BXPC-3原(yuan)位胰腺(xian)癌細胞, 進口胎牛血清慧穎生(sheng)物一站式(shi)采購商(shang)城，細胞(bao)(bao)株細胞(bao)(bao)系，耐藥株，STR鑒定，各品牌進口(kou)胎牛血清(qing)，培養(yang)基，胰酶，雙抗，*培養(yang)基，PBS緩沖(chong)液(ye)，DMSO，細胞(bao)(bao)生(sheng)長(chang)因(yin)子等(deng)，種類齊(qi)全，*！

細(xi)(xi)胞(bao)培(pei)(pei)養技術屬于細(xi)(xi)胞(bao)工程范疇(chou)，細(xi)(xi)胞(bao)工程包(bao)括細(xi)(xi)胞(bao)融(rong)合技術、單(dan)克隆(long)(long)抗(kang)體的制備(bei)、核移(yi)植技術（克隆(long)(long)）等,而這些技術中都(dou)有(you)細(xi)(xi)胞(bao)培(pei)(pei)養過程,所以說細(xi)(xi)胞(bao)培(pei)(pei)養是生物學研究的zui基本(ben)技術之一。

上海慧穎生物供應BXPC-3原位胰(yi)腺(xian)癌細胞(bao), 進(jin)口胎牛血清(qing)，活(huo)性好(hao)、存(cun)活(huo)率(lv)高。隨著細(xi)(xi)胞生物學尤其是干細(xi)(xi)胞研究的(de)(de)快速發展，在(zai)(zai)細(xi)(xi)胞治療領域已(yi)經(jing)(jing)進行(xing)了(le)大(da)量的(de)(de)探索，取得了(le)一(yi)系(xi)列的(de)(de)令人(ren)振(zhen)奮的(de)(de)成(cheng)果，且部分基礎研究成(cheng)果已(yi)經(jing)(jing)在(zai)(zai)心血管(guan)系(xi)統疾病 、神經(jing)(jing)系(xi)統疾病、肌肉骨骼(ge)相(xiang)關疾病、糖(tang)尿病等。

養(yang)細胞經(jing)驗分享：

1.細(xi)胞(bao)(bao)形態(tai)觀察(cha)。細(xi)胞(bao)(bao)膜是每天必(bi)須(xu)觀察(cha)的(de)(de)，看是否清晰，細(xi)胞(bao)(bao)的(de)(de)死亡往(wang)往(wang)是從細(xi)胞(bao)(bao)膜的(de)(de)破(po)裂開始(shi)的(de)(de)，早期的(de)(de)表現(xian)就是細(xi)胞(bao)(bao)膜某(mou)一點出現(xian)欠(qian)完(wan)整或者(zhe)毛發影。

2.細胞往往會有(you)“抱團(tuan)”現象，抱團(tuan)生長(chang)或死(si)亡，個人(ren)認為應(ying)該勤(qin)換液(ye)或傳代，盡量避 免細胞抱團(tuan)。

3.細(xi)(xi)胞(bao)生(sheng)長迅速，強烈建議(yi)用較大的培養瓶養，用小瓶養的話，換(huan)液不(bu)及時，很容易死 亡(wang)。細(xi)(xi)胞(bao)的生(sheng)長速度一(yi)(yi)般為(wei)2天左右(you)就需要傳(chuan)(chuan)代，并且一(yi)(yi)般為(wei)一(yi)(yi)傳(chuan)(chuan)三，或一(yi)(yi)傳(chuan)(chuan)四。

4.培養(yang)液：1640|DMEM|MEM|IMDM|a-MEM培養(yang)液，1L一般加碳(tan)酸氫鈉2g，并加雙(shuang)抗，胎牛血清濃度(du)(du)*為12%，zui低(di)不(bu)低(di)于10%，有(you)些細胞(bao)對(dui)胎牛血清高度(du)(du)依賴(lai)，比如WRO細胞(bao)，HT-22細胞(bao)，HA-VSMC細(xi)胞，NK-92細(xi)胞等(deng)。培養液PH應(ying)調為7.20-7.40之間。

