MCA-RH7777鼠肝癌細胞，進口胎牛血清(qing)慧穎生物一站式采購商城，細(xi)(xi)胞(bao)株細(xi)(xi)胞(bao)系，耐藥株，STR鑒定，各品牌進口胎牛血清(qing)，培養基，胰酶(mei)，雙(shuang)抗，*培養基，PBS緩沖液，DMSO，細(xi)(xi)胞(bao)生長(chang)因子等，種類齊全，*！

細(xi)胞(bao)培(pei)養技(ji)(ji)術屬(shu)于(yu)細(xi)胞(bao)工(gong)程范疇，細(xi)胞(bao)工(gong)程包括(kuo)細(xi)胞(bao)融合技(ji)(ji)術、單克(ke)隆抗(kang)體的(de)制備、核移植技(ji)(ji)術（克(ke)隆）等(deng),而(er)這些(xie)技(ji)(ji)術中(zhong)都有細(xi)胞(bao)培(pei)養過程,所以說(shuo)細(xi)胞(bao)培(pei)養是生物學研(yan)究(jiu)的(de)zui基本技(ji)(ji)術之一。

上海慧穎生(sheng)物供應MCA-RH7777鼠肝癌細胞(bao)，進口胎牛(niu)血清，活性好、存活率高。隨著細胞生物學尤其是干細胞研(yan)究的(de)快速發展，在細胞治(zhi)療領(ling)域已經(jing)進行了(le)大量的(de)探索(suo)，取得了(le)一系(xi)列的(de)令(ling)人振奮的(de)成果，且部分基礎研(yan)究成果已經(jing)在心血管系(xi)統疾病 、神經(jing)系(xi)統疾病、肌肉(rou)骨骼相(xiang)關疾病、糖尿(niao)病等。

養(yang)細(xi)胞經驗分享：

1.細(xi)胞形態(tai)觀察。細(xi)胞膜(mo)是(shi)每天(tian)必(bi)須觀察的(de)，看是(shi)否清(qing)晰，細(xi)胞的(de)死亡(wang)往(wang)(wang)往(wang)(wang)是(shi)從細(xi)胞膜(mo)的(de)破裂開始的(de)，早(zao)期的(de)表現就是(shi)細(xi)胞膜(mo)某一(yi)點出現欠完整或者毛發(fa)影。

2.細(xi)胞(bao)往(wang)往(wang)會有“抱團(tuan)(tuan)”現象，抱團(tuan)(tuan)生長或(huo)死亡，個人認為應該勤(qin)換液或(huo)傳代(dai)，盡量避 免細(xi)胞(bao)抱團(tuan)(tuan)。

3.細胞生(sheng)長迅速，強烈建議用(yong)(yong)較大的培養瓶養，用(yong)(yong)小瓶養的話，換液(ye)不及時，很容易(yi)死 亡(wang)。細胞的生(sheng)長速度(du)一般為2天左右就(jiu)需要(yao)傳代，并且(qie)一般為一傳三，或(huo)一傳四。

4.培養(yang)(yang)液：1640|DMEM|MEM|IMDM|a-MEM培養(yang)(yang)液，1L一般加碳酸(suan)氫(qing)鈉(na)2g，并加雙抗，胎牛血清(qing)濃度*為12%，zui低不(bu)低于(yu)10%，有些細胞對(dui)胎牛血清(qing)高度依賴(lai)，比如WRO細胞，HT-22細胞，HA-VSMC細(xi)胞，NK-92細(xi)胞等。培養液(ye)PH應(ying)調為7.20-7.40之間。

5.防污(wu)染(ran)。L-02細胞生長迅速(su)，較少發(fa)生污(wu)染(ran)，但常規措施應該做好。比如無(wu)菌(jun)操作，酒精(jing) 消毒等，培養瓶口建(jian)議過(guo)(guo)火，包括瓶蓋(gai)，應過(guo)(guo)火4秒左右。

6.凍存。-20一小時，-80次(ci)夜，液(ye)氮長(chang)(chang)期保存。強烈建(jian)議液(ye)氮長(chang)(chang)期保存，盡量不要在(zai)-80長(chang)(chang)期 保存，死亡率很高。

7.復蘇。調節水(shui)浴箱溫度(du)為(wei)42℃，并提前在(zai)試(shi)(shi)管(guan)內(nei)放置(zhi)10倍培養(yang)液，使(shi)培養(yang)液溫度(du)與室溫一 致。1分鐘內(nei)融(rong)化細胞(bao)(禁止搖(yao)晃(huang)凍存管(guan))，移取至試(shi)(shi)管(guan)內(nei)，動作(zuo)要輕，從試(shi)(shi)管(guan)管(guan)底開始慢慢上(shang)提 移液管(guan)，使(shi)細胞(bao)在(zai)培養(yang)液內(nei)分布均勻，離(li)心，洗(xi)2次。

