庫存現貨：HTR-8細胞，人滋養細胞，說明書/價格 全國各地均可發貨，全國統一價格

產(chan)品名稱：HTR-8細(xi)胞

中文(wen)名稱：人(ren)滋養細胞

形態特(te)性：上皮樣

生長特性(xing)：貼壁(bi)生長

培養(yang)條件(jian)：DMEM/F12+15%FBS

傳代方法：1：2傳代

凍(dong)存條件：90%FBS+10%dmso

庫(ku)存(cun)狀(zhuang)態：現(xian)貨

細胞(bao)純度：94％

細胞(bao)活力：90％（ViabilitybyTrypanBlueExclusion）

細胞(bao)檢測：細胞(bao)不含有HIV-1、HBV、HCV、支原體(ti)、細菌、酵母和(he)真菌

慧穎生(sheng)物提供(gong)(gong)400多種(zhong)細(xi)(xi)胞(bao)(bao)(bao)系細(xi)(xi)胞(bao)(bao)(bao)株(zhu),以及相(xiang)應的(de)細(xi)(xi)胞(bao)(bao)(bao)學(xue)實驗服務:血管新生(sheng)、traswell、劃(hua)痕實驗、細(xi)(xi)胞(bao)(bao)(bao)遷移、克(ke)隆形成、細(xi)(xi)胞(bao)(bao)(bao)凋亡、細(xi)(xi)胞(bao)(bao)(bao)增殖(zhi)、細(xi)(xi)胞(bao)(bao)(bao)周期等。另有(you)：示蹤細(xi)(xi)胞(bao)(bao)(bao)、穩定細(xi)(xi)胞(bao)(bao)(bao)株(zhu)構建、細(xi)(xi)胞(bao)(bao)(bao)代感(gan)染、篩藥細(xi)(xi)胞(bao)(bao)(bao)株(zhu)產品、篩藥細(xi)(xi)胞(bao)(bao)(bao)株(zhu)定制、pathway研(yan)究細(xi)(xi)胞(bao)(bao)(bao)、STR分型鑒定，細(xi)(xi)胞(bao)(bao)(bao)染色等技術服務提供(gong)(gong)。咨(zi)詢(xun)： ！

HTR-8細(xi)(xi)胞(bao)(bao)，人(ren)滋養細(xi)(xi)胞(bao)(bao)，說明(ming)書(shu)/價格 細胞傳代培養技術：

細胞復蘇

1. 將凍存的(de)細(xi)胞取出后迅速放入37℃水浴(yu)中，并不時搖動，在一分鐘內使其*融化

2.將細胞吸出放入(ru)(ru)有培(pei)養(yang)(yang)基(ji)(ji)的離(li)心管中(zhong)中(zhong)，用滴管輕輕吹打混(hun)(hun)(hun)勻，800轉/min，5分鐘離(li)心；棄去上清液，加入(ru)(ru)培(pei)養(yang)(yang)基(ji)(ji)，混(hun)(hun)(hun)勻再離(li)心一(yi)次，棄液，加入(ru)(ru)4~5ml 培(pei)養(yang)(yang)基(ji)(ji)，混(hun)(hun)(hun)勻，移(yi)入(ru)(ru)培(pei)養(yang)(yang)瓶中(zhong)

細胞換液

1.用舊(jiu)液體輕輕沖(chong)洗培養(yang)瓶底，然后用滴管吸盡舊(jiu)液（棄去）

2.加入PBS1~2ml，輕(qing)輕(qing)搖(yao)晃(huang)，然后用滴(di)管吸盡舊液(ye)（棄去）

3.如(ru)果細胞液(ye)比較臟，可以重復步驟2

4.加入(ru)新培(pei)(pei)養基(ji)，輕輕搖勻，放入(ru)培(pei)(pei)養箱繼續(xu)培(pei)(pei)養

細胞傳代

1.用舊(jiu)液體輕(qing)輕(qing)沖洗(xi)培養瓶底，然后用滴管(guan)吸(xi)盡舊(jiu)液（棄去(qu)）

2.加入PBS1~2ml，輕(qing)輕(qing)搖晃，然后用滴管吸盡舊液（棄去）

3.加入約1~1.5ml胰酶，室(shi)溫(wen)或培養箱中放置4~5分(fen)鐘，直(zhi)到所有貼(tie)壁(bi)細胞消(xiao)化(hua)下(xia)來為止【判斷(duan)方(fang)法：倒置相差顯微鏡觀察(cha)，細胞變圓(yuan)時就可以終止消(xiao)化(hua)】

