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rMSC細胞rMSC細胞株價格

  • 產品型(xing)號:
  • 產品時間:2024-08-13
  • 簡要描述(shu):rMSC細胞rMSC細胞株價格,慧穎提供rMSC來源,背景資料,細胞形態,培養基,培養條件,細胞詳細說明書以及注意事項等。慧穎細胞庫,擁有完善的細胞庫管理體系,供應400多種類細胞株細胞系:國內/外優質細胞供應,種類齊全,質量保證,,100%放心免費售后!自細胞庫成立至今,供應于全國各省市地區,擁有北京上海各地中科院研究所,復旦,瑞金,上藥所等各大小型院校及企業。
  • 產品簡介

庫存現貨:rMSC細胞(bao)(bao)rMSC細胞(bao)(bao)株(zhu)價格 全國各地均可發貨,全國統一價格

產品名稱:rMSC細胞

中文(wen)名稱:大鼠骨(gu)髓間質干細胞

來源(yuan):大鼠骨(gu)髓間(jian)質(zhi)

形態特性:上(shang)皮樣

生(sheng)長(chang)特(te)性:貼壁(bi)生(sheng)長(chang)

培養(yang)基:DMEM 90%, Fetal Bovine Serum 10%

培養條件:37℃ 5% CO2

消化條件(jian):0.25% (w/v) Trypsin-0.53mM EDTA溶液,消化5-10min

傳化比例:1:2

換(huan)液時間:3天換(huan)液一次

凍存液比例:85%培養基,10%FBS, 5% (v/v) DMSO

凍存密(mi)度:1-2 ×10^6/管,液氮保存細(xi)胞活力(li):90%(ViabilitybyTrypanBlueExclusion)

細胞檢測(ce):細胞不含(han)有HIV-1、HBV、HCV、支原(yuan)體、細菌、酵母(mu)和真(zhen)菌

慧穎生物提(ti)供400多(duo)種細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)系細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)株,以及相應的細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)學實(shi)驗(yan)服務:血管(guan)新生、traswell、劃(hua)痕實(shi)驗(yan)、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)遷移、克隆(long)形成、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凋亡、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)增殖、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)周期等。另有:示蹤細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、穩定細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)株構建、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)代感(gan)染(ran)、篩藥(yao)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)株產品、篩藥(yao)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)株定制、pathway研究細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、STR分(fen)型鑒(jian)定,細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)染(ran)色等技術服務提(ti)供。咨詢: !

rMSC細(xi)(xi)胞rMSC細(xi)(xi)胞株價格 細胞傳代培養技術:

細胞復蘇

1. 將凍存的細胞取出后迅速放入37℃水浴中,并不時搖動,在一分鐘內(nei)使其*融化

2.將(jiang)細胞吸出放入有(you)培(pei)養基的離(li)(li)(li)心(xin)(xin)管中(zhong)(zhong)中(zhong)(zhong),用滴管輕輕吹(chui)打混(hun)勻(yun),800轉/min,5分鐘離(li)(li)(li)心(xin)(xin);棄去上清液,加入培(pei)養基,混(hun)勻(yun)再離(li)(li)(li)心(xin)(xin)一(yi)次,棄液,加入4~5ml 培(pei)養基,混(hun)勻(yun),移入培(pei)養瓶(ping)中(zhong)(zhong)

細胞換液

1.用舊(jiu)(jiu)液體輕輕沖洗培養瓶(ping)底,然后用滴管吸盡舊(jiu)(jiu)液(棄去)

2.加入PBS1~2ml,輕輕搖晃,然后(hou)用(yong)滴管(guan)吸盡舊(jiu)液(棄去)

3.如果細胞(bao)液比較臟,可以重復步驟2

4.加入新培(pei)養基,輕(qing)輕(qing)搖勻,放(fang)入培(pei)養箱繼續培(pei)養

細胞傳代

1.用(yong)舊液體(ti)輕輕沖洗培(pei)養(yang)瓶底,然后用(yong)滴(di)管吸盡舊液(棄去(qu))

2.加(jia)入PBS1~2ml,輕輕搖晃,然后用滴管吸盡舊液(棄去)

3.加入約1~1.5ml胰酶,室溫(wen)或培養(yang)箱中(zhong)放置4~5分(fen)鐘(zhong),直到所(suo)有貼壁細胞消化下(xia)來(lai)為止【判(pan)斷(duan)方法:倒置相差顯微鏡觀(guan)察,細胞變圓時就可以終止消化】

4.加入(ru)約1ml培養基(含有小牛血清)終止消化

5.用滴管吹洗(xi)一次,移入離(li)心管, 800轉/min,5分鐘離(li)心

6.加入培(pei)養(yang)基,吹(chui)打分(fen)(fen)散細(xi)胞,再根(gen)據(ju)分(fen)(fen)傳細(xi)胞瓶數補加一定量的(de)含血清的(de)培(pei)養(yang)基,制(zhi)成細(xi)胞懸(xuan)液;分(fen)(fen)裝至2~3個培(pei)養(yang)瓶中

