PANC-1胰腺導管上皮癌細胞，進口胎牛血清培(pei)養慧穎生(sheng)物一(yi)站(zhan)式(shi)采購(gou)商城，細(xi)胞株細(xi)胞系，耐(nai)藥株，STR鑒(jian)定，各品(pin)牌進口胎牛血清，培養基(ji)，胰(yi)酶，雙抗，*培養基(ji)，PBS緩沖液，DMSO，細(xi)胞生(sheng)長因子(zi)等，種類齊全，*！

細(xi)胞(bao)培養(yang)技(ji)(ji)術(shu)屬于細(xi)胞(bao)工(gong)程(cheng)范疇，細(xi)胞(bao)工(gong)程(cheng)包括(kuo)細(xi)胞(bao)融合技(ji)(ji)術(shu)、單克隆(long)抗體的制備、核移植技(ji)(ji)術(shu)（克隆(long)）等,而這(zhe)些技(ji)(ji)術(shu)中(zhong)都有(you)細(xi)胞(bao)培養(yang)過程(cheng),所(suo)以說(shuo)細(xi)胞(bao)培養(yang)是生物學研究(jiu)的zui基本(ben)技(ji)(ji)術(shu)之一。

上海慧穎(ying)生物(wu)供應PANC-1胰(yi)腺(xian)導管上皮癌細胞，進口(kou)胎牛血清培(pei)養，活性好(hao)、存活率高。隨著細(xi)胞生物學(xue)尤其是干細(xi)胞研究(jiu)(jiu)的(de)快(kuai)速發(fa)展，在(zai)細(xi)胞治療領域已經(jing)(jing)進行(xing)了大量的(de)探索(suo)，取得了一系列(lie)的(de)令人振奮(fen)的(de)成果(guo)(guo)，且部分基(ji)礎(chu)研究(jiu)(jiu)成果(guo)(guo)已經(jing)(jing)在(zai)心血管系統疾(ji)病(bing) 、神經(jing)(jing)系統疾(ji)病(bing)、肌肉骨骼相關疾(ji)病(bing)、糖尿(niao)病(bing)等。

養(yang)細(xi)胞經驗分享：

1.細(xi)胞(bao)形態觀察(cha)。細(xi)胞(bao)膜(mo)是每天必須觀察(cha)的(de)，看是否清晰(xi)，細(xi)胞(bao)的(de)死亡往往是從細(xi)胞(bao)膜(mo)的(de)破(po)裂開始的(de)，早期的(de)表(biao)現就是細(xi)胞(bao)膜(mo)某一點出現欠(qian)完整或(huo)者(zhe)毛發影。

2.細胞往(wang)往(wang)會有“抱(bao)團(tuan)”現(xian)象(xiang)，抱(bao)團(tuan)生長(chang)或(huo)死亡，個人認(ren)為(wei)應(ying)該(gai)勤換(huan)液(ye)或(huo)傳代，盡量避 免細胞抱(bao)團(tuan)。

3.細(xi)胞(bao)生(sheng)長(chang)迅(xun)速(su)，強(qiang)烈建議用(yong)較大的培養(yang)(yang)瓶(ping)(ping)養(yang)(yang)，用(yong)小瓶(ping)(ping)養(yang)(yang)的話，換液不及時(shi)，很容易死 亡。細(xi)胞(bao)的生(sheng)長(chang)速(su)度一(yi)(yi)般為(wei)2天(tian)左右(you)就需要傳代，并且一(yi)(yi)般為(wei)一(yi)(yi)傳三，或一(yi)(yi)傳四(si)。

4.培(pei)養液：1640|DMEM|MEM|IMDM|a-MEM培(pei)養液，1L一般加碳酸氫鈉2g，并加雙抗，胎牛血清(qing)濃度*為(wei)12%，zui低不低于10%，有些(xie)細(xi)胞(bao)對胎牛血清(qing)高度依賴，比如WRO細(xi)胞(bao)，HT-22細(xi)胞(bao)，HA-VSMC細(xi)胞，NK-92細(xi)胞等(deng)。培養液PH應調為7.20-7.40之間。

5.防(fang)污染(ran)。L-02細胞生長迅速，較少發生污染(ran)，但常規措(cuo)施(shi)應該做(zuo)好。比(bi)如無菌操作，酒精 消毒等，培養瓶口建議(yi)過(guo)(guo)火，包括瓶蓋，應過(guo)(guo)火4秒(miao)左(zuo)右。

6.凍存(cun)。-20一(yi)小(xiao)時(shi)，-80次夜，液氮(dan)長期(qi)保(bao)存(cun)。強烈建議(yi)液氮(dan)長期(qi)保(bao)存(cun)，盡量(liang)不(bu)要在-80長期(qi) 保(bao)存(cun)，死亡率很高。

7.復蘇(su)。調節水浴箱溫(wen)度為42℃，并提前在(zai)試(shi)管內(nei)(nei)放置(zhi)10倍培養(yang)液(ye)，使(shi)培養(yang)液(ye)溫(wen)度與室溫(wen)一 致(zhi)。1分(fen)(fen)鐘內(nei)(nei)融化細胞(bao)(bao)(禁止搖晃(huang)凍存管)，移取至試(shi)管內(nei)(nei)，動(dong)作要輕，從試(shi)管管底開始慢慢上提 移液(ye)管，使(shi)細胞(bao)(bao)在(zai)培養(yang)液(ye)內(nei)(nei)分(fen)(fen)布(bu)均勻，離心，洗(xi)2次。