5.防污(wu)染。L-02細胞生長迅速，較(jiao)少(shao)發生污(wu)染，但常規措施應該做好。比如無菌(jun)操作，酒精 消(xiao)毒(du)等，培(pei)養(yang)瓶口建議過(guo)火，包括(kuo)瓶蓋，應過(guo)火4秒左右(you)。

6.凍(dong)存(cun)(cun)。-20一小時(shi)，-80次夜(ye)，液氮長(chang)(chang)期(qi)保存(cun)(cun)。強烈(lie)建議液氮長(chang)(chang)期(qi)保存(cun)(cun)，盡量(liang)不要在-80長(chang)(chang)期(qi) 保存(cun)(cun)，死亡率很高。

7.復蘇。調節水浴箱溫(wen)度(du)為42℃，并提前(qian)在試(shi)管(guan)內(nei)放置10倍(bei)培養(yang)液(ye)，使培養(yang)液(ye)溫(wen)度(du)與室溫(wen)一 致。1分鐘(zhong)內(nei)融化細胞(禁止搖晃凍存管(guan))，移(yi)取至試(shi)管(guan)內(nei)，動(dong)作要輕，從試(shi)管(guan)管(guan)底開(kai)始(shi)慢慢上提 移(yi)液(ye)管(guan)，使細胞在培養(yang)液(ye)內(nei)分布均勻，離(li)心，洗(xi)2次。

