HK-2C人胚腎上皮細胞(bao)，進口胎牛血清慧穎生(sheng)物一站式采購商城(cheng)，細胞株細胞系，耐藥株，STR鑒定，各品(pin)牌進口胎牛血清，培養基(ji)(ji)，胰酶(mei)，雙抗，*培養基(ji)(ji)，PBS緩沖液，DMSO，細胞生(sheng)長(chang)因子(zi)等(deng)，種類(lei)齊(qi)全，*！

細胞(bao)培(pei)養(yang)技(ji)(ji)術屬(shu)于(yu)細胞(bao)工程范疇，細胞(bao)工程包括細胞(bao)融(rong)合技(ji)(ji)術、單克隆(long)(long)抗體的制備、核移植技(ji)(ji)術（克隆(long)(long)）等,而(er)這(zhe)些(xie)技(ji)(ji)術中都(dou)有細胞(bao)培(pei)養(yang)過程,所以說細胞(bao)培(pei)養(yang)是生物學(xue)研(yan)究的zui基本技(ji)(ji)術之一。

上海(hai)慧穎(ying)生(sheng)物供應HK-2C人胚腎(shen)上皮細胞，進口胎牛血清，活性好、存活率(lv)高。隨著細(xi)胞生物學尤其是干細(xi)胞研究的(de)快速發展，在(zai)細(xi)胞治(zhi)療領域已經(jing)進行了(le)(le)大量的(de)探索(suo)，取得了(le)(le)一系列(lie)的(de)令(ling)人(ren)振奮的(de)成果(guo)，且部(bu)分基礎研究成果(guo)已經(jing)在(zai)心血管系統疾(ji)(ji)病(bing) 、神經(jing)系統疾(ji)(ji)病(bing)、肌肉骨骼相關疾(ji)(ji)病(bing)、糖尿病(bing)等。

養(yang)細胞經(jing)驗(yan)分享(xiang)：

1.細胞形態(tai)觀察。細胞膜是每天必(bi)須(xu)觀察的(de)(de)，看是否(fou)清晰，細胞的(de)(de)死亡往(wang)往(wang)是從細胞膜的(de)(de)破(po)裂開始的(de)(de)，早(zao)期的(de)(de)表現(xian)就是細胞膜某(mou)一(yi)點(dian)出現(xian)欠(qian)完整或者毛發影(ying)。

2.細胞(bao)往往會有“抱團”現象，抱團生長(chang)或(huo)死亡，個人認為應該勤換液或(huo)傳代，盡量避 免(mian)細胞(bao)抱團。

3.細(xi)胞(bao)生長(chang)迅速，強烈建議(yi)用較大的(de)培養瓶(ping)養，用小瓶(ping)養的(de)話(hua)，換液不及時，很容易(yi)死 亡。細(xi)胞(bao)的(de)生長(chang)速度一般(ban)為2天(tian)左右就需要傳代，并且一般(ban)為一傳三(san)，或一傳四。

4.培(pei)養(yang)液：1640|DMEM|MEM|IMDM|a-MEM培(pei)養(yang)液，1L一般(ban)加(jia)碳酸氫鈉2g，并加(jia)雙(shuang)抗，胎(tai)牛血清(qing)濃度*為(wei)12%，zui低不低于(yu)10%，有(you)些細(xi)胞(bao)對(dui)胎(tai)牛血清(qing)高度依賴(lai)，比如WRO細(xi)胞(bao)，HT-22細(xi)胞(bao)，HA-VSMC細(xi)胞，NK-92細(xi)胞等。培養液PH應調(diao)為7.20-7.40之間(jian)。

5.防污染。L-02細胞生(sheng)長迅速，較(jiao)少發生(sheng)污染，但常(chang)規措(cuo)施應(ying)該(gai)做(zuo)好。比如無菌操作，酒精(jing) 消毒等，培養(yang)瓶口建議過火，包括瓶蓋，應(ying)過火4秒左右。

6.凍存。-20一小時(shi)，-80次夜，液氮長(chang)期保存。強烈建(jian)議液氮長(chang)期保存，盡量不要在-80長(chang)期 保存，死亡率很(hen)高。

7.復蘇。調節水浴(yu)箱溫(wen)度(du)為42℃，并提前在試管(guan)內放置10倍培養(yang)液，使培養(yang)液溫(wen)度(du)與室溫(wen)一 致。1分(fen)鐘內融化細胞(禁止搖(yao)晃凍存管(guan))，移取至試管(guan)內，動作要輕(qing)，從試管(guan)管(guan)底(di)開始慢(man)慢(man)上提 移液管(guan)，使細胞在培養(yang)液內分(fen)布(bu)均勻，離心，洗2次。

