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庫存現貨:人乳腺癌細胞株,MDA-MB-231細胞,報價(jia)/說明書 全國各地均可發貨,全國統一價格,運輸形式:復蘇、凍存以及全血清包被(2ML管子)。
產品名稱:MDA-MB-231細胞
中文名稱(cheng):人乳腺癌細胞
來源(yuan):人乳腺癌
形態特(te)性:上(shang)皮(pi)樣
生(sheng)長(chang)(chang)特性:貼壁(bi)生(sheng)長(chang)(chang)
培養(yang)基:DMEM 90%, Fetal Bovine Serum 10%
培(pei)養(yang)條(tiao)件:37℃ 5% CO2
消化條件:0.25% (w/v) Trypsin-0.53mM EDTA溶液,消化5-10min
傳化(hua)比例: 1:2~1:4
換(huan)液時(shi)間:2~3天換(huan)液一次
凍存液比(bi)例 85%培養基,10%FBS, 5% (v/v) DMSO
凍存密度: 1-2 ×10^6/管,液氮保存細胞活(huo)力:90%(ViabilitybyTrypanBlueExclusion)
細(xi)胞檢測(ce):細(xi)胞不(bu)含有HIV-1、HBV、HCV、支原體、細(xi)菌、酵(jiao)母和(he)真菌
慧(hui)穎生物(wu)提供400多種細胞(bao)(bao)系細胞(bao)(bao)株,以及相應的(de)細胞(bao)(bao)學實驗服(fu)務(wu):血管新生、traswell、劃痕實驗、細胞(bao)(bao)遷(qian)移、克(ke)隆形成、細胞(bao)(bao)凋亡(wang)、細胞(bao)(bao)增殖、細胞(bao)(bao)周期(qi)等。另有:示蹤細胞(bao)(bao)、穩定(ding)細胞(bao)(bao)株構(gou)建、細胞(bao)(bao)代感染(ran)、篩藥細胞(bao)(bao)株產品、篩藥細胞(bao)(bao)株定(ding)制、pathway研究細胞(bao)(bao)、STR分(fen)型(xing)鑒定(ding),細胞(bao)(bao)染(ran)色等技術服(fu)務(wu)提供。咨(zi)詢: !
人(ren)乳腺癌細(xi)胞株,MDA-MB-231細(xi)胞,報價/說明書 細胞傳代培養技術:
細胞復(fu)蘇
1. 將凍存的細胞(bao)取出后迅(xun)速放入37℃水浴中,并(bing)不時(shi)搖動,在一分鐘(zhong)內使其*融化
2.將細胞吸出放入有培(pei)(pei)養基的離心管中(zhong)中(zhong),用滴管輕(qing)輕(qing)吹打(da)混(hun)勻,800轉(zhuan)/min,5分鐘離心;棄(qi)去上(shang)清液,加入培(pei)(pei)養基,混(hun)勻再(zai)離心一次(ci),棄(qi)液,加入4~5ml 培(pei)(pei)養基,混(hun)勻,移入培(pei)(pei)養瓶中(zhong)
細(xi)胞換液
1.用舊(jiu)(jiu)液體輕(qing)輕(qing)沖洗培(pei)養瓶底(di),然后用滴管吸盡舊(jiu)(jiu)液(棄去)
2.加入(ru)PBS1~2ml,輕輕搖晃,然后用(yong)滴管(guan)吸(xi)盡舊液(棄去)
3.如果細(xi)胞液比較臟,可以重(zhong)復步驟(zou)2
4.加入新培養(yang)基,輕輕搖勻,放入培養(yang)箱繼續培養(yang)
細胞傳(chuan)代
1.用舊(jiu)液體(ti)輕輕沖洗培(pei)養瓶(ping)底,然(ran)后用滴管吸(xi)盡(jin)舊(jiu)液(棄去)
2.加入(ru)PBS1~2ml,輕輕搖晃,然后(hou)用滴管吸盡(jin)舊液(ye)(棄去)
3.加入約1~1.5ml胰(yi)酶(mei),室溫或培養箱中放置4~5分鐘,直到(dao)所(suo)有貼壁細(xi)胞消化下來為止(zhi)【判斷方法:倒置相差顯微鏡觀察,細(xi)胞變(bian)圓(yuan)時就可以終止(zhi)消化】
4.加入約1ml培養基(含有小牛(niu)血(xue)清)終止消(xiao)化
5.用滴管吹洗一(yi)次(ci),移(yi)入離(li)心(xin)管, 800轉/min,5分鐘離(li)心(xin)
6.加入培(pei)養基,吹打分(fen)(fen)散細胞,再根(gen)據(ju)分(fen)(fen)傳細胞瓶數補加一定量的含血清的培(pei)養基,制成細胞懸(xuan)液;分(fen)(fen)裝至2~3個(ge)培(pei)養瓶中
7.放入培養(yang)箱進行培養(yang)
細胞計數
