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產品名稱：Invitrogen 18012021 LARGE FRAGMENT
貨 號：18012021
規 格：100 UN
分(fen) 類(lei)：DNA/RNA 聚合酶(mei)
品 牌：Invitrogen
國(guo)產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀(zhuang)態：現 貨(huo)
銷(xiao)售商：上海慧穎生物科技有限公(gong)司
慧穎生物專(zhuan)業供應Invitrogen 18012021 LARGE FRAGMENT，常備現貨，*。主營(ying)Life Invitrogen產(chan)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑(ji)、凝膠填料(liao)、常規化學試劑(ji)、培養基及原料(liao)試劑(ji)、各類胎牛(niu)血清、馬血清等，如有需要，詳詢。
描述：
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit ;the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.
Applications:
Fill-in of 5′ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template?primer.
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