A549 肺腺癌細(xi)胞慧(hui)穎生物一(yi)站(zhan)式采購商城，細胞(bao)株(zhu)細胞(bao)系，耐藥株(zhu)，STR鑒定，各品牌進口胎牛血清，培養基，胰(yi)酶，雙抗，*培養基，PBS緩沖液，DMSO，細胞(bao)生長因子等(deng)，種類齊全(quan)，*！

細(xi)(xi)胞(bao)培養(yang)技術(shu)屬(shu)于細(xi)(xi)胞(bao)工(gong)程(cheng)范疇，細(xi)(xi)胞(bao)工(gong)程(cheng)包括細(xi)(xi)胞(bao)融合技術(shu)、單克(ke)隆抗體的制備、核移植技術(shu)（克(ke)隆）等,而(er)這些技術(shu)中(zhong)都有細(xi)(xi)胞(bao)培養(yang)過程(cheng),所以說細(xi)(xi)胞(bao)培養(yang)是生物學研究的zui基本技術(shu)之一。

上海(hai)慧(hui)穎生物供應A549 肺腺癌(ai)細胞，活(huo)性(xing)好、存活(huo)率(lv)高。隨著細(xi)胞(bao)生物(wu)學尤其(qi)是干(gan)細(xi)胞(bao)研(yan)(yan)究的快速發展，在細(xi)胞(bao)治療領域已(yi)經進行了(le)大(da)量的探索，取得了(le)一系(xi)列的令人(ren)振奮的成(cheng)果(guo)，且部分基礎研(yan)(yan)究成(cheng)果(guo)已(yi)經在心血管(guan)系(xi)統疾病(bing) 、神(shen)經系(xi)統疾病(bing)、肌肉骨骼(ge)相關疾病(bing)、糖(tang)尿(niao)病(bing)等。

養細(xi)胞(bao)經驗分享：

1.細(xi)(xi)(xi)胞(bao)形態觀察。細(xi)(xi)(xi)胞(bao)膜(mo)是(shi)(shi)每天必須(xu)觀察的(de)(de)，看是(shi)(shi)否清晰，細(xi)(xi)(xi)胞(bao)的(de)(de)死亡往往是(shi)(shi)從細(xi)(xi)(xi)胞(bao)膜(mo)的(de)(de)破裂開始的(de)(de)，早期的(de)(de)表(biao)現(xian)就是(shi)(shi)細(xi)(xi)(xi)胞(bao)膜(mo)某一(yi)點出現(xian)欠完整或(huo)者毛發影。

2.細胞往往會有“抱團(tuan)”現(xian)象，抱團(tuan)生(sheng)長或(huo)死(si)亡，個人認(ren)為應該勤換液或(huo)傳代，盡量避 免細胞抱團(tuan)。

3.細(xi)胞(bao)生長迅(xun)速(su)，強烈建議用較大(da)的(de)培養(yang)瓶養(yang)，用小瓶養(yang)的(de)話，換液不及時，很容易死 亡。細(xi)胞(bao)的(de)生長速(su)度一般為2天左右就需要傳代，并且(qie)一般為一傳三，或一傳四。

4.培(pei)養液(ye)：1640|DMEM|MEM|IMDM|a-MEM培(pei)養液(ye)，1L一般加碳酸氫鈉2g，并加雙抗，胎牛(niu)血清濃度*為12%，zui低不低于10%，有些細(xi)胞對胎牛(niu)血清高(gao)度依(yi)賴，比如WRO細(xi)胞，HT-22細(xi)胞，HA-VSMC細胞(bao)，NK-92細胞(bao)等。培養液PH應調(diao)為(wei)7.20-7.40之間(jian)。

5.防污染。L-02細胞生長迅速，較少發生污染，但(dan)常規(gui)措施應(ying)該(gai)做(zuo)好。比如無(wu)菌(jun)操作，酒精(jing) 消(xiao)毒(du)等，培養(yang)瓶口(kou)建議過火(huo)，包括瓶蓋，應(ying)過火(huo)4秒左右。