1.準備好顯微鏡、載玻片、蓋(gai)玻片、血細胞計數板(ban)、吸管等儀器設(she)備
2.按照“細胞傳(chuan)代(dai)”中講(jiang)述的方法(fa)制成細胞懸液(ye)
3.將細(xi)胞懸液取出若(ruo)干,滴(di)入血細(xi)胞計數板,注(zhu)意不要有氣(qi)泡進入
4.加樣后靜置2~3min后觀察,數(shu)四(si)角大格內的(de)細胞總數(shu)。計(ji)(ji)數(shu)時應按照一(yi)定(ding)的(de)路徑,以免遺(yi)漏(lou)或重復。細胞壓線時計(ji)(ji)上不(bu)計(ji)(ji)下,計(ji)(ji)左不(bu)計(ji)(ji)右,計(ji)(ji)數(shu)2~3次,取(qu)平均值
5. 細(xi)胞密度 = 四大(da)格(ge)中細(xi)胞總(zong)數÷4 ×104 = 細(xi)胞數/ml懸(xuan)液
細胞凍存
1. 重(zhong)復“細(xi)胞傳代”中的消化細(xi)胞的步驟,將細(xi)胞消化下來,轉(zhuan)(zhuan)移至離心(xin)管(guan)800轉(zhuan)(zhuan)/min,5分(fen)鐘離心(xin),棄液
2. 沉淀中加入凍(dong)存液,輕吹制(zhi)成懸液
3. 分轉(zhuan)到凍存管(guan)內,每個1~1.5ml
4. 將凍(dong)存管口封(feng)嚴
5.貼上(shang)標簽(qian),注明凍(dong)存時(shi)間、細胞種類及(ji)代數
6. 按下列(lie)順(shun)序降溫,并zui終凍存(cun):室溫→ 4℃ 20min → -20℃ 30min →-70℃凍存(cun)
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需要客(ke)戶(hu)(hu)提供細(xi)胞培養(yang)說(shuo)明(ming)、細(xi)胞操作處理方法、細(xi)胞質量問題反(fan)饋表;如果搞不清(qing)原因的,或者(zhe)客(ke)戶(hu)(hu)直(zhi)接愿(yuan)意支付購買價半價的費用直(zhi)接復(fu)蘇。
如(ru)用戶(hu)收到(dao)細胞8-30天之內,因處(chu)理不當造成細胞死亡或污染,我(wo)公司將以五(wu)折優惠價(jia)重新提(ti)供一(yi)株原細胞。
慧(hui)穎生(sheng)(sheng)物(wu)科(ke)技(ji)(上海(hai))有限公(gong)司位(wei)于嘉定區(qu)南翔眾(zhong)仁路399號運通星財(cai)富廣(guang)場,由海(hai)外華僑和(he)多名中(zhong)科(ke)院(yuan)博士組成的(de)技(ji)術團(tuan)隊,主(zhu)要致力于免疫學、分子生(sheng)(sheng)物(wu)學、病(bing)理、病(bing)理分子生(sheng)(sheng)物(wu)、代(dai)謝組學、生(sheng)(sheng)物(wu)化學技(ji)術在科(ke)研中(zhong)的(de)應用與推廣(guang),專業從事相關產品(pin)的(de)引(yin)進、服務(wu)和(he)研發。通過高度細分、*的(de)服務(wu)平臺(tai),向廣(guang)大客戶提供醫學科(ke)研樣本檢測、合(he)作(zuo)研究、開發及生(sheng)(sheng)產。
主推產品一:ELISA試劑盒
1、專業的ELISA試劑盒(he)生產企業
2、*抗體----高效(xiao)、靈(ling)敏、特異(yi)
3、規(gui)范(fan)包被操作----吸(xi)附均勻,吸(xi)附性好(hao),空白值低(di),孔底透(tou)明(ming)度高
4、*的優化方案----重復性高,可靠性強
5、購買本公司的ELISA試(shi)劑盒(he),免費代測
6、技術(shu)服務(wu)要(yao)求:專(zhuan)業,規(gui)范(fan),高效
7、適用(yong)于體(ti)液(ye)、組(zu)織勻(yun)漿液(ye)、細胞培養上(shang)清(qing)液(ye)、尿液(ye)等多種類(lei)型(xing)的樣本
8、可檢測(ce)動物(wu)類型(xing)豐富:人(ren)、猴、大鼠(shu)、小鼠(shu)、豚鼠(shu)、黃金地鼠(shu)、兔(tu)、豬、犬、牛、綿羊(yang)、山羊(yang)、鵝、雞、蝦、河蟹(xie)、鱸魚(yu)、斑馬魚(yu)等
9、可(ke)檢(jian)測(ce)指標齊(qi)全:炎癥因子(zi)、血管生(sheng)成素、動脈粥樣硬(ying)化因子(zi)、趨(qu)化因子(zi)、生(sheng)長(chang)因子(zi)基質金屬蛋白酶、脂肪因子(zi)等等
