SGC-7901/VCR 耐長春新堿胃腺癌細胞慧(hui)穎生物(wu)一站式采購商城，細胞(bao)株(zhu)細胞(bao)系(xi)，耐藥(yao)株(zhu)，STR鑒定，各品(pin)牌進口胎(tai)牛血清，培養基，胰酶，雙抗(kang)，*培養基，PBS緩(huan)沖液，DMSO，細胞(bao)生長因子(zi)等(deng)，種(zhong)類齊全，*！

細(xi)(xi)胞(bao)培(pei)養技(ji)(ji)術(shu)(shu)屬于細(xi)(xi)胞(bao)工程范疇，細(xi)(xi)胞(bao)工程包括(kuo)細(xi)(xi)胞(bao)融(rong)合技(ji)(ji)術(shu)(shu)、單克隆(long)抗體的制備、核(he)移植(zhi)技(ji)(ji)術(shu)(shu)（克隆(long)）等,而(er)這些(xie)技(ji)(ji)術(shu)(shu)中(zhong)都有細(xi)(xi)胞(bao)培(pei)養過程,所以說(shuo)細(xi)(xi)胞(bao)培(pei)養是生物學研究的zui基本(ben)技(ji)(ji)術(shu)(shu)之一(yi)。

上(shang)海慧穎生物供應SGC-7901/VCR 耐(nai)長春新堿胃(wei)腺癌細(xi)胞，活(huo)性好、存活(huo)率高。隨著細胞(bao)生(sheng)物學尤其是干細胞(bao)研究的(de)快速發(fa)展，在(zai)細胞(bao)治療領域已(yi)經進行了(le)大量的(de)探索，取得了(le)一系(xi)列的(de)令人振奮的(de)成果(guo)，且部分(fen)基(ji)礎研究成果(guo)已(yi)經在(zai)心血管系(xi)統(tong)疾病(bing) 、神經系(xi)統(tong)疾病(bing)、肌肉(rou)骨骼相關疾病(bing)、糖尿(niao)病(bing)等。

養(yang)細胞經驗分享：

1.細(xi)(xi)胞形態觀(guan)察(cha)。細(xi)(xi)胞膜是每天必(bi)須觀(guan)察(cha)的(de)，看是否清晰，細(xi)(xi)胞的(de)死(si)亡(wang)往(wang)往(wang)是從細(xi)(xi)胞膜的(de)破裂(lie)開始的(de)，早期的(de)表現就(jiu)是細(xi)(xi)胞膜某(mou)一點出現欠完(wan)整(zheng)或者毛發(fa)影。

2.細胞往往會有“抱團”現(xian)象，抱團生長或死(si)亡，個人(ren)認為應(ying)該勤換液或傳代，盡量避 免細胞抱團。

3.細胞(bao)生長迅速，強烈建議用(yong)較大的(de)培(pei)養(yang)瓶養(yang)，用(yong)小瓶養(yang)的(de)話，換液不及時，很容易死 亡。細胞(bao)的(de)生長速度(du)一(yi)般(ban)為2天左(zuo)右就需要傳(chuan)代，并且一(yi)般(ban)為一(yi)傳(chuan)三(san)，或一(yi)傳(chuan)四。

4.培(pei)養液(ye)：1640|DMEM|MEM|IMDM|a-MEM培(pei)養液(ye)，1L一(yi)般(ban)加碳酸氫鈉2g，并加雙抗(kang)，胎牛血清濃度(du)(du)*為12%，zui低不低于10%，有些細(xi)胞(bao)(bao)(bao)對胎牛血清高度(du)(du)依賴，比如WRO細(xi)胞(bao)(bao)(bao)，HT-22細(xi)胞(bao)(bao)(bao)，HA-VSMC細胞，NK-92細胞等(deng)。培(pei)養液(ye)PH應調為7.20-7.40之間。

5.防污(wu)染。L-02細胞生(sheng)長迅速，較少發(fa)生(sheng)污(wu)染，但(dan)常規(gui)措(cuo)施應該做(zuo)好。比如無菌操作，酒(jiu)精 消毒等，培養瓶口建(jian)議(yi)過火，包括瓶蓋，應過火4秒左右。

