(lai)，比如WRO細胞(bao)，HT-22細胞(bao)，HA-VSMC細胞(bao)，NK-92細胞(bao)等。培養液PH應調為7.20-7.40之間。

5.防(fang)污染(ran)。L-02細胞生(sheng)長(chang)迅(xun)速，較少發生(sheng)污染(ran)，但常(chang)規措施應該做好。比如無菌(jun)操作，酒精 消毒(du)等，培養瓶口建(jian)議(yi)過火，包括瓶蓋，應過火4秒左右。

6.凍存(cun)(cun)。-20一小時，-80次夜，液氮(dan)長(chang)期(qi)保存(cun)(cun)。強(qiang)烈建議(yi)液氮(dan)長(chang)期(qi)保存(cun)(cun)，盡(jin)量不要在-80長(chang)期(qi) 保存(cun)(cun)，死亡率(lv)很高(gao)。

7.復蘇(su)。調節水浴箱溫(wen)度為(wei)42℃，并提(ti)前在試管內(nei)(nei)放置10倍培(pei)養(yang)液，使培(pei)養(yang)液溫(wen)度與室溫(wen)一(yi) 致。1分(fen)鐘內(nei)(nei)融化(hua)細胞(bao)(禁止搖晃凍存管)，移取至試管內(nei)(nei)，動(dong)作要輕，從試管管底開始慢慢上(shang)提(ti) 移液管，使細胞(bao)在培(pei)養(yang)液內(nei)(nei)分(fen)布均勻，離心，洗2次。

相(xiang)關熱賣產品(pin)：

其(qi)它經(jing)驗概括(kuo)：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)根據是(shi)否附(fu)(fu)(fu)于(yu)支持物(wu)上(shang)生(sheng)(sheng)長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)特性，分為(wei) 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)。 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)(fu)(fu)在(zai)支持物(wu)表面(mian)生(sheng)(sheng)長(chang)，只依賴(lai)貼(tie)(tie)附(fu)(fu)(fu)才能生(sheng)(sheng)長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做(zuo)貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象(xiang)與細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分化有關。 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)分型(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自中胚(pei)層(ceng) 特點(dian)：與體內成(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)態(tai)相(xiang)似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體梭型(xing)(xing)(xing)或不(bu)(bu)規(gui)(gui)則(ze)三角形(xing)(xing)(xing),中央有圓形(xing)(xing)(xing)核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質向外伸(shen)出2～3個長(chang)短不(bu)(bu)同(tong)的(de)(de)(de)(de)(de)(de)(de)(de)突起(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)生(sheng)(sheng)長(chang)時(shi)呈(cheng)放射狀(zhuang),漩渦或火(huo)焰狀(zhuang)走(zou)行。 起(qi)源：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自中胚(pei)層(ceng)間(jian)充質組織。 除真正(zheng)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平(ping)滑肌(ji)、成(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)管內皮(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培養(yang)(yang)時(shi)成(cheng)(cheng)(cheng)為(wei)成(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是(shi)一(yi)種慣稱,與體內細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)同(tong)。 2.上(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自外胚(pei)層(ceng)。 特點(dian)：扁平(ping)不(bu)(bu)規(gui)(gui)則(ze)多角型(xing)(xing)(xing)、圓形(xing)(xing)(xing)核(he)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密相(xiang)連(lian)(lian)成(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖數目增多時(shi),整個上(shang)皮(pi)膜隨之(zhi)移動(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而(er)單獨活動(dong)“ 拉網”現象(xiang)與起(qi)源內外胚(pei)層(ceng)組織有關。 組織: 皮(pi)膚表皮(pi)及其衍生(sheng)(sheng)物(wu)(汗腺、皮(pi)脂腺)消化道分上(shang)皮(pi)、肝、胰、肺泡上(shang)皮(pi)。 3. 游走(zou)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點(dian)：在(zai)支持物(wu)上(shang)散在(zai)生(sheng)(sheng)長(chang),不(bu)(bu)連(lian)(lian)接成(cheng)(cheng)(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質伸(shen)出偽足或突起(qi)呈(cheng)活躍的(de)(de)(de)(de)(de)(de)(de)(de)游走(zou)或變形(xing)(xing)(xing)運動(dong),速度快不(bu)(bu)規(gui)(gui)則(ze), 密度大連(lian)(lian)接成(cheng)(cheng)(cheng)片呈(cheng)多角形(xing)(xing)(xing)不(bu)(bu)易與其他類(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別(bie)。 4. 多形(xing)(xing)(xing)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點(dian)： 無(wu)規(gui)(gui)律的(de)(de)(de)(de)(de)(de)(de)(de)形(xing)(xing)(xing)態(tai)，如神(shen)經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing) 特點(dian)： 不(bu)(bu)貼(tie)(tie)附(fu)(fu)(fu)在(zai)支持物(wu)生(sheng)(sheng)長(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體圓形(xing)(xing)(xing)，在(zai)培養(yang)(yang)液中生(sheng)(sheng)長(chang)空間(jian)大，可(ke)(ke)長(chang)時(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)長(chang)，繁殖旺盛便于(yu)做(zuo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研(yan)究。S180肉瘤(liu)、血(xue)液里(li)的(de)(de)(de)(de)(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)： 方(fang)便描述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)培養(yang)(yang)過程中的(de)(de)(de)(de)(de)(de)(de)(de)形(xing)(xing)(xing)態(tai)變化。培養(yang)(yang)條件好時(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相(xiang)對穩定(ding)(ding)，可(ke)(ke)反映出起(qi)源、正(zheng)常異常的(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)，作為(wei)判定(ding)(ding)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)物(wu)學性狀(zhuang)指標。 強調：一(yi)般形(xing)(xing)(xing)態(tai)并不(bu)(bu)是(shi)一(yi)項可(ke)(ke)靠(kao)指標要受各方(fang)面(mian)影響。 如：反復開關溫(wen)箱、溫(wen)度、C02的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度、培養(yang)(yang)基變堿(jian)、支原體、pH值(zhi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)接種時(shi)呈(cheng)三角型(xing)(xing)(xing)狀(zhuang)態(tai)，經培養(yang)(yang)一(yi)定(ding)(ding)時(shi)間(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)傳代前已形(xing)(xing)(xing)成(cheng)(cheng)(cheng)上(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細胞(bao)(bao)，人急性骨(gu)髓白血病細胞(bao)(bao)      25T

MV4-11細(xi)胞(bao)，人急性白血病細(xi)胞(bao)    25T

HL-60細(xi)胞，人急性早(zao)幼粒白血病細(xi)胞    25T

U937細胞，人急性早幼粒白血病細胞     25T

MOLM-13細胞，人(ren)急性(xing)髓(sui)系白血(xue)病(bing)細胞    25T

MOLM-14細(xi)胞，人(ren)急性髓系白血病細(xi)胞    25T

K562細胞(bao)，人慢性髓(sui)系白血病(bing)細胞(bao)  25T

HL-60細胞，人急性(xing)早幼粒白血病細胞    25T

CCRF-CEM細胞(bao)，人急性淋巴細胞(bao)白血病T淋巴細胞(bao)  25T

HS 505.T細胞，慢性淋巴細胞白血病（CLL）細胞   25T