Spc-a-1 肺腺(xian)癌(ai)細胞(bao)慧穎生物一站式(shi)采購(gou)商(shang)城，細(xi)胞(bao)株細(xi)胞(bao)系，耐藥株，STR鑒定，各品(pin)牌進口胎牛血清(qing)，培(pei)養基(ji)，胰(yi)酶，雙抗，*培(pei)養基(ji)，PBS緩(huan)沖液，DMSO，細(xi)胞(bao)生長因(yin)子等，種類齊全，*！

細(xi)(xi)胞(bao)培養技(ji)(ji)術(shu)屬于(yu)細(xi)(xi)胞(bao)工程范疇，細(xi)(xi)胞(bao)工程包括細(xi)(xi)胞(bao)融合技(ji)(ji)術(shu)、單(dan)克(ke)隆抗體的制備、核移(yi)植技(ji)(ji)術(shu)（克(ke)隆）等,而這些(xie)技(ji)(ji)術(shu)中都有細(xi)(xi)胞(bao)培養過程,所以(yi)說細(xi)(xi)胞(bao)培養是生物(wu)學研究的zui基(ji)本技(ji)(ji)術(shu)之一。

上海(hai)慧穎生(sheng)物供應Spc-a-1 肺腺(xian)癌細胞，活性好、存活率高(gao)。隨著細胞(bao)生物學尤其是干細胞(bao)研究的(de)快速發展，在(zai)細胞(bao)治療領域已(yi)經(jing)進行了大(da)量的(de)探(tan)索，取(qu)得了一系(xi)(xi)(xi)列(lie)的(de)令人振奮的(de)成果，且部(bu)分基礎研究成果已(yi)經(jing)在(zai)心血管系(xi)(xi)(xi)統(tong)疾(ji)(ji)病(bing)(bing) 、神經(jing)系(xi)(xi)(xi)統(tong)疾(ji)(ji)病(bing)(bing)、肌肉(rou)骨(gu)骼相關疾(ji)(ji)病(bing)(bing)、糖尿病(bing)(bing)等。

養細(xi)胞經驗分享：

1.細(xi)胞(bao)形態(tai)觀察(cha)。細(xi)胞(bao)膜是(shi)每天必(bi)須(xu)觀察(cha)的(de)，看是(shi)否清晰，細(xi)胞(bao)的(de)死亡往往是(shi)從細(xi)胞(bao)膜的(de)破裂開始的(de)，早期的(de)表現就(jiu)是(shi)細(xi)胞(bao)膜某(mou)一點出現欠(qian)完(wan)整或者毛發影。

2.細(xi)胞往往會(hui)有“抱團”現象，抱團生長或死亡(wang)，個人認為(wei)應(ying)該勤換液或傳代，盡(jin)量避 免(mian)細(xi)胞抱團。

3.細(xi)胞生長迅速，強烈建議用(yong)較大的培養瓶養，用(yong)小瓶養的話，換液不(bu)及時，很容(rong)易死(si) 亡。細(xi)胞的生長速度(du)一(yi)般為(wei)2天(tian)左右就需要傳(chuan)代，并且一(yi)般為(wei)一(yi)傳(chuan)三(san)，或一(yi)傳(chuan)四。

4.培養液：1640|DMEM|MEM|IMDM|a-MEM培養液，1L一般加碳酸氫鈉2g，并加雙(shuang)抗，胎(tai)牛血清濃(nong)度(du)*為12%，zui低(di)不(bu)低(di)于10%，有(you)些(xie)細(xi)胞對(dui)胎(tai)牛血清高度(du)依賴(lai)，比如WRO細(xi)胞，HT-22細(xi)胞，HA-VSMC細胞(bao)，NK-92細胞(bao)等(deng)。培養(yang)液PH應調為7.20-7.40之間(jian)。

5.防污(wu)(wu)染(ran)。L-02細胞生長迅速，較少(shao)發生污(wu)(wu)染(ran)，但常規措施應(ying)該做好。比如無菌操作，酒(jiu)精 消毒等(deng)，培(pei)養瓶(ping)口建(jian)議過火(huo)，包括瓶(ping)蓋，應(ying)過火(huo)4秒左右。

6.凍(dong)存(cun)(cun)。-20一小時，-80次夜(ye)，液(ye)氮(dan)長期(qi)保(bao)存(cun)(cun)。強烈建議液(ye)氮(dan)長期(qi)保(bao)存(cun)(cun)，盡量(liang)不要在(zai)-80長期(qi) 保(bao)存(cun)(cun)，死亡率很(hen)高。

7.復蘇。調節水浴箱(xiang)溫度為42℃，并提(ti)前在(zai)試管內(nei)(nei)放置10倍(bei)培(pei)養(yang)液(ye)(ye)，使(shi)培(pei)養(yang)液(ye)(ye)溫度與室溫一 致。1分(fen)鐘(zhong)內(nei)(nei)融化細胞(禁止搖晃凍(dong)存管)，移取(qu)至試管內(nei)(nei)，動作要輕，從(cong)試管管底(di)開(kai)始慢(man)慢(man)上提(ti) 移液(ye)(ye)管，使(shi)細胞在(zai)培(pei)養(yang)液(ye)(ye)內(nei)(nei)分(fen)布均(jun)勻，離(li)心，洗2次。

