人MCP-1ELISA試劑盒產品介紹：

產品名稱：人MCP-1ELISA試劑盒；人單核細胞趨化蛋白-1 ELISA試劑盒；人MCP-1ELISA檢測試劑盒；人單核細胞趨化蛋白-1酶免試劑盒

英文名稱：Human MCP-1 ELISA KIT

方法：一步法ELISA/兩步法ELISA

規格：48T/96T

試劑(ji)盒來源(yuan)：采用純進口(kou)試劑(ji)和進口(kou)工藝流程

人MCP-1ELISA試劑盒（兩步法）重復性好、質量穩定、特異性請、靈敏度高等優點

操作步(bu)驟：

1. 標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)的稀(xi)釋與加(jia)(jia)(jia)(jia)(jia)樣：在(zai)(zai)(zai)(zai)酶標(biao)(biao)(biao)包被板上設標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)*、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)100μl，然后(hou)在(zai)(zai)(zai)(zai)*、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)釋液(ye)(ye)50μl，混(hun)勻(yun)(yun)；然后(hou)從*孔(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)100μl分別加(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)分別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)釋液(ye)(ye)50μl，混(hun)勻(yun)(yun)；然后(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先各取(qu)50μl棄掉，再(zai)(zai)各取(qu)50μl分別加(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)釋液(ye)(ye)50ul，混(hun)勻(yun)(yun)；混(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)50μl分別加(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)釋液(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分別取(qu)50μl加(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)分別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)釋液(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)50μl棄掉。（稀(xi)釋后(hou)各孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣量(liang)都為50μl，濃度(du)分別為240ng/L，160ng/L ，80ng/L，40ng/L， 20ng/L）。

2. 加(jia)樣：分(fen)別設空白孔（空白對照孔不加(jia)樣品及酶標試劑，其(qi)余各(ge)步操作相同）、待測(ce)樣(yang)品(pin)(pin)孔。在酶標包被板上待測(ce)樣(yang)品(pin)(pin)孔中先加(jia)樣(yang)品(pin)(pin)稀(xi)釋液40μl，然后再加待測樣(yang)品(pin)10μl（樣品zui終稀釋(shi)度為5倍(bei)）。加樣將樣品(pin)加于酶標(biao)板孔(kong)底部，盡量不觸及孔(kong)壁，輕輕晃動混勻。

3. 溫育：用封(feng)板膜封(feng)板后置(zhi)37℃溫育30分鐘(zhong)。

4. 配(pei)液(ye)(ye)：將30（48T的20倍）倍濃縮洗滌液(ye)(ye)用蒸餾(liu)水30（48T的20倍）倍稀釋后備用。

5. 洗滌：小心揭掉(diao)封板膜，棄去(qu)液體，甩干，每孔加滿洗滌液，靜置30秒后棄去(qu)，如此重復5次，拍干。

6. 加酶(mei)：每孔加入(ru)酶(mei)標試劑50μl，空白孔除外。

7. 溫育：操作(zuo)同3。

8. 洗滌：操(cao)作同5。

9. 顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕(qing)輕(qing)震蕩混勻，37℃避光顯色15分(fen)鐘. 

10. 終止(zhi)：每(mei)孔加終止(zhi)液50μl，終止(zhi)反應（此時(shi)藍色立(li)轉黃色）。

11. 測定：以空(kong)白空(kong)調零(ling)，450nm波長依序測量各(ge)孔(kong)的吸光度（OD值）。 測定應在加終止液(ye)后(hou)15分鐘(zhong)以內進(jin)行。

試(shi)劑盒性(xing)能(neng)：

1.樣品線性回歸與預期濃度(du)相(xiang)關系(xi)數R值為0.92以上。

2.批(pi)內(nei)與(yu)批(pi)見(jian)應分別小于9%和(he)15%

檢測(ce)范(fan)圍：                                              

15ng/L -300ng/L 

人MCP-1ELISA試劑盒（一步法）精準、快速、穩定等優點

操作步驟

1. 從室溫(wen)平衡20min 后的鋁箔(bo)袋中取出所需(xu)板條(tiao)，剩(sheng)余板條(tiao)用自封袋密封放回4℃。

2. 設置標準(zhun)(zhun)品(pin)(pin)孔(kong)和(he)樣本孔(kong)，標準(zhun)(zhun)品(pin)(pin)孔(kong)各加不同濃度的標準(zhun)(zhun)品(pin)(pin)50μL；

3. 樣本孔(kong)先(xian)加待測(ce)樣本10μL，再加樣本稀(xi)釋液40μL；空白孔(kong)不加。

4. 除空白孔外，標準品孔和(he)樣本孔中每孔加入(ru)辣根(gen)過氧化物酶（HRP）標記的檢測抗(kang)體(ti)100μL，用(yong)封板膜封住反應孔，37℃水浴鍋(guo)或恒(heng)溫(wen)箱溫(wen)育60min。

5. 棄去(qu)液(ye)體，吸水紙上(shang)拍干，每孔加(jia)滿洗(xi)(xi)滌液(ye)，靜置1min，甩去(qu)洗(xi)(xi)滌液(ye)，吸水紙上(shang)拍干，如(ru)此重復(fu)洗(xi)(xi)板(ban)5 次（也(ye)可用洗(xi)(xi)板(ban)機洗(xi)(xi)板(ban)）。

6. 每(mei)孔(kong)加(jia)入(ru)底物A、B 各50μL，37℃避(bi)光(guang)孵育(yu)15min。

7. 每孔加(jia)入(ru)終止液(ye)50μL，15min 內，在450nm 波長處測定各(ge)孔的OD 值。

試劑盒性能

1. 準確性：標準品(pin)線(xian)性回歸與預(yu)期濃(nong)度相關系數R 值，大于等于

0.9900。

2. 靈敏度：zui低檢測(ce)濃(nong)度小于1.0 pg/mL。

3. 特異性(xing)：不與其(qi)它可(ke)溶(rong)性(xing)結構類(lei)似(si)物交叉反應。

4. 重復性：板內、板間變異系數均小于15%。

5. 貯藏：2-8℃，避光(guang)防潮保存。

6. 有效期：6個月

高(gao)品質(zhi)人MCP-1ELISA試劑盒 盡在(zai)上海慧穎(ying)生物！