4.加入約1ml培養基（含有小(xiao)牛(niu)血清(qing)）終止(zhi)消化

5.用滴管吹洗一次，移入離心(xin)管, 800轉(zhuan)/min，5分鐘離心(xin)

6.加(jia)入培養(yang)(yang)基，吹打分散(san)細胞(bao)(bao)，再根(gen)據分傳細胞(bao)(bao)瓶數補加(jia)一(yi)定量的(de)含(han)血清的(de)培養(yang)(yang)基，制(zhi)成細胞(bao)(bao)懸液；分裝至2~3個培養(yang)(yang)瓶中

7.放入培養箱進行(xing)培養

細胞(bao)計數(shu)

1.準備好顯(xian)微鏡、載(zai)玻片、蓋玻片、血細胞計(ji)數板、吸管等儀器設(she)備

2.按照“細胞(bao)傳代”中講述的方法(fa)制成細胞(bao)懸液

3.將細(xi)胞懸液取(qu)出若干(gan)，滴入血(xue)細(xi)胞計數板，注意不要有氣(qi)泡進入

4.加樣后靜置2~3min后觀察，數(shu)四角大格內的細胞總數(shu)。計(ji)數(shu)時(shi)應按照一定的路徑(jing)，以免遺漏或(huo)重復。細胞壓(ya)線時(shi)計(ji)上不計(ji)下，計(ji)左(zuo)不計(ji)右，計(ji)數(shu)2~3次，取平(ping)均值

5. 細(xi)胞(bao)密度 = 四大格中(zhong)細(xi)胞(bao)總(zong)數÷4 ×104 = 細(xi)胞(bao)數/ml懸液

細胞凍存

1. 重復“細(xi)胞傳代”中的(de)消化細(xi)胞的(de)步驟，將(jiang)細(xi)胞消化下來，轉移至離(li)心管800轉/min，5分鐘離(li)心，棄液

2. 沉淀中加入凍(dong)存液，輕吹(chui)制成懸液

3. 分(fen)轉(zhuan)到凍存管內，每個1~1.5ml

4. 將(jiang)凍(dong)存(cun)管口封嚴

5.貼(tie)上標簽，注明(ming)凍存時間(jian)、細胞(bao)種類及代數

6. 按下(xia)列順序降溫，并zui終凍存(cun)(cun)：室(shi)溫→ 4℃ 20min → -20℃ 30min →-70℃凍存(cun)(cun)

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Abcam Anti-Nanog抗體[EPR2027(2)] (ab109250) 100UL

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Abcam Anti-c-Kit抗體(ti)[YR145] (ab32363)   100UL

CST ABCG2 (D5V2K) XP® Rabbit mAb #42078 100UL

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Abcam Anti-Sp7 / Osterix抗體(ti)(ti)- ChIP Grade (ab22552)  50UG

人趨化(hua)性(xing)細胞(bao)因子(zi)CCL20 ELISA試劑盒  48T/96T

人粒(li)細胞(bao)-巨噬(shi)細胞(bao)集落(luo)刺(ci)激因子(zi)(GM-GSF)ELISA試劑(ji)盒    48T/96T

人白介素(su)-17A(IL-17A)ELISA試劑盒(he) 48T/96T

人白介素17B(IL17B)ELISA試(shi)劑盒  48T/96T

慧穎生物關于出售的HTR-8細(xi)胞，人滋(zi)養(yang)細(xi)胞，說明書/價(jia)格污染問題，請在收到產品48小時內，給我們提出真實的實驗結果；細胞活性問題，請在收到產品7天內給我們提出真實的實驗結果，我們調查核實后予免費調換，不收取任何費用。

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主推產品三(san)：細(xi)胞株(zhu)細(xi)胞系

400多(duo)種類細(xi)胞(bao)株細(xi)胞(bao)系(xi)，20多(duo)株耐藥(yao)株，公司擁有(you)完善(shan)的細(xi)胞(bao)庫管理體系(xi)，80%細(xi)胞(bao)技術(shu)人(ren)員為(wei)碩士學歷，二十多(duo)年的細(xi)胞(bao)培(pei)養經驗，所(suo)售細(xi)胞(bao)背景清(qing)晰、傳代次數少(shao)、*、售后一對一服務，跟蹤及時到位(wei)。

熱賣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)：DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Ishikawa細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW116細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。

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