庫存現貨：SMMC-7721細(xi)胞，人(ren)肝癌(ai)細(xi)胞株(zhu)，價格/說明書 全國各地均可發貨，全國統一價格

產品名稱：SMMC-7721細(xi)胞(bao)

中文名(ming)稱：人肝癌細(xi)胞

形(xing)態特性(xing)：上皮樣

生長(chang)特性：貼壁生長(chang)

培(pei)養基(ji)：DMEM 90%, Fetal Bovine Serum 10%

培養條件：37℃ 5% CO2

消(xiao)化條件：0.25% (w/v) Trypsin-0.53 mM EDTA溶液，消(xiao)化5-10min

傳化比例：1：2-1：4

換(huan)液時間：2-3天換(huan)液一次

凍存液(ye)比(bi)例：85%培養基，10%FBS, 5% (v/v) DMSO

凍存密度(du)：1-3 ×10^6/管(guan)，液氮保存細胞純度(du)：94％

細胞活力：90％（ViabilitybyTrypanBlueExclusion）

細(xi)胞(bao)檢(jian)測：細(xi)胞(bao)不含有HIV-1、HBV、HCV、支原體、細(xi)菌(jun)、酵母和真菌(jun)

慧(hui)穎(ying)生(sheng)物(wu)提供400多種細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)系(xi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu),以及相(xiang)應的細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)學實(shi)驗(yan)服務:血管新生(sheng)、traswell、劃痕實(shi)驗(yan)、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)遷移、克隆形成、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凋亡(wang)、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增殖、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周期(qi)等(deng)。另有：示蹤細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、穩(wen)定細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)構建、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)代感染、篩(shai)藥(yao)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)產(chan)品、篩(shai)藥(yao)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)定制、pathway研(yan)究細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、STR分型鑒定，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)染色等(deng)技術服務提供。咨詢： ！

SMMC-7721細(xi)胞(bao)，人肝癌細(xi)胞(bao)株(zhu)，價格/說明書(shu) 細胞(bao)傳代培養技術：

細胞(bao)復蘇

1. 將凍存的細胞取(qu)出后(hou)迅速放入(ru)37℃水浴中，并不時搖(yao)動，在(zai)一分(fen)鐘內(nei)使其*融化

2.將細(xi)胞吸出放入(ru)(ru)有培養(yang)基的離心(xin)管(guan)中中，用(yong)滴管(guan)輕(qing)(qing)輕(qing)(qing)吹打混(hun)勻(yun)，800轉/min，5分鐘離心(xin)；棄(qi)去上清液(ye)，加入(ru)(ru)培養(yang)基，混(hun)勻(yun)再離心(xin)一(yi)次(ci)，棄(qi)液(ye)，加入(ru)(ru)4~5ml 培養(yang)基，混(hun)勻(yun)，移入(ru)(ru)培養(yang)瓶中

細胞換液(ye)

1.用(yong)舊(jiu)液體輕(qing)輕(qing)沖洗(xi)培養瓶底，然后用(yong)滴管吸盡舊(jiu)液（棄去）

2.加入(ru)PBS1~2ml，輕(qing)輕(qing)搖晃，然后用(yong)滴管吸盡舊液（棄去(qu)）

3.如(ru)果細胞液比較(jiao)臟，可以重(zhong)復步驟2

4.加入新培養基(ji)，輕輕搖勻，放入培養箱繼(ji)續培養

細胞傳代

1.用舊(jiu)液(ye)體輕輕沖洗(xi)培(pei)養(yang)瓶(ping)底，然后(hou)用滴(di)管吸盡舊(jiu)液(ye)（棄去）

2.加入PBS1~2ml，輕輕搖晃(huang)，然(ran)后用滴管吸盡舊液（棄去）

3.加入(ru)約1~1.5ml胰酶，室溫(wen)或培(pei)養箱中放置(zhi)4~5分鐘，直到所有(you)貼壁細(xi)胞消化(hua)下(xia)來(lai)為止【判斷方法：倒置(zhi)相差顯微(wei)鏡觀察，細(xi)胞變圓時就(jiu)可以終(zhong)止消化(hua)】