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其它經驗概括：

細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing)根(gen)據(ju)是否(fou)附(fu)(fu)(fu)(fu)(fu)于支持物(wu)上(shang)(shang)(shang)生(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)特(te)性，分(fen)為(wei) 貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)、懸浮型(xing)(xing)(xing)(xing)(xing)。 貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)在(zai)(zai)(zai)(zai)(zai)支持物(wu)表(biao)面(mian)生(sheng)長(chang)(chang)，只(zhi)依賴貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)才(cai)能生(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象與(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化有關(guan)。 貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)分(fen)型(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)纖維(wei)(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自中胚層 特(te)點(dian)(dian)：與(yu)體內(nei)(nei)成(cheng)(cheng)纖維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)態(tai)(tai)相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體梭型(xing)(xing)(xing)(xing)(xing)或(huo)不(bu)(bu)規則(ze)三角形(xing)(xing),中央有圓(yuan)形(xing)(xing)核,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質向外伸出(chu)2～3個長(chang)(chang)短不(bu)(bu)同的(de)(de)(de)(de)(de)突(tu)起。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)(zai)生(sheng)長(chang)(chang)時(shi)呈(cheng)放射狀,漩渦或(huo)火焰狀走(zou)行。 起源：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自中胚層間充質組織。 除真正的(de)(de)(de)(de)(de)成(cheng)(cheng)纖維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心(xin)肌、平滑肌、成(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管內(nei)(nei)皮(pi)(pi)(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)(zai)培養(yang)(yang)時(shi)成(cheng)(cheng)為(wei)成(cheng)(cheng)纖維(wei)(wei)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一(yi)種慣稱,與(yu)體內(nei)(nei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)同。 2.上(shang)(shang)(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自外胚層。 特(te)點(dian)(dian)：扁(bian)平不(bu)(bu)規則(ze)多角型(xing)(xing)(xing)(xing)(xing)、圓(yuan)形(xing)(xing)核、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相連成(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖數(shu)目增多時(shi),整(zheng)個上(shang)(shang)(shang)皮(pi)(pi)(pi)膜(mo)隨之移動。邊緣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而(er)單獨活動“ 拉網”現象與(yu)起源內(nei)(nei)外胚層組織有關(guan)。 組織: 皮(pi)(pi)(pi)膚表(biao)皮(pi)(pi)(pi)及其衍生(sheng)物(wu)(汗(han)腺(xian)、皮(pi)(pi)(pi)脂腺(xian))消化道(dao)分(fen)上(shang)(shang)(shang)皮(pi)(pi)(pi)、肝、胰、肺泡上(shang)(shang)(shang)皮(pi)(pi)(pi)。 3. 游(you)走(zou)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點(dian)(dian)：在(zai)(zai)(zai)(zai)(zai)支持物(wu)上(shang)(shang)(shang)散在(zai)(zai)(zai)(zai)(zai)生(sheng)長(chang)(chang),不(bu)(bu)連接成(cheng)(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質伸出(chu)偽足(zu)或(huo)突(tu)起呈(cheng)活躍(yue)的(de)(de)(de)(de)(de)游(you)走(zou)或(huo)變(bian)形(xing)(xing)運(yun)動,速度快不(bu)(bu)規則(ze), 密(mi)度大(da)連接成(cheng)(cheng)片(pian)呈(cheng)多角形(xing)(xing)不(bu)(bu)易(yi)與(yu)其他類型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別(bie)。 4. 多形(xing)(xing)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點(dian)(dian)： 無規律的(de)(de)(de)(de)(de)形(xing)(xing)態(tai)(tai)，如(ru)神(shen)經(jing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮型(xing)(xing)(xing)(xing)(xing) 特(te)點(dian)(dian)： 不(bu)(bu)貼(tie)(tie)附(fu)(fu)(fu)(fu)(fu)在(zai)(zai)(zai)(zai)(zai)支持物(wu)生(sheng)長(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體圓(yuan)形(xing)(xing)，在(zai)(zai)(zai)(zai)(zai)培養(yang)(yang)液(ye)中生(sheng)長(chang)(chang)空間大(da)，可(ke)長(chang)(chang)時(shi)間的(de)(de)(de)(de)(de)生(sheng)長(chang)(chang)，繁殖旺盛便于做細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝研究。S180肉瘤、血液(ye)里的(de)(de)(de)(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)： 方便描述細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)(zai)培養(yang)(yang)過程中的(de)(de)(de)(de)(de)形(xing)(xing)態(tai)(tai)變(bian)化。培養(yang)(yang)條件(jian)好時(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩(wen)定(ding)，可(ke)反(fan)映(ying)出(chu)起源、正常異常的(de)(de)(de)(de)(de)區別(bie)，作為(wei)判(pan)定(ding)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)物(wu)學(xue)性狀指(zhi)標。 強調：一(yi)般形(xing)(xing)態(tai)(tai)并不(bu)(bu)是一(yi)項(xiang)可(ke)靠指(zhi)標要受各方面(mian)影響。 如(ru)：反(fan)復開關(guan)溫箱、溫度、C02的(de)(de)(de)(de)(de)濃度、培養(yang)(yang)基變(bian)堿、支原體、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)(zai)接種時(shi)呈(cheng)三角型(xing)(xing)(xing)(xing)(xing)狀態(tai)(tai)，經(jing)培養(yang)(yang)一(yi)定(ding)時(shi)間后，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)(zai)(zai)傳代(dai)前已形(xing)(xing)成(cheng)(cheng)上(shang)(shang)(shang)皮(pi)(pi)(pi)型(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

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