6.凍存(cun)。-20一小(xiao)時(shi)，-80次夜，液氮(dan)長期(qi)保存(cun)。強烈建議液氮(dan)長期(qi)保存(cun)，盡量不(bu)要在-80長期(qi) 保存(cun)，死亡率很高。

7.復蘇。調節水浴箱溫度為(wei)42℃，并提前在(zai)試管(guan)內(nei)(nei)放置10倍培(pei)養液，使(shi)培(pei)養液溫度與室溫一 致。1分(fen)鐘內(nei)(nei)融化細胞(bao)(禁止搖晃凍(dong)存(cun)管(guan))，移取至試管(guan)內(nei)(nei)，動作(zuo)要輕，從試管(guan)管(guan)底開始慢(man)慢(man)上提 移液管(guan)，使(shi)細胞(bao)在(zai)培(pei)養液內(nei)(nei)分(fen)布均勻，離心，洗(xi)2次。

相關(guan)熱賣產品：

其(qi)它經驗(yan)概括：

細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing)(xing)(xing) 編輯 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)類型(xing)(xing)(xing)(xing)(xing)(xing)(xing)根據(ju)是(shi)否附(fu)(fu)于支持(chi)物(wu)上(shang)生(sheng)(sheng)長的(de)(de)特(te)性(xing)，分(fen)為(wei) 貼(tie)(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)、懸浮(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)。 貼(tie)(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing) 細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)附(fu)(fu)在(zai)(zai)支持(chi)物(wu)表面(mian)(mian)生(sheng)(sheng)長，只(zhi)依賴(lai)貼(tie)(tie)附(fu)(fu)才(cai)能生(sheng)(sheng)長的(de)(de)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)叫做(zuo)(zuo)貼(tie)(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(Anchorrage-dependent cells)這種現象(xiang)(xiang)與細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化有關(guan)(guan)。 貼(tie)(tie)附(fu)(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)分(fen)型(xing)(xing)(xing)(xing)(xing)(xing)(xing) 1.成(cheng)(cheng)(cheng)纖(xian)維(wei)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)：(fibroblast) 來(lai)自(zi)中胚層 特(te)點(dian)：與體內(nei)成(cheng)(cheng)(cheng)纖(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)形(xing)(xing)(xing)(xing)(xing)(xing)(xing)態(tai)相似,胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體梭(suo)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)或不(bu)(bu)規(gui)則三角(jiao)形(xing)(xing)(xing)(xing)(xing)(xing)(xing),中央有圓形(xing)(xing)(xing)(xing)(xing)(xing)(xing)核(he),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)向外(wai)伸(shen)出2～3個(ge)長短不(bu)(bu)同的(de)(de)突(tu)起(qi)(qi)。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)生(sheng)(sheng)長時(shi)呈放射狀(zhuang)(zhuang)(zhuang),漩渦(wo)或火(huo)焰狀(zhuang)(zhuang)(zhuang)走行。 起(qi)(qi)源(yuan)(yuan)(yuan)：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)來(lai)自(zi)中胚層間充質(zhi)(zhi)組織(zhi)。 除真正的(de)(de)成(cheng)(cheng)(cheng)纖(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、心肌(ji)、平(ping)滑(hua)肌(ji)、成(cheng)(cheng)(cheng)骨細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、血(xue)(xue)管內(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 注意：細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培(pei)(pei)養(yang)時(shi)成(cheng)(cheng)(cheng)為(wei)成(cheng)(cheng)(cheng)纖(xian)維(wei)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)是(shi)一種慣稱(cheng),與體內(nei)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)同。 2.上(shang)皮(pi)(pi)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(epithlium cell type) 來(lai)自(zi)外(wai)胚層。 特(te)點(dian)：扁平(ping)不(bu)(bu)規(gui)則多角(jiao)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)、圓形(xing)(xing)(xing)(xing)(xing)(xing)(xing)核(he)、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)緊(jin)密相連成(cheng)(cheng)(cheng)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)增殖(zhi)數目(mu)增多時(shi),整個(ge)上(shang)皮(pi)(pi)膜(mo)隨(sui)之移動(dong)。