10、經(jing)濟、實(shi)惠(hui)、可(ke)靠(kao),完善、穩(wen)定的(de)實(shi)驗(yan)體系,優秀的(de)科(ke)研(yan)隊伍,*的(de)實(shi)驗(yan)設(she)備、準(zhun)確可(ke)靠(kao)的(de)實(shi)驗(yan)結(jie)果,是您(nin)可(ke)靠(kao)的(de)合作伙(huo)伴
我們(men)的優(you)勢:*、完善、穩定的實驗(yan)體(ti)系,優(you)秀的科(ke)研工作(zuo)者,準確(que)可(ke)靠的實驗(yan)結(jie)果(guo)。稟存用戶至上的原則,竭誠為(wei)你(ni)服(fu)務。
主推產品二:胎牛血清
慧穎生物供應:胎(tai)牛血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、新生牛血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、小(xiao)(xiao)牛血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、馬血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、山羊血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、人(ren)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、人(ren)AB血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、綿羊血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、雞血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、豬血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、封閉(bi)驢血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、大鼠血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、小(xiao)(xiao)鼠血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)。其中特殊(shu)胎(tai)牛血(xue)(xue)(xue)清(qing)(qing)(qing)(qing):超低IgG胎(tai)牛許晴(qing)、透析(xi)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、活性炭處理(li)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)以(yi)及(ji)活性炭/熱滅活血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)等,庫存常備(bei)現貨
囊(nang)括主要品牌(pai):德國(guo)(guo)SERANA、澳(ao)洲BOVOGEN、美(mei)國(guo)(guo)GIBCO、美(mei)國(guo)(guo)Hyclone以及美(mei)國(guo)(guo)SIGMA,熱賣貨(huo)號:10099-141、10270-106、SH30084.03、10437-028、SFBS、S-FBS-SA-015、16000-044
慧穎以每年5000瓶血(xue)清出庫到(dao)全國各地重(zhong)點實(shi)驗室、高校、醫(yi)院、制(zhi)藥單位等,目(mu)前成(cheng)為全國幾(ji)大(da)動物(wu)血(xue)清供應(ying)商之一。
主推產品三:細胞株細胞系
400多(duo)種類細胞(bao)株(zhu)(zhu)細胞(bao)系,20多(duo)株(zhu)(zhu)耐藥株(zhu)(zhu),公司擁有完善的細胞(bao)庫管理(li)體(ti)系,80%細胞(bao)技術人員為碩士學歷,二十多(duo)年的細胞(bao)培養經驗(yan),所售(shou)細胞(bao)背景清晰、傳(chuan)代次數少(shao)、*、售(shou)后一對一服務,跟蹤及時到(dao)位。
熱賣(mai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao):DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。