NRK腎小(xiao)管上皮細胞，進(jin)口(kou)胎牛血清培養慧穎(ying)生(sheng)物一站式采購(gou)商城，細胞株細胞系，耐藥株，STR鑒(jian)定，各品牌進(jin)口胎牛血(xue)清(qing)，培養基，胰酶，雙抗(kang)，*培養基，PBS緩(huan)沖液，DMSO，細胞生(sheng)長因子等，種類齊全，*！

細(xi)胞(bao)(bao)培(pei)(pei)養(yang)技(ji)(ji)(ji)術(shu)屬于細(xi)胞(bao)(bao)工程范疇(chou)，細(xi)胞(bao)(bao)工程包(bao)括細(xi)胞(bao)(bao)融合(he)技(ji)(ji)(ji)術(shu)、單克隆抗體的制備、核移植技(ji)(ji)(ji)術(shu)（克隆）等(deng),而這些技(ji)(ji)(ji)術(shu)中都有(you)細(xi)胞(bao)(bao)培(pei)(pei)養(yang)過程,所以說細(xi)胞(bao)(bao)培(pei)(pei)養(yang)是生(sheng)物(wu)學研究的zui基本技(ji)(ji)(ji)術(shu)之一。

上海慧穎生物供應NRK腎小管上(shang)皮細胞，進口胎牛血清培養，活(huo)性好、存活(huo)率(lv)高。隨著細胞生物(wu)學(xue)尤其是干(gan)細胞研究(jiu)的(de)快速發展，在細胞治療領域已(yi)經(jing)進行了大(da)量的(de)探索，取(qu)得了一系(xi)列的(de)令(ling)人振奮的(de)成果，且部(bu)分基礎研究(jiu)成果已(yi)經(jing)在心血管系(xi)統疾病 、神經(jing)系(xi)統疾病、肌(ji)肉骨骼相關疾病、糖尿病等(deng)。

養細胞經驗分享：

1.細胞(bao)形態觀(guan)察。細胞(bao)膜是(shi)每天必須(xu)觀(guan)察的，看是(shi)否清晰，細胞(bao)的死亡往往是(shi)從細胞(bao)膜的破裂(lie)開始(shi)的，早(zao)期(qi)的表現(xian)就(jiu)是(shi)細胞(bao)膜某一(yi)點出現(xian)欠完(wan)整或者毛(mao)發(fa)影(ying)。

2.細(xi)胞往往會有“抱團”現象，抱團生(sheng)長或死(si)亡(wang)，個人認為應該勤(qin)換液或傳代，盡量(liang)避 免細(xi)胞抱團。

3.細(xi)胞生長(chang)迅速(su)，強烈建議(yi)用較(jiao)大的培(pei)養瓶(ping)養，用小(xiao)瓶(ping)養的話，換液不及(ji)時，很(hen)容易(yi)死 亡。細(xi)胞的生長(chang)速(su)度一(yi)般為2天左右(you)就需要傳代，并(bing)且一(yi)般為一(yi)傳三(san)，或一(yi)傳四。

4.培(pei)養(yang)液(ye)(ye)：1640|DMEM|MEM|IMDM|a-MEM培(pei)養(yang)液(ye)(ye)，1L一(yi)般加(jia)碳酸氫(qing)鈉2g，并加(jia)雙抗(kang)，胎牛血(xue)(xue)清濃度(du)*為(wei)12%，zui低不(bu)低于10%，有些細(xi)胞(bao)對胎牛血(xue)(xue)清高(gao)度(du)依(yi)賴，比如WRO細(xi)胞(bao)，HT-22細(xi)胞(bao)，HA-VSMC細胞，NK-92細胞等。培養液PH應調為7.20-7.40之間。

5.防污染。L-02細胞(bao)生長迅速，較少發生污染，但常規措施應(ying)該做好。比(bi)如(ru)無(wu)菌(jun)操作(zuo)，酒精 消毒(du)等，培(pei)養瓶口建議(yi)過火(huo)，包括瓶蓋，應(ying)過火(huo)4秒左右(you)。

6.凍存(cun)。-20一小時，-80次(ci)夜，液氮長期保存(cun)。強烈(lie)建議液氮長期保存(cun)，盡量不要在(zai)-80長期 保存(cun)，死亡率很高。

7.復蘇。調節水浴箱溫度(du)為42℃，并(bing)提前(qian)在試(shi)(shi)管(guan)(guan)內(nei)放置10倍培(pei)養(yang)液(ye)，使(shi)培(pei)養(yang)液(ye)溫度(du)與室溫一 致。1分(fen)鐘(zhong)內(nei)融(rong)化細胞(禁(jin)止搖晃凍(dong)存管(guan)(guan))，移(yi)取至(zhi)試(shi)(shi)管(guan)(guan)內(nei)，動作要輕，從試(shi)(shi)管(guan)(guan)管(guan)(guan)底開始慢慢上提 移(yi)液(ye)管(guan)(guan)，使(shi)細胞在培(pei)養(yang)液(ye)內(nei)分(fen)布均勻(yun)，離心，洗(xi)2次(ci)。