相關熱賣(mai)產品：

其它經(jing)驗概括：

細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)(lei)型(xing)(xing)(xing) 編(bian)輯 細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)類(lei)(lei)型(xing)(xing)(xing)根據是否附(fu)(fu)(fu)于(yu)(yu)支(zhi)持物(wu)(wu)上(shang)(shang)生(sheng)長(chang)(chang)(chang)的(de)(de)特性，分(fen)為 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)、懸浮(fu)型(xing)(xing)(xing)。 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)(fu)(fu)在(zai)(zai)(zai)支(zhi)持物(wu)(wu)表面(mian)生(sheng)長(chang)(chang)(chang)，只(zhi)依(yi)賴貼(tie)(tie)附(fu)(fu)(fu)才能生(sheng)長(chang)(chang)(chang)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫(jiao)做貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象與細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化(hua)有(you)關。 貼(tie)(tie)附(fu)(fu)(fu)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)分(fen)型(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自中(zhong)胚(pei)層 特點(dian)(dian)：與體(ti)內(nei)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)態(tai)(tai)(tai)相(xiang)似(si),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)梭型(xing)(xing)(xing)或(huo)不規則(ze)三(san)(san)角(jiao)(jiao)(jiao)形(xing)(xing)(xing)(xing),中(zhong)央有(you)圓(yuan)形(xing)(xing)(xing)(xing)核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質向外(wai)伸出(chu)2～3個(ge)長(chang)(chang)(chang)短不同(tong)的(de)(de)突(tu)起(qi)(qi)。細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)生(sheng)長(chang)(chang)(chang)時(shi)(shi)呈放射狀(zhuang),漩渦(wo)或(huo)火焰狀(zhuang)走行。 起(qi)(qi)源(yuan)：細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自中(zhong)胚(pei)層間充質組織(zhi)(zhi)。 除真正的(de)(de)成(cheng)(cheng)(cheng)(cheng)纖維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌、平滑肌、成(cheng)(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血管(guan)內(nei)皮(pi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)培(pei)(pei)養時(shi)(shi)成(cheng)(cheng)(cheng)(cheng)為成(cheng)(cheng)(cheng)(cheng)纖維(wei)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)是一種慣稱(cheng),與體(ti)內(nei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不同(tong)。 2.上(shang)(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自外(wai)胚(pei)層。 特點(dian)(dian)：扁平不規則(ze)多角(jiao)(jiao)(jiao)型(xing)(xing)(xing)、圓(yuan)形(xing)(xing)(xing)(xing)核(he)、細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊密(mi)相(xiang)連成(cheng)(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖數目增多時(shi)(shi),整(zheng)個(ge)上(shang)(shang)皮(pi)膜隨之移動。邊緣(yuan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)很(hen)少脫離(li)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨活動“ 拉網”現象與起(qi)(qi)源(yuan)內(nei)外(wai)胚(pei)層組織(zhi)(zhi)有(you)關。 組織(zhi)(zhi): 皮(pi)膚表皮(pi)及其(qi)(qi)衍生(sheng)物(wu)(wu)(汗腺、皮(pi)脂腺)消化(hua)道分(fen)上(shang)(shang)皮(pi)、肝(gan)、胰、肺泡上(shang)(shang)皮(pi)。 3. 游(you)走型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特點(dian)(dian)：在(zai)(zai)(zai)支(zhi)持物(wu)(wu)上(shang)(shang)散在(zai)(zai)(zai)生(sheng)長(chang)(chang)(chang),不連接(jie)(jie)成(cheng)(cheng)(cheng)(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)質伸出(chu)偽足(zu)或(huo)突(tu)起(qi)(qi)呈活躍的(de)(de)游(you)走或(huo)變形(xing)(xing)(xing)(xing)運(yun)動,速度快不規則(ze), 密(mi)度大連接(jie)(jie)成(cheng)(cheng)(cheng)(cheng)片(pian)呈多角(jiao)(jiao)(jiao)形(xing)(xing)(xing)(xing)不易(yi)與其(qi)(qi)他類(lei)(lei)型(xing)(xing)(xing)的(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區別。 4. 多形(xing)(xing)(xing)(xing)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特點(dian)(dian)： 無(wu)規律的(de)(de)形(xing)(xing)(xing)(xing)態(tai)(tai)(tai)，如神(shen)經(jing)(jing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮(fu)型(xing)(xing)(xing) 特點(dian)(dian)： 不貼(tie)(tie)附(fu)(fu)(fu)在(zai)(zai)(zai)支(zhi)持物(wu)(wu)生(sheng)長(chang)(chang)(chang)，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)體(ti)圓(yuan)形(xing)(xing)(xing)(xing)，在(zai)(zai)(zai)培(pei)(pei)養液中(zhong)生(sheng)長(chang)(chang)(chang)空(kong)間大，可長(chang)(chang)(chang)時(shi)(shi)間的(de)(de)生(sheng)長(chang)(chang)(chang)，繁殖旺盛便于(yu)(yu)做細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究。S180肉瘤、血液里(li)的(de)(de)白細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)的(de)(de)目的(de)(de)： 方便描述細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)培(pei)(pei)養過(guo)程中(zhong)的(de)(de)形(xing)(xing)(xing)(xing)態(tai)(tai)(tai)變化(hua)。培(pei)(pei)養條件好時(shi)(shi)，細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)相(xiang)對穩定(ding)，可反映(ying)出(chu)起(qi)(qi)源(yuan)、正常(chang)異常(chang)的(de)(de)區別，作(zuo)為判(pan)定(ding)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)物(wu)(wu)學性狀(zhuang)指標。 強(qiang)調：一般形(xing)(xing)(xing)(xing)態(tai)(tai)(tai)并不是一項(xiang)可靠指標要受各方面(mian)影響。 如：反復開關溫箱、溫度、C02的(de)(de)濃(nong)度、培(pei)(pei)養基變堿(jian)、支(zhi)原體(ti)、pH值。細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)接(jie)(jie)種時(shi)(shi)呈三(san)(san)角(jiao)(jiao)(jiao)型(xing)(xing)(xing)狀(zhuang)態(tai)(tai)(tai)，經(jing)(jing)培(pei)(pei)養一定(ding)時(shi)(shi)間后，細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)(zai)傳代前已形(xing)(xing)(xing)(xing)成(cheng)(cheng)(cheng)(cheng)上(shang)(shang)皮(pi)型(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

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