4.加入約1ml培養基(ji)（含有小牛血清(qing)）終止消(xiao)化

5.用滴管(guan)吹洗一次(ci)，移(yi)入(ru)離(li)心管(guan), 800轉/min，5分鐘離(li)心

6.加(jia)入培養基(ji)(ji)，吹打(da)分(fen)散細(xi)胞(bao)(bao)，再根據分(fen)傳細(xi)胞(bao)(bao)瓶數補加(jia)一定量的(de)含血清(qing)的(de)培養基(ji)(ji)，制成(cheng)細(xi)胞(bao)(bao)懸液；分(fen)裝至2~3個培養瓶中

7.放入培(pei)養箱進行培(pei)養

細胞計數

1.準備好顯微鏡(jing)、載(zai)玻片、蓋玻片、血細胞計數板、吸管(guan)等(deng)儀器設備

2.按(an)照“細胞傳代(dai)”中(zhong)講述的方法制成細胞懸液

3.將細胞(bao)懸液(ye)取出若干，滴入血細胞(bao)計(ji)數板，注(zhu)意(yi)不要有氣泡進入

4.加樣后(hou)靜置2~3min后(hou)觀察，數(shu)(shu)四角大格內的細(xi)(xi)胞(bao)總數(shu)(shu)。計(ji)數(shu)(shu)時(shi)應按照一定的路徑，以免遺(yi)漏(lou)或重復。細(xi)(xi)胞(bao)壓線(xian)時(shi)計(ji)上(shang)不計(ji)下，計(ji)左不計(ji)右(you)，計(ji)數(shu)(shu)2~3次(ci)，取平均值

5. 細胞密度 = 四(si)大(da)格中細胞總數(shu)÷4 ×104 = 細胞數(shu)/ml懸液

細(xi)胞凍存

1. 重復“細(xi)(xi)胞傳(chuan)代”中的消化細(xi)(xi)胞的步驟，將(jiang)細(xi)(xi)胞消化下來，轉移至離心(xin)管800轉/min，5分鐘離心(xin)，棄液

2. 沉淀中(zhong)加入凍存液，輕吹制成(cheng)懸液

3. 分轉到(dao)凍存管內，每個1~1.5ml

4. 將凍存管口封嚴

5.貼上標(biao)簽，注明凍存時間、細胞(bao)種類及代數

6. 按(an)下列順序(xu)降溫，并zui終(zhong)凍存：室溫→ 4℃ 20min → -20℃ 30min →-70℃凍存

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慧穎生物關于出售的SMMC-7721細(xi)胞，人肝癌細(xi)胞株(zhu)，價格(ge)/說明(ming)書污染問題，請在收到產品48小時內，給我們提出真實的實驗結果；細胞活性問題，請在收到產品7天內給我們提出真實的實驗結果，我們調查核實后予免費調換，不收取任何費用。

需要客戶(hu)提供細(xi)胞(bao)培養說明(ming)、細(xi)胞(bao)操作處理方法(fa)、細(xi)胞(bao)質量(liang)問題反饋表;如果搞不清原因的(de)，或者(zhe)客戶(hu)直(zhi)接(jie)愿(yuan)意支付(fu)購買價半價的(de)費(fei)用(yong)直(zhi)接(jie)復蘇。

如用戶收到細胞8-30天之內，因處理不(bu)當造成細胞死亡或污(wu)染，我公司將以五折優惠價重新提(ti)供(gong)一株原細胞。

慧(hui)穎生(sheng)(sheng)物科技(ji)（上海(hai)）有限公司(si)位于嘉定區南翔眾仁(ren)路(lu)399號運通星財富廣場，由海(hai)外(wai)華僑和多名中(zhong)科院博士組成的(de)技(ji)術(shu)團(tuan)隊，主(zhu)要致力于免疫學、分子生(sheng)(sheng)物學、病(bing)理、病(bing)理分子生(sheng)(sheng)物、代謝組學、生(sheng)(sheng)物化(hua)學技(ji)術(shu)在科研(yan)(yan)中(zhong)的(de)應用(yong)與推(tui)廣，專業從事相(xiang)關產(chan)品的(de)引進、服務和研(yan)(yan)發(fa)。通過高度細分、*的(de)服務平(ping)臺，向廣大客戶(hu)提供醫學科研(yan)(yan)樣(yang)本檢測(ce)、合作(zuo)研(yan)(yan)究(jiu)、開發(fa)及生(sheng)(sheng)產(chan)。

主推(tui)產品一：ELISA試劑盒

1、專業(ye)的(de)ELISA試劑盒(he)生產企業(ye)