相關(guan)熱賣產品：

其它(ta)經驗概(gai)括(kuo)：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)(xing)(xing) 編(bian)輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)(xing)(xing)根據是(shi)(shi)(shi)否附(fu)(fu)(fu)(fu)于支(zhi)持物(wu)(wu)上(shang)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)特(te)性，分為 貼(tie)(tie)附(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)(xing)(xing)(xing)。 貼(tie)(tie)附(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)(fu)(fu)(fu)在(zai)(zai)(zai)(zai)支(zhi)持物(wu)(wu)表面生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，只(zhi)依賴貼(tie)(tie)附(fu)(fu)(fu)(fu)才(cai)能生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫(jiao)做(zuo)貼(tie)(tie)附(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種(zhong)(zhong)現象與細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)分化(hua)(hua)有(you)關。 貼(tie)(tie)附(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)分型(xing)(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自中胚層(ceng)(ceng) 特(te)點：與體(ti)內(nei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)形態(tai)相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)(xing)(xing)(xing)或(huo)(huo)不(bu)(bu)(bu)(bu)規(gui)則三角(jiao)形,中央有(you)圓形核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)向(xiang)外伸出(chu)(chu)2～3個長(chang)(chang)短不(bu)(bu)(bu)(bu)同(tong)的(de)(de)(de)(de)(de)突起。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)時(shi)(shi)呈放射狀,漩(xuan)渦或(huo)(huo)火焰(yan)狀走(zou)行。 起源：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自中胚層(ceng)(ceng)間(jian)充質(zhi)(zhi)組(zu)織(zhi)。 除真正的(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平滑肌(ji)、成(cheng)(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管(guan)內(nei)皮細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)培養(yang)(yang)時(shi)(shi)成(cheng)(cheng)(cheng)(cheng)為成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)是(shi)(shi)(shi)一(yi)種(zhong)(zhong)慣稱,與體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)同(tong)。 2.上(shang)皮型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自外胚層(ceng)(ceng)。 特(te)點：扁平不(bu)(bu)(bu)(bu)規(gui)則多角(jiao)型(xing)(xing)(xing)(xing)(xing)(xing)、圓形核(he)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密相連(lian)成(cheng)(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖(zhi)數(shu)目(mu)增多時(shi)(shi),整個上(shang)皮膜(mo)隨(sui)之移(yi)動(dong)。邊緣(yuan)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離(li)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨(du)活(huo)動(dong)“ 拉網”現象與起源內(nei)外胚層(ceng)(ceng)組(zu)織(zhi)有(you)關。 組(zu)織(zhi): 皮膚表皮及其衍生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(汗(han)腺、皮脂腺)消化(hua)(hua)道(dao)分上(shang)皮、肝、胰、肺泡上(shang)皮。 3. 游走(zou)型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點：在(zai)(zai)(zai)(zai)支(zhi)持物(wu)(wu)上(shang)散在(zai)(zai)(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang),不(bu)(bu)(bu)(bu)連(lian)接成(cheng)(cheng)(cheng)(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)伸出(chu)(chu)偽(wei)足或(huo)(huo)突起呈活(huo)躍的(de)(de)(de)(de)(de)游走(zou)或(huo)(huo)變形運(yun)動(dong),速(su)度(du)快不(bu)(bu)(bu)(bu)規(gui)則, 密度(du)大連(lian)接成(cheng)(cheng)(cheng)(cheng)片呈多角(jiao)形不(bu)(bu)(bu)(bu)易與其他類(lei)型(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別。 4. 多形型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點： 無規(gui)律的(de)(de)(de)(de)(de)形態(tai)，如(ru)神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing)(xing)(xing)(xing) 特(te)點： 不(bu)(bu)(bu)(bu)貼(tie)(tie)附(fu)(fu)(fu)(fu)在(zai)(zai)(zai)(zai)支(zhi)持物(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓形，在(zai)(zai)(zai)(zai)培養(yang)(yang)液(ye)中生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)空間(jian)大，可長(chang)(chang)時(shi)(shi)間(jian)的(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)，繁殖(zhi)旺盛便(bian)于做(zuo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉瘤(liu)、血液(ye)里的(de)(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分型(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)目(mu)的(de)(de)(de)(de)(de)： 方(fang)便(bian)描述(shu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)培養(yang)(yang)過程中的(de)(de)(de)(de)(de)形態(tai)變化(hua)(hua)。培養(yang)(yang)條件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對(dui)穩定，可反映(ying)出(chu)(chu)起源、正常異(yi)常的(de)(de)(de)(de)(de)區別，作為判定細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)學性狀指標(biao)。 強調(diao)：一(yi)般形態(tai)并不(bu)(bu)(bu)(bu)是(shi)(shi)(shi)一(yi)項可靠指標(biao)要受各方(fang)面影響。 如(ru)：反復開關溫箱(xiang)、溫度(du)、C02的(de)(de)(de)(de)(de)濃度(du)、培養(yang)(yang)基變堿、支(zhi)原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)接種(zhong)(zhong)時(shi)(shi)呈三角(jiao)型(xing)(xing)(xing)(xing)(xing)(xing)狀態(tai)，經培養(yang)(yang)一(yi)定時(shi)(shi)間(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)傳代前已形成(cheng)(cheng)(cheng)(cheng)上(shang)皮型(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細胞，人急性(xing)骨髓(sui)白(bai)血病細胞      25T

MV4-11細胞(bao)(bao)，人急(ji)性(xing)白血(xue)病(bing)細胞(bao)(bao)    25T

HL-60細(xi)胞(bao)，人急性早幼粒白血病細(xi)胞(bao)    25T

U937細胞(bao)，人急(ji)性早(zao)幼粒(li)白血病細胞(bao)     25T

MOLM-13細胞(bao)，人(ren)急性(xing)髓系(xi)白血病細胞(bao)    25T

MOLM-14細胞，人急性髓系白血病(bing)細胞    25T

K562細胞(bao)，人慢性髓系白血病細胞(bao)  25T

HL-60細(xi)胞(bao)，人急性早幼(you)粒白血病細(xi)胞(bao)    25T

CCRF-CEM細(xi)胞(bao)，人急性淋巴細(xi)胞(bao)白血病T淋巴細(xi)胞(bao)  25T

HS 505.T細(xi)胞，慢性淋巴細(xi)胞白血病(bing)（CLL）細(xi)胞   25T