相關熱(re)賣產品：

其它經驗概括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)根據(ju)是否附于支持物上(shang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)特(te)性，分(fen)為(wei) 貼(tie)(tie)附型(xing)(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)(xing)。 貼(tie)(tie)附型(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附在支持物表(biao)面生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，只依(yi)賴(lai)貼(tie)(tie)附才能生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)(tie)附型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現(xian)象與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化(hua)有關。 貼(tie)(tie)附型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)分(fen)型(xing)(xing)(xing)(xing) 1.成(cheng)纖維型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自中(zhong)胚層 特(te)點(dian)(dian)：與(yu)體(ti)(ti)內(nei)成(cheng)纖維細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)(xing)態(tai)相似,胞(bao)(bao)(bao)(bao)(bao)(bao)體(ti)(ti)梭型(xing)(xing)(xing)(xing)或(huo)不(bu)(bu)(bu)規(gui)則三角(jiao)形(xing)(xing)(xing)(xing)(xing),中(zhong)央有圓形(xing)(xing)(xing)(xing)(xing)核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向外伸(shen)出2～3個(ge)長(chang)(chang)短不(bu)(bu)(bu)同的(de)(de)(de)突起(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)時(shi)(shi)呈(cheng)(cheng)放射狀(zhuang),漩渦或(huo)火(huo)焰狀(zhuang)走行。 起(qi)源(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自中(zhong)胚層間(jian)充質(zhi)組織。 除(chu)真正的(de)(de)(de)成(cheng)纖維細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平(ping)滑肌(ji)、成(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、血管內(nei)皮(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在培(pei)養(yang)時(shi)(shi)成(cheng)為(wei)成(cheng)纖維型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)(yi)種慣稱,與(yu)體(ti)(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)同。 2.上(shang)皮(pi)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自外胚層。 特(te)點(dian)(dian)：扁平(ping)不(bu)(bu)(bu)規(gui)則多角(jiao)型(xing)(xing)(xing)(xing)、圓形(xing)(xing)(xing)(xing)(xing)核(he)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)緊(jin)密相連成(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)增殖數目(mu)增多時(shi)(shi),整個(ge)上(shang)皮(pi)膜隨之移動(dong)。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)很(hen)少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)群(qun)而單獨活動(dong)“ 拉(la)網”現(xian)象與(yu)起(qi)源(yuan)內(nei)外胚層組織有關。 組織: 皮(pi)膚表(biao)皮(pi)及其衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(汗腺、皮(pi)脂腺)消化(hua)道分(fen)上(shang)皮(pi)、肝、胰(yi)、肺泡上(shang)皮(pi)。 3. 游走型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點(dian)(dian)：在支持物上(shang)散在生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang),不(bu)(bu)(bu)連接成(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸(shen)出偽足或(huo)突起(qi)呈(cheng)(cheng)活躍的(de)(de)(de)游走或(huo)變形(xing)(xing)(xing)(xing)(xing)運(yun)動(dong),速度(du)快不(bu)(bu)(bu)規(gui)則, 密度(du)大連接成(cheng)片(pian)呈(cheng)(cheng)多角(jiao)形(xing)(xing)(xing)(xing)(xing)不(bu)(bu)(bu)易與(yu)其他類(lei)型(xing)(xing)(xing)(xing)的(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao) 區別(bie)。 4. 多形(xing)(xing)(xing)(xing)(xing)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點(dian)(dian)： 無(wu)規(gui)律(lv)的(de)(de)(de)形(xing)(xing)(xing)(xing)(xing)態(tai)，如神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing)(xing) 特(te)點(dian)(dian)： 不(bu)(bu)(bu)貼(tie)(tie)附在支持物生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)體(ti)(ti)圓形(xing)(xing)(xing)(xing)(xing)，在培(pei)養(yang)液中(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)空間(jian)大，可(ke)長(chang)(chang)時(shi)(shi)間(jian)的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，繁殖旺盛(sheng)便于做細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝(xie)研究。S180肉瘤、血液里的(de)(de)(de)白(bai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)的(de)(de)(de)目(mu)的(de)(de)(de)： 方便描(miao)述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在培(pei)養(yang)過程中(zhong)的(de)(de)(de)形(xing)(xing)(xing)(xing)(xing)態(tai)變化(hua)。培(pei)養(yang)條件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)相對穩定，可(ke)反(fan)映出起(qi)源(yuan)、正常(chang)(chang)異常(chang)(chang)的(de)(de)(de)區別(bie)，作為(wei)判定細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物學性狀(zhuang)指(zhi)標。 強調：一(yi)(yi)般形(xing)(xing)(xing)(xing)(xing)態(tai)并不(bu)(bu)(bu)是一(yi)(yi)項可(ke)靠指(zhi)標要受(shou)各方面影響(xiang)。 如：反(fan)復(fu)開關溫箱(xiang)、溫度(du)、C02的(de)(de)(de)濃度(du)、培(pei)養(yang)基變堿、支原體(ti)(ti)、pH值(zhi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在接種時(shi)(shi)呈(cheng)(cheng)三角(jiao)型(xing)(xing)(xing)(xing)狀(zhuang)態(tai)，經培(pei)養(yang)一(yi)(yi)定時(shi)(shi)間(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)在傳代(dai)前(qian)已(yi)形(xing)(xing)(xing)(xing)(xing)成(cheng)上(shang)皮(pi)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。

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