2、*抗(kang)體----高效、靈敏、特異

3、規(gui)范包被(bei)操(cao)作----吸(xi)(xi)附均勻(yun)，吸(xi)(xi)附性好，空白值低(di)，孔底(di)透明度(du)高

4、*的優(you)化方案----重復性高，可靠性強(qiang)

5、購買本公司的ELISA試(shi)劑盒，免費代測(ce)

6、技術服(fu)務要(yao)求(qiu)：專業(ye)，規(gui)范，高(gao)效

7、適(shi)用于體液(ye)、組(zu)織勻漿液(ye)、細胞(bao)培(pei)養上清(qing)液(ye)、尿液(ye)等多(duo)種(zhong)類型的樣本(ben)

8、可檢(jian)測動(dong)物類型豐富：人、猴、大鼠、小(xiao)鼠、豚鼠、黃金地鼠、兔(tu)、豬、犬、牛、綿羊、山羊、鵝、雞、蝦、河(he)蟹、鱸魚、斑馬魚等

9、可檢測(ce)指標齊全：炎癥因子、血(xue)管(guan)生成素(su)、動(dong)脈(mo)粥樣(yang)硬化因子、趨化因子、生長因子基質(zhi)金(jin)屬蛋白酶、脂肪(fang)因子等等

10、經濟(ji)、實惠(hui)、可靠(kao)，完善(shan)、穩定的(de)(de)實驗(yan)體系，優秀的(de)(de)科(ke)研隊伍，*的(de)(de)實驗(yan)設備、準確(que)可靠(kao)的(de)(de)實驗(yan)結果，是(shi)您可靠(kao)的(de)(de)合作伙伴

 我們的(de)優勢：*、完善、穩定(ding)的(de)實驗體(ti)系，優秀的(de)科(ke)研工作者(zhe)，準確可(ke)靠的(de)實驗結果。稟存用(yong)戶(hu)至上的(de)原(yuan)則，竭(jie)誠為你服務。

主(zhu)推產品二：胎牛血清

慧穎(ying)生(sheng)物供(gong)應：胎牛(niu)(niu)血清(qing)(qing)、新(xin)生(sheng)牛(niu)(niu)血清(qing)(qing)、小牛(niu)(niu)血清(qing)(qing)、馬血清(qing)(qing)、山羊血清(qing)(qing)、人血清(qing)(qing)、人AB血清(qing)(qing)、綿羊血清(qing)(qing)、雞血清(qing)(qing)、豬血清(qing)(qing)、封閉驢(lv)血清(qing)(qing)、大鼠血清(qing)(qing)、小鼠血清(qing)(qing)。其中(zhong)特殊胎牛(niu)(niu)血清(qing)(qing)：超低(di)IgG胎牛(niu)(niu)許晴、透(tou)析(xi)血清(qing)(qing)、活(huo)(huo)性(xing)炭處理血清(qing)(qing)以(yi)及活(huo)(huo)性(xing)炭/熱滅活(huo)(huo)血清(qing)(qing)等，庫存常備現貨

囊括主要品牌：德國SERANA、澳洲BOVOGEN、美國GIBCO、美國Hyclone以及(ji)美國SIGMA，熱(re)賣貨號：10099-141、10270-106、SH30084.03、10437-028、SFBS、S-FBS-SA-015、16000-044

慧穎以(yi)每(mei)年5000瓶血清(qing)出庫(ku)到全(quan)國(guo)各地重點實驗室(shi)、高(gao)校、醫(yi)院、制藥單(dan)位(wei)等，目前成為(wei)全(quan)國(guo)幾大動物血清(qing)供應商之一。

主推產(chan)品三(san)：細(xi)胞(bao)株細(xi)胞(bao)系(xi)

400多(duo)種類細胞(bao)株(zhu)細胞(bao)系(xi)，20多(duo)株(zhu)耐藥株(zhu)，公司擁有完善的細胞(bao)庫管(guan)理體系(xi)，80%細胞(bao)技術人員為碩士學歷(li)，二十多(duo)年(nian)的細胞(bao)培(pei)養經(jing)驗，所售(shou)細胞(bao)背景清(qing)晰、傳代(dai)次(ci)數(shu)少(shao)、*、售(shou)后(hou)一對一服務，跟蹤及時到位。

熱賣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)：DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)等。

另有：我司代購美國SANTA CURZ、ABCAM、CST、密理(li)博抗體(ti)，全國格，！