庫存現貨(huo)：人結腸癌細(xi)胞，HT-29細(xi)胞，價格/形態 全國(guo)各地(di)均可發貨，全國(guo)統一價(jia)格，運(yun)輸形式：復蘇、凍存(cun)以及(ji)全血(xue)清包被（2ML管子）。

產品名稱：HT-29細胞

中文名稱：人結腸癌細(xi)胞

來源：人結(jie)腸癌

形態(tai)特性(xing)：上皮(pi)樣

生長特性(xing)：貼壁生長

培養基：DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)  10%FBS　

培養條件：37℃，5% CO2

消(xiao)化條件：0.25% (w/v) Trypsin-0.53 mM EDTA溶液，消(xiao)化5-10min

傳化比例(li)：1：3-1：8

換液時間：2-3天換液一次

凍(dong)存液比例：85%培養基，10%FBS，5% (v/v) DMSO

凍存密(mi)度：1-3 ×10^6/管，液氮保存

活力：91％（ViabilitybyTrypanBlueExclusion）

細(xi)(xi)胞檢測：細(xi)(xi)胞不含有(you)HIV-1、HBV、HCV、支(zhi)原體(ti)、細(xi)(xi)菌、酵母(mu)和真(zhen)菌

細(xi)胞簡介：HT-29細(xi)胞是1964年由(you)Fogh J用移(yi)植培(pei)(pei)養(yang)(yang)方(fang)法和含15%FBS的(de)F12培(pei)(pei)養(yang)(yang)液(ye)從原(yuan)發性(xing)(xing)腫瘤(liu)分離(li)的(de)。近(jin)來，已建株的(de)培(pei)(pei)養(yang)(yang)細(xi)胞用含血清的(de)McCoy's 5a培(pei)(pei)養(yang)(yang)基(ji)培(pei)(pei)養(yang)(yang)。 該(gai)細(xi)胞系在裸(luo)鼠(shu)(shu)中成瘤(liu)，也能在類固醇處理(li)的(de)地鼠(shu)(shu)中成瘤(liu)。該(gai)細(xi)胞可合成IgA、CEA、TGFβ結合蛋白和黏液(ye)素(su)；表(biao)(biao)達(da)尿激酶(mei)(mei)受體，但(dan)沒(mei)有(you)檢測到血漿酶(mei)(mei)原(yuan)活(huo)性(xing)(xing)；不表(biao)(biao)達(da)CD4，但(dan)細(xi)胞表(biao)(biao)面表(biao)(biao)達(da)半乳糖神經酰胺（HIV的(de)可能替(ti)代受體）。該(gai)細(xi)胞系癌(ai)基(ji)因(yin)(yin)c-myc、K-ras、H-ras、N-ras、Myb、sis、fos陽(yang)性(xing)(xing)；p53基(ji)因(yin)(yin)過表(biao)(biao)達(da)，并且(qie)在273位(wei)密碼(ma)子處發。

慧穎生(sheng)物(wu)提供400多種細(xi)(xi)胞(bao)(bao)(bao)(bao)系細(xi)(xi)胞(bao)(bao)(bao)(bao)株,以(yi)及相(xiang)應的細(xi)(xi)胞(bao)(bao)(bao)(bao)學實驗服(fu)務:血管新生(sheng)、traswell、劃痕實驗、細(xi)(xi)胞(bao)(bao)(bao)(bao)遷移(yi)、克隆形成、細(xi)(xi)胞(bao)(bao)(bao)(bao)凋亡、細(xi)(xi)胞(bao)(bao)(bao)(bao)增(zeng)殖(zhi)、細(xi)(xi)胞(bao)(bao)(bao)(bao)周期等。另有：示蹤細(xi)(xi)胞(bao)(bao)(bao)(bao)、穩(wen)定(ding)(ding)細(xi)(xi)胞(bao)(bao)(bao)(bao)株構(gou)建、細(xi)(xi)胞(bao)(bao)(bao)(bao)代感染(ran)、篩藥(yao)細(xi)(xi)胞(bao)(bao)(bao)(bao)株產品、篩藥(yao)細(xi)(xi)胞(bao)(bao)(bao)(bao)株定(ding)(ding)制(zhi)、pathway研究細(xi)(xi)胞(bao)(bao)(bao)(bao)、STR分(fen)型(xing)鑒定(ding)(ding)，細(xi)(xi)胞(bao)(bao)(bao)(bao)染(ran)色等技術(shu)服(fu)務提供。咨(zi)詢： ！

人結(jie)腸(chang)癌細胞，HT-29細胞，價格/形態  細胞傳代培養技術：

細胞復(fu)蘇

1. 將凍存的細胞取出后迅速放入37℃水(shui)浴中，并(bing)不時搖動，在一分鐘內使其(qi)*融化

2.將(jiang)細胞吸出放(fang)入有培(pei)養基(ji)的離心管(guan)(guan)中中，用滴管(guan)(guan)輕輕吹打混勻，800轉/min，5分鐘(zhong)離心；棄去上(shang)清液，加入培(pei)養基(ji)，混勻再離心一(yi)次，棄液，加入4~5ml 培(pei)養基(ji)，混勻，移(yi)入培(pei)養瓶中