邊緣(yuan)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)很少脫離細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)群而單獨(du)活(huo)動(dong)“ 拉網”現象(xiang)(xiang)與起(qi)(qi)源(yuan)(yuan)(yuan)內(nei)外(wai)胚層組織(zhi)有關(guan)(guan)。 組織(zhi): 皮(pi)(pi)膚表皮(pi)(pi)及(ji)其(qi)衍生(sheng)(sheng)物(wu)(汗腺、皮(pi)(pi)脂腺)消化道(dao)分(fen)上(shang)皮(pi)(pi)、肝、胰、肺泡上(shang)皮(pi)(pi)。 3. 游(you)走型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(wandering cell type) 特(te)點(dian)：在(zai)(zai)支持(chi)物(wu)上(shang)散在(zai)(zai)生(sheng)(sheng)長,不(bu)(bu)連接(jie)成(cheng)(cheng)(cheng)片(pian),胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)質(zhi)(zhi)伸(shen)出偽足或突(tu)起(qi)(qi)呈活(huo)躍的(de)(de)游(you)走或變形(xing)(xing)(xing)(xing)(xing)(xing)(xing)運(yun)動(dong),速度(du)(du)快(kuai)不(bu)(bu)規(gui)則, 密度(du)(du)大連接(jie)成(cheng)(cheng)(cheng)片(pian)呈多角(jiao)形(xing)(xing)(xing)(xing)(xing)(xing)(xing)不(bu)(bu)易與其(qi)他類型(xing)(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao) 區(qu)別。 4. 多形(xing)(xing)(xing)(xing)(xing)(xing)(xing)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(polymorphic cell type) 特(te)點(dian)： 無規(gui)律的(de)(de)形(xing)(xing)(xing)(xing)(xing)(xing)(xing)態(tai)，如(ru)神(shen)經細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。 懸浮(fu)型(xing)(xing)(xing)(xing)(xing)(xing)(xing) 特(te)點(dian)： 不(bu)(bu)貼(tie)(tie)附(fu)(fu)在(zai)(zai)支持(chi)物(wu)生(sheng)(sheng)長，胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)體圓形(xing)(xing)(xing)(xing)(xing)(xing)(xing)，在(zai)(zai)培(pei)(pei)養(yang)液中生(sheng)(sheng)長空間大，可(ke)長時(shi)間的(de)(de)生(sheng)(sheng)長，繁殖(zhi)旺盛(sheng)便于做(zuo)(zuo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝研究(jiu)。S180肉瘤、血(xue)(xue)液里的(de)(de)白細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、K562、 HL－60。 分(fen)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)目(mu)的(de)(de)： 方便描述細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)培(pei)(pei)養(yang)過程中的(de)(de)形(xing)(xing)(xing)(xing)(xing)(xing)(xing)態(tai)變化。培(pei)(pei)養(yang)條件好時(shi)，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)相對穩定，可(ke)反映出起(qi)(qi)源(yuan)(yuan)(yuan)、正常(chang)異(yi)常(chang)的(de)(de)區(qu)別，作為(wei)判(pan)定細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)物(wu)學性(xing)狀(zhuang)(zhuang)(zhuang)指(zhi)標(biao)。 強調：一般形(xing)(xing)(xing)(xing)(xing)(xing)(xing)態(tai)并不(bu)(bu)是(shi)一項可(ke)靠指(zhi)標(biao)要受(shou)各方面(mian)(mian)影響。 如(ru)：反復開(kai)關(guan)(guan)溫(wen)箱、溫(wen)度(du)(du)、C02的(de)(de)濃度(du)(du)、培(pei)(pei)養(yang)基變堿、支原體、pH值。細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)接(jie)種時(shi)呈三角(jiao)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)狀(zhuang)(zhuang)(zhuang)態(tai)，經培(pei)(pei)養(yang)一定時(shi)間后，細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在(zai)(zai)傳代前已形(xing)(xing)(xing)(xing)(xing)(xing)(xing)成(cheng)(cheng)(cheng)上(shang)皮(pi)(pi)型(xing)(xing)(xing)(xing)(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)。

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U937細(xi)胞(bao)，人急性(xing)早幼粒白(bai)血病細(xi)胞(bao)  ;   25T

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K562細(xi)胞，人慢性(xing)髓系白血病(bing)細(xi)胞  25T

HL-60細(xi)胞(bao)，人急性早幼(you)粒白血病(bing)細(xi)胞(bao)    25T

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