聯系電話:
潮霉素B(Hygromycin B)Roche老包裝,大(da)量庫存(cun),*,訂購: 56980388 !
產品名稱:潮(chao)霉素B
英文(wen)名稱(cheng):hygromycin B
品牌:Roche 老包裝(zhuang)
貨號:
規格:1G
市場價格:1933元
定義:潮(chao)霉素(su)B由吸水鏈(lian)霉菌(Streptomyces hygroscopicus)產生(sheng)的(de)一種氨基糖(tang)苷類(lei)抗生(sheng)素(su),通過抑(yi)制蛋白質合成能(neng)殺死(si)細菌、真(zhen)菌和高(gao)等真(zhen)核細胞,用于篩選和保(bao)持轉化(hua)有潮(chao)霉素(su)抗性基因的(de)原(yuan)核和真(zhen)核細胞。
用(yong)途:
1)潮霉(mei)素B是氨基糖苷(gan)類抗生(sheng)素,它可以通過(guo)抑(yi)制蛋白質合成殺死細菌(jun)、真菌(jun)和(he)高(gao)級真核細胞。
2)潮霉素B通過干(gan)擾(rao)移(yi)位、促(cu)進(jin)80S核糖體的(de)誤譯來抑制蛋白質合(he)成。
3)哺乳動物(wu)細胞選擇的有效濃(nong)度(du)(du)通常是50 μg/ml--1 mg/ml之間;對(dui)于(yu)植物(wu)細胞,其有效濃(nong)度(du)(du)是20–200 μg/ml;對(dui)于(yu)細菌,有效濃(nong)度(du)(du)是20–200μg/ml;對(dui)于(yu)真菌,有效濃(nong)度(du)(du)是200μg–1 mg/ml。
4)轉染中(zhong)用的特定抗生素。
5)潮霉素B磷酸(suan)轉移(yi)酶滅活潮霉素B。
6)攜(xie)帶hph基因(yin)轉染的細(xi)胞對(dui)潮霉素有抗(kang)性(xing)。
7)轉(zhuan)染細(xi)胞穩定或暫時(shi)表達該基因。
產品描述:
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
Quality
Purity: >80% (HPLC)