細胞換液

1.用舊液體輕輕沖洗培養(yang)瓶底(di)，然后用滴管吸盡舊液（棄去）

2.加入PBS1~2ml，輕輕搖(yao)晃(huang)，然后用(yong)滴(di)管吸盡舊液（棄去(qu)）

3.如果細胞液比較臟，可以重復(fu)步驟(zou)2

4.加入新培(pei)(pei)養(yang)基，輕輕搖(yao)勻，放(fang)入培(pei)(pei)養(yang)箱(xiang)繼續培(pei)(pei)養(yang)

細(xi)胞傳代

1.用舊(jiu)(jiu)液(ye)體輕輕沖洗培(pei)養(yang)瓶底，然后用滴管(guan)吸盡(jin)舊(jiu)(jiu)液(ye)（棄去）

2.加入PBS1~2ml，輕輕搖晃，然后用滴管吸盡舊液（棄去）

3.加入約(yue)1~1.5ml胰酶，室溫或培養(yang)箱中放置4~5分(fen)鐘，直到所有貼壁細胞消化(hua)下來為止【判斷方法：倒置相差顯微鏡觀察，細胞變圓時就可(ke)以(yi)終止消化(hua)】

4.加入(ru)約1ml培養基（含有小(xiao)牛血清）終(zhong)止消(xiao)化

5.用滴管(guan)吹洗(xi)一次，移入離心管(guan), 800轉/min，5分(fen)鐘離心

6.加入培養(yang)基(ji)，吹打分散細胞，再根據分傳細胞瓶數(shu)補加一定量(liang)的(de)含血清的(de)培養(yang)基(ji)，制成細胞懸液；分裝至2~3個培養(yang)瓶中

7.放入培(pei)養箱進行(xing)培(pei)養

細胞計(ji)數

1.準備好(hao)顯微鏡(jing)、載玻(bo)片、蓋玻(bo)片、血細(xi)胞計數板、吸管等儀器設(she)備

2.按照“細(xi)胞傳代”中講述的(de)方法(fa)制成細(xi)胞懸液

3.將細胞懸液(ye)取(qu)出(chu)若(ruo)干，滴(di)入血細胞計數板(ban)，注(zhu)意(yi)不要有氣泡(pao)進入

4.加樣后(hou)(hou)靜置(zhi)2~3min后(hou)(hou)觀察(cha)，數(shu)四角大格(ge)內的細(xi)胞總數(shu)。計(ji)數(shu)時應按照一定的路徑，以免(mian)遺漏或(huo)重(zhong)復。細(xi)胞壓線時計(ji)上不計(ji)下(xia)，計(ji)左不計(ji)右，計(ji)數(shu)2~3次，取平均值

5. 細(xi)(xi)胞密度 = 四大格中細(xi)(xi)胞總數(shu)÷4 ×104 = 細(xi)(xi)胞數(shu)/ml懸液

細(xi)胞(bao)凍存(cun)

1. 重復“細胞(bao)傳代(dai)”中(zhong)的(de)(de)消化細胞(bao)的(de)(de)步驟，將細胞(bao)消化下來，轉移(yi)至(zhi)離心管800轉/min，5分鐘離心，棄液

2. 沉淀中加入凍存液(ye)，輕吹制(zhi)成懸液(ye)

3. 分轉到凍存管內，每個1~1.5ml

4. 將凍存管口封嚴

5.貼上標簽，注明(ming)凍(dong)存(cun)時間、細胞種類及代數

6. 按下列順序降溫，并zui終凍(dong)存(cun)：室溫→ 4℃ 20min → -20℃ 30min →-70℃凍(dong)存(cun)

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慧穎生物關于出售的 污染問題，請在收到產品48小時內，給我們提出真實的實驗結果；細胞活性問題，請在收到產品7天內給我們提出真實的實驗結果，我們調查核實后予免費調換，不收取任何費用。

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慧穎以每年5000瓶血(xue)清出庫到全國各地重點(dian)實(shi)驗室、高校、醫院、制藥單位等，目前(qian)成為(wei)全國幾大(da)動(dong)物血(xue)清供應商之一。

主推產品三：細胞株細胞系

400多(duo)種類細(xi)胞(bao)株(zhu)細(xi)胞(bao)系(xi)(xi)，20多(duo)株(zhu)耐藥株(zhu)，公(gong)司擁有完善的細(xi)胞(bao)庫管理體系(xi)(xi)，80%細(xi)胞(bao)技術(shu)人員(yuan)為碩士學歷，二(er)十(shi)多(duo)年的細(xi)胞(bao)培養經驗，所(suo)售(shou)細(xi)胞(bao)背景(jing)清晰、傳代次(ci)數少(shao)、*、售(shou)后一(yi)對一(yi)服務，跟蹤(zong)及時到位。

熱賣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。

另有(you)：我(wo)司代(dai)購美國SANTA CURZ、ABCAM、CST、密理(li)博抗體，全國格(ge)，！