相(xiang)關熱賣產品：

其(qi)它經驗概括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing)根據是否附(fu)于支持(chi)物上(shang)(shang)(shang)生(sheng)長的(de)(de)(de)(de)特(te)性(xing)，分為(wei) 貼(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)(xing)(xing)。 貼(tie)附(fu)型(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)附(fu)在支持(chi)物表(biao)(biao)面生(sheng)長，只依賴貼(tie)附(fu)才能生(sheng)長的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做(zuo)貼(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現(xian)象(xiang)與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分化(hua)有關(guan)。 貼(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)分型(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自中(zhong)胚層(ceng) 特(te)點(dian)：與(yu)體(ti)內(nei)成(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)態相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)(xing)(xing)或(huo)不(bu)規則三角(jiao)形(xing)(xing)(xing)(xing),中(zhong)央有圓形(xing)(xing)(xing)(xing)核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向外伸出(chu)2～3個(ge)(ge)長短不(bu)同(tong)的(de)(de)(de)(de)突(tu)起(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在生(sheng)長時(shi)(shi)(shi)(shi)(shi)呈放射狀(zhuang),漩渦或(huo)火焰狀(zhuang)走行(xing)。 起(qi)源(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自中(zhong)胚層(ceng)間(jian)充質(zhi)組(zu)織(zhi)。 除真正的(de)(de)(de)(de)成(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平(ping)滑肌(ji)、成(cheng)(cheng)骨(gu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)管(guan)內(nei)皮細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注(zhu)意(yi)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在培養(yang)時(shi)(shi)(shi)(shi)(shi)成(cheng)(cheng)為(wei)成(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一種慣稱,與(yu)體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)同(tong)。 2.上(shang)(shang)(shang)皮型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自外胚層(ceng)。 特(te)點(dian)：扁平(ping)不(bu)規則多(duo)角(jiao)型(xing)(xing)(xing)(xing)(xing)、圓形(xing)(xing)(xing)(xing)核、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密相連成(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖數目增多(duo)時(shi)(shi)(shi)(shi)(shi),整個(ge)(ge)上(shang)(shang)(shang)皮膜隨之移動。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活動“ 拉網”現(xian)象(xiang)與(yu)起(qi)源(yuan)內(nei)外胚層(ceng)組(zu)織(zhi)有關(guan)。 組(zu)織(zhi): 皮膚表(biao)(biao)皮及其衍生(sheng)物(汗腺、皮脂(zhi)腺)消化(hua)道分上(shang)(shang)(shang)皮、肝、胰、肺泡上(shang)(shang)(shang)皮。 3. 游走型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點(dian)：在支持(chi)物上(shang)(shang)(shang)散在生(sheng)長,不(bu)連接成(cheng)(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸出(chu)偽足或(huo)突(tu)起(qi)呈活躍的(de)(de)(de)(de)游走或(huo)變形(xing)(xing)(xing)(xing)運(yun)動,速(su)度(du)快不(bu)規則, 密度(du)大連接成(cheng)(cheng)片呈多(duo)角(jiao)形(xing)(xing)(xing)(xing)不(bu)易與(yu)其他類型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別。 4. 多(duo)形(xing)(xing)(xing)(xing)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點(dian)： 無規律的(de)(de)(de)(de)形(xing)(xing)(xing)(xing)態，如(ru)神(shen)經(jing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing)(xing)(xing) 特(te)點(dian)： 不(bu)貼(tie)附(fu)在支持(chi)物生(sheng)長，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓形(xing)(xing)(xing)(xing)，在培養(yang)液中(zhong)生(sheng)長空間(jian)大，可長時(shi)(shi)(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)生(sheng)長，繁殖旺盛便(bian)于做(zuo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝研究。S180肉瘤、血(xue)液里的(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)目的(de)(de)(de)(de)： 方(fang)便(bian)描述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在培養(yang)過程中(zhong)的(de)(de)(de)(de)形(xing)(xing)(xing)(xing)態變化(hua)。培養(yang)條件好時(shi)(shi)(shi)(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩定，可反映(ying)出(chu)起(qi)源(yuan)、正常(chang)異常(chang)的(de)(de)(de)(de)區別，作(zuo)為(wei)判定細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)物學性(xing)狀(zhuang)指標(biao)。 強(qiang)調：一般形(xing)(xing)(xing)(xing)態并不(bu)是一項(xiang)可靠指標(biao)要受各方(fang)面影響。 如(ru)：反復開關(guan)溫(wen)箱、溫(wen)度(du)、C02的(de)(de)(de)(de)濃度(du)、培養(yang)基變堿、支原(yuan)體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在接種時(shi)(shi)(shi)(shi)(shi)呈三角(jiao)型(xing)(xing)(xing)(xing)(xing)狀(zhuang)態，經(jing)培養(yang)一定時(shi)(shi)(shi)(shi)(shi)間(jian)后(hou)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在傳代(dai)前已形(xing)(xing)(xing)(xing)成(cheng)(cheng)上(shang)(shang)(shang)皮型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

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