7.放入培(pei)(pei)養箱進行(xing)培(pei)(pei)養

細胞計數

1.準備好顯微鏡、載玻片、蓋玻片、血細胞計(ji)數板、吸管(guan)等儀(yi)器(qi)設備

2.按照“細胞(bao)傳代”中(zhong)講述的方法制(zhi)成細胞(bao)懸液

3.將細胞(bao)懸液取(qu)出若(ruo)干,滴入(ru)(ru)血細胞(bao)計數板(ban),注(zhu)意不要有氣泡進入(ru)(ru)

4.加樣后靜置2~3min后觀察,數四角大格(ge)內(nei)的(de)細胞總數。計(ji)數時應按照一(yi)定的(de)路徑,以免遺漏或(huo)重復。細胞壓線時計(ji)上不計(ji)下(xia),計(ji)左不計(ji)右,計(ji)數2~3次,取平(ping)均值(zhi)

5. 細胞(bao)(bao)密度 = 四(si)大格(ge)中細胞(bao)(bao)總數(shu)÷4 ×104 = 細胞(bao)(bao)數(shu)/ml懸液

細胞凍存

1. 重復“細(xi)(xi)胞傳代(dai)”中(zhong)的(de)(de)消化(hua)細(xi)(xi)胞的(de)(de)步驟,將細(xi)(xi)胞消化(hua)下(xia)來,轉(zhuan)移至離心管800轉(zhuan)/min,5分鐘離心,棄液

2. 沉(chen)淀中加(jia)入凍(dong)存液,輕吹制成懸液

3. 分轉到凍存管(guan)內,每個1~1.5ml

4. 將凍存管口(kou)封(feng)嚴

5.貼上標簽,注明凍(dong)存時間、細胞(bao)種類及代數

6. 按下列順序降溫,并zui終凍(dong)存:室溫→ 4℃ 20min → -20℃ 30min →-70℃凍(dong)存

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慧穎生物關于出售的rMSC細(xi)胞(bao)rMSC細(xi)胞(bao)株價格 污染問題,請在收到產品48小時內,給我們提出真實的實驗結果;細胞活性問題,請在收到產品7天內給我們提出真實的實驗結果,我們調查核實后予免費調換,不收取任何費用。

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如用戶(hu)收到(dao)細(xi)胞(bao)8-30天之內,因處理不當造成細(xi)胞(bao)死亡或(huo)污(wu)染,我(wo)公司(si)將以五折(zhe)優(you)惠價重新提(ti)供一株原(yuan)細(xi)胞(bao)。

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8、可檢(jian)測動(dong)物類型豐富:人(ren)、猴(hou)、大鼠、小鼠、豚鼠、黃金地鼠、兔、豬、犬、牛、綿羊、山羊、鵝、雞、蝦、河蟹、鱸魚(yu)、斑馬魚(yu)等

9、可(ke)檢測指標(biao)齊全:炎癥因(yin)子(zi)、血管生成(cheng)素、動脈粥樣(yang)硬化因(yin)子(zi)、趨化因(yin)子(zi)、生長因(yin)子(zi)基(ji)質金屬蛋(dan)白酶、脂肪因(yin)子(zi)等(deng)等(deng)

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 我們的(de)(de)(de)(de)優(you)勢:*、完善、穩定的(de)(de)(de)(de)實驗(yan)(yan)體系,優(you)秀的(de)(de)(de)(de)科(ke)研工作者,準確(que)可靠(kao)的(de)(de)(de)(de)實驗(yan)(yan)結果。稟存用戶(hu)至(zhi)上(shang)的(de)(de)(de)(de)原(yuan)則,竭誠為你服務。

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慧穎以每年5000瓶血(xue)清(qing)(qing)出(chu)庫到全國各地重點實驗(yan)室(shi)、高校、醫(yi)院(yuan)、制藥單位等,目前成(cheng)為全國幾大動物血(xue)清(qing)(qing)供應商(shang)之一。

主推產品三:細胞株細胞系

400多種類細胞(bao)株細胞(bao)系(xi),20多株耐藥株,公司擁有完善的細胞(bao)庫管理體(ti)系(xi),80%細胞(bao)技術人員為碩士學歷,二十多年的細胞(bao)培養經驗,所售(shou)細胞(bao)背景清晰、傳(chuan)代(dai)次數少、*、售(shou)后一(yi)對(dui)一(yi)服(fu)務,跟蹤(zong)及(ji)時到位。

熱賣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao):DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。

另有:我司代購(gou)美(mei)國(guo)SANTA CURZ、ABCAM、CST、密理博抗體,全國(guo)格,!

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