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Invitrogen 28025-013 M-MLV Reverse Transcriptase 簡介(jie)：
產品名稱(cheng)：M-MLV Reverse Transcriptase
貨 號：28025-013
規 格：40,000U
品(pin) 牌(pai)：Invitrogen
國產/進口：進 口
儲(chu)存溫度：-20°C
特惠價(jia)格：致(zhi) 電
庫存狀態：現 貨
銷售商：上海(hai)慧(hui)穎生物科技有限公司
慧穎生物專業供應(ying)Invitrogen 28025-013 M-MLV Reverse Transcriptase，常備現貨，*。主營Life Invitrogen產品(pin)，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試(shi)劑、培養基(ji)及原料試(shi)劑、各類胎牛血(xue)清、馬血(xue)清等，如有(you)需要(yao)，詳詢。
Invitrogen 28025-013 M-MLV Reverse Transcriptase描述(shu)：
MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
Thermostability – Optimal activity at 37°C
Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (6).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C (6).
Invitrogen 28025-013 M-MLV Reverse Transcriptase同(tong)類產品(pin)主要有:
| 貨(huo)號 | 名(ming)稱 | 規格 |
| 14750100 | BLOCKIT ALEXA FLUOR RED OLIGO | 20 UM- 250UL |
| K494500 | BLOCK IT U6 ENTRY VECTOR KIT | 20 RXNS |
| 15630080 | HEPES | 10ml |
| NP0060 | NUPAGE MES BIS-TRIS BFR KIT | COMBO |
| NP0321BOX | NuPAGE? Novex 4-12% Bis-Tris Gel 1.0 mm, 10 Well | 10 gels?box |
| 12355036 | PFX50 DNA POL., 500RXN | 500 RXNS |
| A11180 | CLONEMINER CDNA LIB KIT, VER2 | 5 RXN KIT |
| L150201 | GENERACER SUPERSCRPT TA | 5 CDNA- 50 PCR RXNS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 10928-034 | 含有Platinum? Taq DNA聚合(he)酶的SuperScriptTM II一步法RT-PCR系統 | 25 rxns |
| 10928-042 | 100 rxns | |
| 11904-018 | 用于RT-PCR的SuperScriptTM II*鏈(lian)合成系(xi)統 | 50 rxns |
| 18080-200 | SuperScriptTM III CellsDirect cDNA合(he)成試劑(ji)盒 | 25 rxns |
| 18080-300 | 100 rxns | |
| 18080-400 | SuperScriptTM III*鏈合成SuperMix | 50 rxns |
| 18068-015 | DNase I擴增級 | 100U(1U/ul) |
| 48190-011 | 隨(sui)機引物(wu) | 9 units |
| K210005 | PURELINK PLASMID MIDI 50 | 50 REACTIONS |
| S7563 | SYBR? Green I核酸凝膠(jiao)染料 | 500ul |
| S7567 | 1ml | |
| S7564 | SYBR? Green II RNA凝(ning)膠(jiao)染料(liao) | 500ul |
| S7568 | 1ml | |
| 11811031 | GENETICIN | 5G |
| 10459014 | DMRIE-C REAGENT | 1ml |
| 12309019 | OPTI PRO SFM | 1000ml |
| 11514015 | PLUS REAGENT | 0.85 ML |
| 12651014 | FREESTYLE CHO EXPRESSION MEDIU | 1000ML |
| 12338018 | FREESTYLE 293 EXPRESSION MED | 1000ML |
| 12347019 | 293FECTIN | 1ml |
| 16447100 | FreeStyle MAX | 1 ml |
| R25001 | ZEOCIN | 1g |
| 15224-017 | T4 DNA 連(lian)接酶 | 100U(1U/ul) |
| 15224-025 | 500U(1U/ul) | |
| 15224-090 | 4x500U(1U/ul) | |
| 15224-041 | 250U(5U/ul) | |
| 18047-019 | DNase I | 20,000U |
| 18068-015 | DNase 擴增級 | 100U |
| 18021-014 | 核糖(tang)核酸酶(mei)H | 30U(2U/ul) |
| 18021-071 | 4x30U(2U/ul) | |
| 18162-016 | DNA聚合酶I/DNase I | 250U |
| 18005-017 | T4 DNA 聚(ju)合酶 | 50U(5U/ul) |
| 18005-025 | 250U(5U/ul) | |
| 25530-015 | 蛋白酶K | 100mg |
| 25530-031 | 1g | |
| 18374-058 | 5`RACE系統， | 10 rxns |
| 18373-019 | 3`RACE系統， | 20 rxns |
| MPK10096 | Neon? Transfection System ;100 μL Kit | |
| K280020 | ZERO BLUNT TOPO PCRCLON KIT | 20 RXN |
| 18012021 | LARGE FRAGMENT | 100 UN |
| 18021014 | RNASE H (E.COLI) | 30 UN |
| 4311816 | AmpliTaq Gold? DNA Polymerase, 1000 units with Gold Buffer and MgCl2 solution | 1000 un |
| 18092015 | CDNA SIZE FRAC COLUMNS | 3 COLUMNS |
| 450512 | BLOCK-IT H1 RNAI VECTOR KIT | 20 RXNS |
| 10511012 | 1 kb DNA Extension Ladder | 100 UG |
Invitrogen產品(pin)(pin)種(zhong)類(lei)較多，本展(zhan)臺無法一一體現，以(yi)上是部分(fen)產品(pin)(pin)，若有需要的產品(pin)(pin)，請及(ji)時與我們取得，咨詢。
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