相關(guan)熱賣產(chan)品：

其(qi)它經驗概(gai)括：

細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)(xing)根據(ju)是(shi)否附(fu)于(yu)支持(chi)物上(shang)(shang)生(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)特(te)性，分(fen)為 貼(tie)(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)、懸浮(fu)型(xing)(xing)(xing)(xing)(xing)。 貼(tie)(tie)附(fu)型(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)在(zai)(zai)支持(chi)物表(biao)面生(sheng)(sheng)長(chang)(chang)(chang)(chang)，只依賴貼(tie)(tie)附(fu)才能(neng)生(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫(jiao)做貼(tie)(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現(xian)象與細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化有(you)(you)關。 貼(tie)(tie)附(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)分(fen)型(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)纖(xian)(xian)(xian)維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自(zi)中胚(pei)層 特(te)點：與體(ti)內(nei)成(cheng)(cheng)纖(xian)(xian)(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形態相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)(xing)(xing)或(huo)(huo)不(bu)(bu)規則三角形,中央有(you)(you)圓形核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向外(wai)伸(shen)出2～3個長(chang)(chang)(chang)(chang)短不(bu)(bu)同的(de)(de)(de)(de)突起。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)生(sheng)(sheng)長(chang)(chang)(chang)(chang)時(shi)呈放射狀(zhuang)(zhuang),漩渦或(huo)(huo)火焰狀(zhuang)(zhuang)走行。 起源：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自(zi)中胚(pei)層間(jian)充質(zhi)組(zu)織。 除真(zhen)正的(de)(de)(de)(de)成(cheng)(cheng)纖(xian)(xian)(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌、平滑肌、成(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)管內(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培(pei)(pei)(pei)養時(shi)成(cheng)(cheng)為成(cheng)(cheng)纖(xian)(xian)(xian)維(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是(shi)一種慣(guan)稱,與體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)同。 2.上(shang)(shang)皮(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自(zi)外(wai)胚(pei)層。 特(te)點：扁平不(bu)(bu)規則多(duo)角型(xing)(xing)(xing)(xing)(xing)、圓形核、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相連(lian)成(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖數目增多(duo)時(shi),整個上(shang)(shang)皮(pi)(pi)膜隨之移動。邊緣細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活動“ 拉(la)網”現(xian)象與起源內(nei)外(wai)胚(pei)層組(zu)織有(you)(you)關。 組(zu)織: 皮(pi)(pi)膚表(biao)皮(pi)(pi)及其衍(yan)生(sheng)(sheng)物(汗腺、皮(pi)(pi)脂腺)消(xiao)化道分(fen)上(shang)(shang)皮(pi)(pi)、肝、胰(yi)、肺泡(pao)上(shang)(shang)皮(pi)(pi)。 3. 游走型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點：在(zai)(zai)支持(chi)物上(shang)(shang)散在(zai)(zai)生(sheng)(sheng)長(chang)(chang)(chang)(chang),不(bu)(bu)連(lian)接成(cheng)(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸(shen)出偽足或(huo)(huo)突起呈活躍的(de)(de)(de)(de)游走或(huo)(huo)變形運(yun)動,速度(du)快(kuai)不(bu)(bu)規則, 密(mi)度(du)大連(lian)接成(cheng)(cheng)片呈多(duo)角形不(bu)(bu)易與其他(ta)類(lei)型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別。 4. 多(duo)形型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點： 無(wu)規律的(de)(de)(de)(de)形態，如神(shen)經(jing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮(fu)型(xing)(xing)(xing)(xing)(xing) 特(te)點： 不(bu)(bu)貼(tie)(tie)附(fu)在(zai)(zai)支持(chi)物生(sheng)(sheng)長(chang)(chang)(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓形，在(zai)(zai)培(pei)(pei)(pei)養液中生(sheng)(sheng)長(chang)(chang)(chang)(chang)空間(jian)大，可(ke)長(chang)(chang)(chang)(chang)時(shi)間(jian)的(de)(de)(de)(de)生(sheng)(sheng)長(chang)(chang)(chang)(chang)，繁殖旺盛(sheng)便于(yu)做細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝研究(jiu)。S180肉瘤、血(xue)液里的(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)目的(de)(de)(de)(de)： 方便描述細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培(pei)(pei)(pei)養過程中的(de)(de)(de)(de)形態變化。培(pei)(pei)(pei)養條件好(hao)時(shi)，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對(dui)穩定(ding)，可(ke)反(fan)映出起源、正常異常的(de)(de)(de)(de)區(qu)別，作(zuo)為判定(ding)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)物學性狀(zhuang)(zhuang)指(zhi)標。 強(qiang)調：一般(ban)形態并不(bu)(bu)是(shi)一項可(ke)靠指(zhi)標要(yao)受各方面影響(xiang)。 如：反(fan)復(fu)開關溫箱、溫度(du)、C02的(de)(de)(de)(de)濃度(du)、培(pei)(pei)(pei)養基變堿、支原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)接種時(shi)呈三角型(xing)(xing)(xing)(xing)(xing)狀(zhuang)(zhuang)態，經(jing)培(pei)(pei)(pei)養一定(ding)時(shi)間(jian)后，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)傳代(dai)前已形成(cheng)(cheng)上(shang)(shang)皮(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細(xi)胞，人急性骨(gu)髓白血病細(xi)胞      25T

MV4-11細(xi)胞，人急性(xing)白血病細(xi)胞    25T

HL-60細(xi)胞，人(ren)急性早(zao)幼(you)粒(li)白(bai)血病細(xi)胞    25T

U937細(xi)胞，人(ren)急性(xing)早幼粒白血病細(xi)胞     25T

MOLM-13細胞，人急性髓系白血病細胞    25T

MOLM-14細胞，人急性髓(sui)系白血病細胞    25T

K562細胞，人慢性(xing)髓系白血病細胞  25T

HL-60細胞，人急性早幼粒(li)白血病細胞    25T

CCRF-CEM細胞(bao)，人急性淋(lin)巴(ba)細胞(bao)白血(xue)病T淋(lin)巴(ba)細胞(bao)  25T

HS 505.T細(xi)(xi)胞(bao)，慢性(xing)淋巴細(xi)(xi)胞(bao)白血病（CLL）細(xi)(xi)胞(bao)   25T