H520小細胞肺癌(ai)，進口胎牛(niu)血清培(pei)養(yang)慧穎(ying)生物一站(zhan)式采購商城，細(xi)胞株細(xi)胞系，耐藥株，STR鑒定，各(ge)品牌進口胎牛(niu)血清，培養(yang)基，胰酶，雙抗(kang)，*培養(yang)基，PBS緩沖液，DMSO，細(xi)胞生長因子等，種類(lei)齊全，*！

細(xi)胞(bao)培養(yang)技(ji)術屬于細(xi)胞(bao)工(gong)程(cheng)范(fan)疇，細(xi)胞(bao)工(gong)程(cheng)包(bao)括細(xi)胞(bao)融合技(ji)術、單(dan)克隆抗體的制(zhi)備、核移植技(ji)術（克隆）等,而(er)這些技(ji)術中(zhong)都有細(xi)胞(bao)培養(yang)過程(cheng),所以說細(xi)胞(bao)培養(yang)是生物學研究的zui基本技(ji)術之(zhi)一。

上海慧穎(ying)生物供(gong)應(ying)H520小細胞肺癌，進口胎牛血清培養，活性好、存活率高。隨著細胞生物學尤其是干細胞研(yan)究(jiu)(jiu)的(de)快速發展，在細胞治療領域(yu)已經進行了(le)大量的(de)探索，取(qu)得了(le)一(yi)系(xi)(xi)列的(de)令人振奮的(de)成果，且部(bu)分基礎研(yan)究(jiu)(jiu)成果已經在心血管系(xi)(xi)統疾病(bing) 、神(shen)經系(xi)(xi)統疾病(bing)、肌肉骨(gu)骼相關疾病(bing)、糖尿病(bing)等(deng)。

養(yang)細胞經驗分享：

1.細(xi)(xi)胞(bao)(bao)形態觀察(cha)。細(xi)(xi)胞(bao)(bao)膜是每天(tian)必(bi)須觀察(cha)的，看是否清晰(xi)，細(xi)(xi)胞(bao)(bao)的死亡(wang)往往是從(cong)細(xi)(xi)胞(bao)(bao)膜的破裂開始的，早期的表(biao)現就是細(xi)(xi)胞(bao)(bao)膜某一點出現欠完整(zheng)或者毛(mao)發影。

2.細(xi)胞(bao)往往會有“抱(bao)團”現(xian)象(xiang)，抱(bao)團生(sheng)長或死亡，個人(ren)認為應該勤換(huan)液或傳代，盡量避 免(mian)細(xi)胞(bao)抱(bao)團。

3.細胞(bao)(bao)生(sheng)長(chang)迅速，強烈建議用較大的培養(yang)瓶(ping)養(yang)，用小瓶(ping)養(yang)的話(hua)，換液(ye)不及時(shi)，很容易死 亡。細胞(bao)(bao)的生(sheng)長(chang)速度一(yi)般為(wei)2天左右就需要傳(chuan)(chuan)代(dai)，并(bing)且一(yi)般為(wei)一(yi)傳(chuan)(chuan)三(san)，或一(yi)傳(chuan)(chuan)四。

4.培(pei)養液(ye)(ye)：1640|DMEM|MEM|IMDM|a-MEM培(pei)養液(ye)(ye)，1L一(yi)般加碳酸氫(qing)鈉2g，并加雙抗，胎牛(niu)血(xue)清濃度*為12%，zui低(di)不低(di)于10%，有些(xie)細胞對胎牛(niu)血(xue)清高度依(yi)賴，比如WRO細胞，HT-22細胞，HA-VSMC細胞，NK-92細胞等。培養液PH應調為(wei)7.20-7.40之間(jian)。

5.防污(wu)染。L-02細胞(bao)生長迅(xun)速，較(jiao)少發生污(wu)染，但常規措(cuo)施應(ying)該(gai)做好。比(bi)如(ru)無菌操(cao)作，酒精 消毒(du)等(deng)，培養瓶口建(jian)議(yi)過(guo)火，包括瓶蓋，應(ying)過(guo)火4秒左右。

6.凍(dong)存。-20一(yi)小時，-80次夜，液(ye)氮長期保(bao)存。強烈(lie)建議(yi)液(ye)氮長期保(bao)存，盡量不要在-80長期 保(bao)存，死亡率很高(gao)。

7.復蘇。調節水浴箱溫度為42℃，并提(ti)前在試(shi)管內(nei)放置(zhi)10倍培養(yang)液(ye)，使(shi)培養(yang)液(ye)溫度與室(shi)溫一 致。1分鐘內(nei)融化細胞(bao)(禁止搖晃(huang)凍存管)，移取至試(shi)管內(nei)，動作要輕，從(cong)試(shi)管管底(di)開始(shi)慢慢上提(ti) 移液(ye)管，使(shi)細胞(bao)在培養(yang)液(ye)內(nei)分布均勻，離心，洗2次。