6.凍存(cun)。-20一小時(shi)，-80次夜，液氮長期保存(cun)。強烈(lie)建議液氮長期保存(cun)，盡量不要在-80長期 保存(cun)，死亡率(lv)很高。

7.復蘇。調節(jie)水浴箱溫(wen)度(du)為(wei)42℃，并提(ti)前在試管(guan)內放置(zhi)10倍培(pei)養(yang)液，使(shi)培(pei)養(yang)液溫(wen)度(du)與室溫(wen)一 致。1分鐘(zhong)內融(rong)化細胞(bao)(禁止(zhi)搖晃(huang)凍(dong)存管(guan))，移(yi)取至(zhi)試管(guan)內，動作要(yao)輕，從試管(guan)管(guan)底開始(shi)慢慢上提(ti) 移(yi)液管(guan)，使(shi)細胞(bao)在培(pei)養(yang)液內分布(bu)均勻，離心(xin)，洗2次。

相關熱賣產(chan)品：

其它(ta)經驗概括：

細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)型(xing)(xing)(xing)根(gen)據是否附(fu)于支(zhi)持(chi)物(wu)上(shang)(shang)(shang)生(sheng)(sheng)長(chang)的特性，分(fen)(fen)為(wei)(wei) 貼(tie)附(fu)型(xing)(xing)(xing)、懸浮(fu)型(xing)(xing)(xing)。 貼(tie)附(fu)型(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)附(fu)在(zai)(zai)(zai)(zai)支(zhi)持(chi)物(wu)表面(mian)生(sheng)(sheng)長(chang)，只依賴貼(tie)附(fu)才能生(sheng)(sheng)長(chang)的細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做(zuo)貼(tie)附(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種(zhong)(zhong)現(xian)象(xiang)與(yu)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)(fen)化(hua)有(you)關(guan)。 貼(tie)附(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的分(fen)(fen)型(xing)(xing)(xing) 1.成(cheng)纖(xian)維型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來自中(zhong)(zhong)胚(pei)層(ceng) 特點：與(yu)體內成(cheng)纖(xian)維細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)態(tai)相(xiang)似(si),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體梭型(xing)(xing)(xing)或(huo)不(bu)(bu)(bu)規(gui)(gui)(gui)則三角(jiao)形(xing)(xing),中(zhong)(zhong)央有(you)圓(yuan)形(xing)(xing)核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)向外伸(shen)出(chu)(chu)2～3個長(chang)短不(bu)(bu)(bu)同的突起。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)生(sheng)(sheng)長(chang)時(shi)呈(cheng)放(fang)射狀(zhuang),漩渦或(huo)火焰狀(zhuang)走行。 起源(yuan)(yuan)：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來自中(zhong)(zhong)胚(pei)層(ceng)間(jian)(jian)(jian)充質(zhi)(zhi)組(zu)織。 除真正的成(cheng)纖(xian)維細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌、平滑肌、成(cheng)骨(gu)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)管內皮(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)培(pei)養(yang)時(shi)成(cheng)為(wei)(wei)成(cheng)纖(xian)維型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一種(zhong)(zhong)慣稱(cheng),與(yu)體內細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)同。 2.上(shang)(shang)(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來自外胚(pei)層(ceng)。 特點：扁平不(bu)(bu)(bu)規(gui)(gui)(gui)則多(duo)(duo)角(jiao)型(xing)(xing)(xing)、圓(yuan)形(xing)(xing)核(he)、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密相(xiang)連(lian)成(cheng)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)殖(zhi)(zhi)數目增(zeng)多(duo)(duo)時(shi),整個上(shang)(shang)(shang)皮(pi)膜(mo)隨之移動。邊(bian)緣細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活動“ 拉(la)網(wang)”現(xian)象(xiang)與(yu)起源(yuan)(yuan)內外胚(pei)層(ceng)組(zu)織有(you)關(guan)。 