*CC趨化因子受體2人CCR-2高品質ELISA試劑盒，慧穎訂購： 56980388！

產(chan)品名(ming)稱：人(ren)CC趨化因子受體2（CCR-2）ELISA試劑盒

規格：48T/96T

試劑(ji)(ji)盒特點(dian)：采用純進(jin)口試劑(ji)(ji)和(he)進(jin)口工藝流程，質量穩定，重復性好(hao)，靈(ling)敏度高，特異(yi)性強等優點(dian)，適合客戶人(ren)群。

試劑(ji)盒組成(cheng)：

試劑盒組成(cheng) 48孔配置 96孔配置 保存(cun)

說明(ming)書 1份 1份

封板膜 2片（48） 2片（96）

密封(feng)袋(dai) 1個 1個

酶標(biao)包(bao)被板 1×48 1×96 2-8℃保存(cun)

標準(zhun)品：36ng/mL 0.5ml×1瓶 0.5ml×1瓶 2-8℃保(bao)存

標準品稀釋液 1.5ml×1瓶 1.5ml×1瓶(ping) 2-8℃保存

酶標試(shi)劑 3 ml×1瓶 6 ml×1瓶(ping) 2-8℃保(bao)存

樣(yang)品稀釋液 3 ml×1瓶(ping) 6 ml×1瓶 2-8℃保(bao)存

顯(xian)色劑A液 3 ml×1瓶 6 ml×1瓶 2-8℃保存

顯色劑(ji)B液 3 ml×1瓶(ping) 6 ml×1瓶 2-8℃保(bao)存

終止液 3ml×1瓶 6ml×1瓶 2-8℃保(bao)存(cun)

濃(nong)縮洗滌液 （20ml×20倍）×1瓶 （20ml×30倍）×1瓶 2-8℃保存

操作步驟：

1. 標(biao)(biao)準(zhun)品(pin)(pin)的稀(xi)釋(shi)與加(jia)(jia)(jia)(jia)樣：在(zai)(zai)酶標(biao)(biao)包被板(ban)上(shang)設標(biao)(biao)準(zhun)品(pin)(pin)孔(kong)(kong)10孔(kong)(kong)，在(zai)(zai)*、第(di)(di)(di)(di)二(er)(er)孔(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)100μl，然后在(zai)(zai)*、第(di)(di)(di)(di)二(er)(er)孔(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)稀(xi)釋(shi)液(ye)50μl，混(hun)勻(yun)；然后從*孔(kong)(kong)、第(di)(di)(di)(di)二(er)(er)孔(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)三孔(kong)(kong)和第(di)(di)(di)(di)四孔(kong)(kong)，再在(zai)(zai)第(di)(di)(di)(di)三、第(di)(di)(di)(di)四孔(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)稀(xi)釋(shi)液(ye)50μl，混(hun)勻(yun)；然后在(zai)(zai)第(di)(di)(di)(di)三孔(kong)(kong)和第(di)(di)(di)(di)四孔(kong)(kong)中(zhong)(zhong)(zhong)先各(ge)(ge)(ge)(ge)取(qu)(qu)50μl棄(qi)掉，再各(ge)(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)稀(xi)釋(shi)液(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后從第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)稀(xi)釋(shi)液(ye)50μl，混(hun)勻(yun)后從第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十孔(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)準(zhun)品(pin)(pin)稀(xi)釋(shi)液(ye)50μl，混(hun)勻(yun)后從第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)(qu)50μl棄(qi)掉。（稀(xi)釋(shi)后各(ge)(ge)(ge)(ge)孔(kong)(kong)加(jia)(jia)(jia)(jia)樣量都為(wei)(wei)50μl，濃度分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)為(wei)(wei)24ng/mL，16ng/mL, 8ng/mL，4 ng/mL，2ng/mL）。

2. 加樣：分別設(she)空(kong)白孔(kong)（空(kong)白對(dui)照(zhao)孔(kong)不加樣品及酶(mei)標試(shi)劑，其余各步(bu)操作(zuo)相同）、待(dai)測(ce)樣品(pin)(pin)孔。在(zai)酶標包被板上待(dai)測(ce)樣品(pin)(pin)孔中先加樣品(pin)(pin)稀釋(shi)液(ye)40μl，然后再加待(dai)測樣(yang)品(pin)10μl（樣品zui終稀釋度為5倍）。加(jia)樣將樣品加(jia)于酶標板(ban)孔底部，盡(jin)量(liang)不(bu)觸及孔壁，輕(qing)輕(qing)晃動混勻。

3. 溫育：用封板(ban)膜(mo)封板(ban)后置37℃溫(wen)育30分鐘。

4. 配液：將30（48T的20倍(bei)）倍(bei)濃縮洗滌液用蒸餾水30（48T的20倍(bei)）倍(bei)稀釋后備用。

5. 洗滌：小心揭掉封板(ban)膜(mo)，棄(qi)去液(ye)體(ti)(ti)，甩干，每孔加滿(man)洗滌液(ye)，靜置30秒后棄(qi)去，如此重(zhong)復5次，拍干。

6. 加(jia)(jia)酶：每孔加(jia)(jia)入(ru)酶標試劑(ji)50μl，空白孔除(chu)外。

7. 溫育：操(cao)作(zuo)同(tong)3。

8. 洗滌(di)：操(cao)作同5。

9. 顯(xian)色(se)(se)：每孔先加入顯(xian)色(se)(se)劑A50μl，再加入顯(xian)色(se)(se)劑B50μl，輕(qing)(qing)輕(qing)(qing)震(zhen)蕩混勻，37℃避光顯色15分鐘. 

10. 終止(zhi)：每孔加終止(zhi)液50μl，終止(zhi)反(fan)應(ying)（此(ci)時藍色(se)立(li)轉黃色(se)）。

11. 測(ce)定：以空白空調零，450nm波長依(yi)序測(ce)量各孔的(de)吸光度（OD值）。 測(ce)定應在加終止液(ye)后15分鐘以內(nei)進行。

試劑盒檢測(ce)范圍(wei)：0.8 ng/mL -24ng/mL 

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