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Invitrogen 12328019 CLONED AMV RT, 750U簡介:
產品名稱:Invitrogen 12328019 CLONED AMV RT, 750U
貨 號:12328019
規 格:750 units
品 牌:Invitrogen
分 類:反轉錄酶
國產/進口:進 口
儲存溫度:-20°C
特惠價格:致 電
庫存狀態:現 貨
銷售商:上海慧穎生物科技有限公司
慧穎生物專業供應Invitrogen 12328019 CLONED AMV RT, 750U,常備現貨,*。主營Life Invitrogen產品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試劑、培養基及原料試劑、各類胎牛血清、馬血清等,如有需要,詳詢。
Invitrogen 12328019 CLONED AMV RT, 750U描述:
AMV Reverse Transcriptase, Cloned, is used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA. Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb. AMV RT has endoribonuclease H activity.
Features of this enzyme:
? Flexibility—works with oligo(dT), random primers, or gene-specific primers
? Thermostability—AMV is active at temperatures up to 55°C
? Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA
Source
Purified from insect cells infected with recombinant baculovirus expressing AMV pol.
Unit definition
One unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer.
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