組(zu)織: 皮(pi)膚(fu)表皮(pi)及其(qi)(qi)衍(yan)生(sheng)(sheng)物(wu)(汗(han)腺、皮(pi)脂腺)消化(hua)道分(fen)(fen)上(shang)(shang)(shang)皮(pi)、肝、胰、肺(fei)泡上(shang)(shang)(shang)皮(pi)。 3. 游走型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點：在(zai)(zai)(zai)(zai)支(zhi)持(chi)物(wu)上(shang)(shang)(shang)散(san)在(zai)(zai)(zai)(zai)生(sheng)(sheng)長(chang),不(bu)(bu)(bu)連(lian)接成(cheng)片,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)伸(shen)出(chu)(chu)偽足或(huo)突起呈(cheng)活躍(yue)的游走或(huo)變形(xing)(xing)運(yun)動,速度(du)快不(bu)(bu)(bu)規(gui)(gui)(gui)則, 密度(du)大連(lian)接成(cheng)片呈(cheng)多(duo)(duo)角(jiao)形(xing)(xing)不(bu)(bu)(bu)易(yi)與(yu)其(qi)(qi)他類(lei)型(xing)(xing)(xing)的細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別。 4. 多(duo)(duo)形(xing)(xing)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點： 無規(gui)(gui)(gui)律的形(xing)(xing)態(tai)，如(ru)神經(jing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮(fu)型(xing)(xing)(xing) 特點： 不(bu)(bu)(bu)貼(tie)附(fu)在(zai)(zai)(zai)(zai)支(zhi)持(chi)物(wu)生(sheng)(sheng)長(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體圓(yuan)形(xing)(xing)，在(zai)(zai)(zai)(zai)培(pei)養(yang)液中(zhong)(zhong)生(sheng)(sheng)長(chang)空間(jian)(jian)(jian)大，可(ke)長(chang)時(shi)間(jian)(jian)(jian)的生(sheng)(sheng)長(chang)，繁殖(zhi)(zhi)旺盛(sheng)便(bian)于做(zuo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝(xie)研究。S180肉瘤、血(xue)液里(li)的白細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)(fen)型(xing)(xing)(xing)的目的： 方便(bian)描(miao)述細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)培(pei)養(yang)過程中(zhong)(zhong)的形(xing)(xing)態(tai)變化(hua)。培(pei)養(yang)條件好時(shi)，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相(xiang)對穩定，可(ke)反(fan)映出(chu)(chu)起源(yuan)(yuan)、正常異常的區(qu)別，作(zuo)為(wei)(wei)判定細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)物(wu)學性狀(zhuang)指(zhi)標(biao)。 強調：一般形(xing)(xing)態(tai)并不(bu)(bu)(bu)是一項可(ke)靠指(zhi)標(biao)要(yao)受各方面(mian)影響(xiang)。 如(ru)：反(fan)復開關(guan)溫箱、溫度(du)、C02的濃度(du)、培(pei)養(yang)基變堿、支(zhi)原(yuan)體、pH值。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)接種(zhong)(zhong)時(shi)呈(cheng)三角(jiao)型(xing)(xing)(xing)狀(zhuang)態(tai)，經(jing)培(pei)養(yang)一定時(shi)間(jian)(jian)(jian)后(hou)，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)傳代前(qian)已形(xing)(xing)成(cheng)上(shang)(shang)(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

KG-1a細(xi)胞(bao)，人急(ji)性(xing)骨(gu)髓白血病(bing)細(xi)胞(bao)      25T

MV4-11細胞，人急性白血病細胞    25T

HL-60細胞，人急性早幼粒白血病細胞    25T

U937細胞(bao)，人急性早幼粒白血病細胞(bao)     25T

MOLM-13細胞，人急性髓系白血病細胞    25T

MOLM-14細(xi)胞，人急性髓(sui)系白血病細(xi)胞    25T

K562細(xi)胞(bao)，人慢性髓系白血病細(xi)胞(bao)  25T

HL-60細(xi)胞，人急(ji)性早幼粒白血(xue)病細(xi)胞    25T

CCRF-CEM細(xi)胞，人(ren)急性淋巴細(xi)胞白(bai)血(xue)病T淋巴細(xi)胞  25T

HS 505.T細(xi)胞(bao)，慢(man)性(xing)淋巴細(xi)胞(bao)白血病(bing)（CLL）細(xi)胞(bao)   25T

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