聯系電話:
潮(chao)霉素B(Hygromycin B)Roche新(xin)包(bao)裝,大量庫存,*,訂購: 56980388 !
產品(pin)名稱:潮霉素B
英文名稱:hygromycin B
品牌:Roche 新(xin)包(bao)裝(zhuang) 推(tui)薦(jian)
貨號(hao):
規格:1G
市場(chang)價格:1933元
定義:潮霉(mei)(mei)素(su)(su)B由吸(xi)水鏈霉(mei)(mei)菌(jun)(Streptomyces hygroscopicus)產生的一種氨基(ji)糖苷類抗生素(su)(su),通(tong)過(guo)抑制蛋白質合(he)成能殺死細(xi)菌(jun)、真菌(jun)和高等真核(he)細(xi)胞,用于(yu)篩選(xuan)和保(bao)持(chi)轉(zhuan)化有(you)潮霉(mei)(mei)素(su)(su)抗性基(ji)因的原核(he)和真核(he)細(xi)胞。
用途:
1)潮(chao)霉(mei)素B是氨基糖苷類抗(kang)生素,它可以通過(guo)抑制蛋白質合成殺(sha)死細菌(jun)(jun)、真(zhen)菌(jun)(jun)和(he)高級真(zhen)核細胞。
2)潮(chao)霉(mei)素(su)B通過干擾移位、促進80S核(he)糖(tang)體(ti)的誤譯來抑制蛋白質合成。
3)哺乳動物(wu)細(xi)胞選擇的有效(xiao)濃度(du)(du)通常是(shi)50 μg/ml--1 mg/ml之間;對(dui)于(yu)植物(wu)細(xi)胞,其有效(xiao)濃度(du)(du)是(shi)20–200 μg/ml;對(dui)于(yu)細(xi)菌,有效(xiao)濃度(du)(du)是(shi)20–200μg/ml;對(dui)于(yu)真菌,有效(xiao)濃度(du)(du)是(shi)200μg–1 mg/ml。
4)轉染中用的特(te)定抗生素(su)。
5)潮霉素B磷酸轉移酶滅活潮霉素B。
6)攜帶hph基因轉染的(de)細胞對潮霉素有抗性。
7)轉(zhuan)染細胞(bao)穩定或(huo)暫時表(biao)達該基因。
產品描述:
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
Quality
Purity: >80% (HPLC)