相(xiang)關(guan)熱賣(mai)產品(pin)：

其(qi)它經(jing)驗概(gai)括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing) 編輯(ji) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)(xing)根據是否附(fu)(fu)于(yu)支持物(wu)上(shang)(shang)生(sheng)長(chang)(chang)(chang)的特(te)性(xing)，分(fen)為(wei) 貼(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)、懸(xuan)浮型(xing)(xing)(xing)(xing)。 貼(tie)附(fu)(fu)型(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)附(fu)(fu)在(zai)(zai)支持物(wu)表面(mian)生(sheng)長(chang)(chang)(chang)，只依賴貼(tie)附(fu)(fu)才能生(sheng)長(chang)(chang)(chang)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種(zhong)現(xian)象與(yu)(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化(hua)有關(guan)。 貼(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的分(fen)型(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)(cheng)纖維(wei)(wei)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自中(zhong)胚層(ceng) 特(te)點(dian)：與(yu)(yu)體(ti)內(nei)(nei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形態(tai)相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭(suo)型(xing)(xing)(xing)(xing)或(huo)不(bu)(bu)(bu)規(gui)(gui)則(ze)三(san)角(jiao)(jiao)形,中(zhong)央有圓(yuan)形核(he)(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)向外伸出2～3個長(chang)(chang)(chang)短不(bu)(bu)(bu)同的突起(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)生(sheng)長(chang)(chang)(chang)時(shi)(shi)呈放射狀(zhuang),漩渦或(huo)火焰狀(zhuang)走行(xing)。 起(qi)源(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自中(zhong)胚層(ceng)間(jian)充質(zhi)組織(zhi)。 除真正(zheng)的成(cheng)(cheng)(cheng)(cheng)纖維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平滑(hua)肌(ji)、成(cheng)(cheng)(cheng)(cheng)骨(gu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管內(nei)(nei)皮細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培養時(shi)(shi)成(cheng)(cheng)(cheng)(cheng)為(wei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)(wei)型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)(yi)種(zhong)慣稱,與(yu)(yu)體(ti)內(nei)(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)同。 2.上(shang)(shang)皮型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自外胚層(ceng)。 特(te)點(dian)：扁平不(bu)(bu)(bu)規(gui)(gui)則(ze)多(duo)角(jiao)(jiao)型(xing)(xing)(xing)(xing)、圓(yuan)形核(he)(he)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密相連(lian)成(cheng)(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖(zhi)數目增多(duo)時(shi)(shi),整個上(shang)(shang)皮膜(mo)隨之移動。邊(bian)緣(yuan)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單(dan)獨活(huo)動“ 拉(la)網”現(xian)象與(yu)(yu)起(qi)源(yuan)內(nei)(nei)外胚層(ceng)組織(zhi)有關(guan)。 組織(zhi): 皮膚表皮及其衍生(sheng)物(wu)(汗腺、皮脂腺)消化(hua)道分(fen)上(shang)(shang)皮、肝、胰、肺(fei)泡上(shang)(shang)皮。 3. 游走型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點(dian)：在(zai)(zai)支持物(wu)上(shang)(shang)散在(zai)(zai)生(sheng)長(chang)(chang)(chang),不(bu)(bu)(bu)連(lian)接成(cheng)(cheng)(cheng)(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)伸出偽足(zu)或(huo)突起(qi)呈活(huo)躍(yue)的游走或(huo)變(bian)(bian)形運動,速(su)度快(kuai)不(bu)(bu)(bu)規(gui)(gui)則(ze), 密度大連(lian)接成(cheng)(cheng)(cheng)(cheng)片(pian)呈多(duo)角(jiao)(jiao)形不(bu)(bu)(bu)易與(yu)(yu)其他類(lei)型(xing)(xing)(xing)(xing)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別。 4. 多(duo)形型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點(dian)： 無(wu)規(gui)(gui)律的形態(tai)，如(ru)(ru)神經細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸(xuan)浮型(xing)(xing)(xing)(xing) 特(te)點(dian)： 不(bu)(bu)(bu)貼(tie)附(fu)(fu)在(zai)(zai)支持物(wu)生(sheng)長(chang)(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓(yuan)形，在(zai)(zai)培養液中(zhong)生(sheng)長(chang)(chang)(chang)空間(jian)大，可長(chang)(chang)(chang)時(shi)(shi)間(jian)的生(sheng)長(chang)(chang)(chang)，繁(fan)殖(zhi)旺盛便(bian)于(yu)做細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝研(yan)究。S180肉瘤、血液里的白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)的目的： 方(fang)便(bian)描述(shu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培養過程中(zhong)的形態(tai)變(bian)(bian)化(hua)。培養條(tiao)件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩(wen)定(ding)，可反(fan)映出起(qi)源(yuan)、正(zheng)常異常的區別，作為(wei)判定(ding)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)物(wu)學性(xing)狀(zhuang)指(zhi)標(biao)。 強調：一(yi)(yi)般形態(tai)并不(bu)(bu)(bu)是一(yi)(yi)項可靠指(zhi)標(biao)要受各方(fang)面(mian)影響。 如(ru)(ru)：反(fan)復開關(guan)溫箱、溫度、C02的濃度、培養基變(bian)(bian)堿、支原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)接種(zhong)時(shi)(shi)呈三(san)角(jiao)(jiao)型(xing)(xing)(xing)(xing)狀(zhuang)態(tai)，經培養一(yi)(yi)定(ding)時(shi)(shi)間(jian)后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)傳代(dai)前已形成(cheng)(cheng)(cheng)(cheng)上(shang)(shang)皮型(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細胞，人(ren)急性骨髓白血(xue)病(bing)細胞      25T

MV4-11細胞(bao)，人急性(xing)白血病(bing)細胞(bao)    25T

HL-60細胞(bao)，人急性(xing)早(zao)幼粒白(bai)血病細胞(bao)    25T

U937細胞，人急性早幼粒白血病細胞     25T

MOLM-13細胞(bao)，人急性髓系白血(xue)病細胞(bao)    25T

MOLM-14細(xi)胞，人(ren)急性髓系(xi)白血病細(xi)胞    25T

K562細(xi)(xi)胞(bao)，人慢性髓系白血病細(xi)(xi)胞(bao)  25T

HL-60細胞，人急性早幼粒白血病細胞    25T

CCRF-CEM細胞，人急性淋巴細胞白血病T淋巴細胞  25T

HS 505.T細胞，慢(man)性淋(lin)巴細胞白血病（CLL）細胞   25T

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