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人肝癌細胞,Hep G2細胞,形態/培養

  • 產品型(xing)號:
  • 產品(pin)時間:2024-08-13
  • 簡要描述(shu):人肝癌細胞,Hep G2細胞,形態/培養,慧穎提供來源,背景資料,細胞形態,培養基,培養條件,細胞詳細說明書以及注意事項等。慧穎細胞庫,擁有完善的細胞庫管理體系,供應400多種類細胞株細胞系:國內/外優質細胞供應,種類齊全,質量保證,,100%放心免費售后!自細胞庫成立至今,供應于全國各省市地區,擁有北京上海各地中科院研究所,復旦,瑞金,上藥所等各大小型院校及企業。
  • 產品簡介

庫存現貨:人(ren)肝癌細(xi)胞,Hep G2細(xi)胞,形(xing)態/培養 全國各地均可發貨,全國統一價格,運輸形式:復蘇、凍存以及全血清包被(2ML管子,適合冬季運輸,運輸途中細胞處于休眠狀態)。

產品名稱:Hep G2細胞

中文名稱:人肝(gan)癌細(xi)胞

來源:人肝癌

形態特性:上皮(pi)樣(yang)

生長(chang)特(te)性(xing):貼壁生長(chang)

培(pei)養基(ji):DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)  10%FBS 

培(pei)養條件(jian):37℃,5% CO2

消(xiao)化條件(jian):0.25% (w/v) Trypsin-0.53 mM EDTA溶液,消(xiao)化5-10min

傳化比例(li):1:3-1:8

換(huan)液時間:2-3天換(huan)液一次

凍存液比例(li):85%培(pei)養基,10%FBS,5% (v/v) DMSO

凍(dong)存密度:1-3 ×10^6/管(guan),液氮保存

活(huo)力:91%(ViabilitybyTrypanBlueExclusion)

細胞(bao)檢測:細胞(bao)不含(han)有HIV-1、HBV、HCV、支(zhi)原(yuan)體、細菌、酵(jiao)母和真菌

細胞(bao)簡介:Hep G2細胞(bao)來源于一名15歲的(de)白(bai)(bai)(bai)人(ren)少年的(de)肝癌組織。該(gai)細胞(bao)表達甲胎蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、白(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、α-2-巨球蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、α-1-抗(kang)(kang)胰蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶(mei)、轉(zhuan)鐵蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、α-1-抗(kang)(kang)凝(ning)乳蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶(mei)、結合珠蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、銅藍蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、纖溶酶(mei)原(yuan)、補體C4、C3激活物、纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)原(yuan)、α-1酸(suan)性糖蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、α-2-HS-糖蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、β-脂(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)、視黃醇結合蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai);表達胰島素受(shou)體和胰島素樣生長因子IGFⅡ的(de)受(shou)體;該(gai)細胞(bao)具有(you)3-羥基-3-甲酰輔酶(mei)A還原(yuan)酶(mei)和肝甘油三(san)酯脂(zhi)肪酶(mei)的(de)活性。目前尚未(wei)證明該(gai)細胞(bao)中有(you)HBV基因組。

慧穎生物(wu)提(ti)供400多種細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)系細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株(zhu),以及(ji)相(xiang)應的細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)學實驗服(fu)務:血管新生、traswell、劃痕實驗、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)遷移、克隆(long)形成(cheng)、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凋(diao)亡、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)增(zeng)殖、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)周(zhou)期等。另有:示蹤細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、穩定(ding)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株(zhu)構(gou)建、細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)代感染、篩(shai)藥細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株(zhu)產品、篩(shai)藥細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株(zhu)定(ding)制、pathway研究細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、STR分(fen)型(xing)鑒定(ding),細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)染色等技術服(fu)務提(ti)供。咨詢: !

人(ren)肝癌細胞(bao)(bao),Hep G2細胞(bao)(bao),形態/培養 細胞傳代培養技術:

細胞復蘇

1. 將(jiang)凍存(cun)的細胞取出后(hou)迅速放入37℃水浴(yu)中,并不時搖動,在一分鐘內使其*融化

2.將(jiang)細(xi)胞吸出放入有培(pei)養(yang)(yang)基(ji)的(de)離(li)心管中(zhong)中(zhong),用滴(di)管輕(qing)(qing)輕(qing)(qing)吹打混(hun)(hun)勻(yun),800轉/min,5分鐘離(li)心;棄(qi)去上清液,加(jia)入培(pei)養(yang)(yang)基(ji),混(hun)(hun)勻(yun)再離(li)心一(yi)次,棄(qi)液,加(jia)入4~5ml 培(pei)養(yang)(yang)基(ji),混(hun)(hun)勻(yun),移入培(pei)養(yang)(yang)瓶中(zhong)

細(xi)胞換液

1.用舊(jiu)液(ye)體輕(qing)輕(qing)沖洗培養瓶底,然后用滴(di)管吸(xi)盡舊(jiu)液(ye)(棄去)

2.加入PBS1~2ml,輕(qing)輕(qing)搖晃,然后用滴管吸盡(jin)舊(jiu)液(棄去)

3.如(ru)果細胞液比(bi)較臟,可以重復步驟2

4.加入新培養(yang)(yang)基,輕輕搖勻,放入培養(yang)(yang)箱(xiang)繼(ji)續培養(yang)(yang)

細胞傳(chuan)代

1.用(yong)(yong)舊液(ye)體輕輕沖(chong)洗培(pei)養(yang)瓶(ping)底,然后用(yong)(yong)滴管吸(xi)盡舊液(ye)(棄去)

2.加入PBS1~2ml,輕(qing)輕(qing)搖晃(huang),然后用滴管吸(xi)盡(jin)舊(jiu)液(棄去)

3.加入約1~1.5ml胰酶(mei),室溫或培養(yang)箱中放置4~5分鐘,直(zhi)到所(suo)有(you)貼壁細胞消(xiao)化(hua)下來(lai)為止(zhi)【判斷方(fang)法:倒置相差顯(xian)微(wei)鏡觀察,細胞變圓時就(jiu)可以終止(zhi)消(xiao)化(hua)】

4.加入約1ml培養基(含有小牛血清(qing))終止(zhi)消(xiao)化

5.用滴管吹洗一次,移入離心管, 800轉/min,5分鐘離心

6.加入培(pei)養(yang)基(ji),吹打分(fen)(fen)散細(xi)胞,再根據分(fen)(fen)傳細(xi)胞瓶(ping)數(shu)補加一定量(liang)的(de)(de)含血(xue)清的(de)(de)培(pei)養(yang)基(ji),制成細(xi)胞懸(xuan)液(ye);分(fen)(fen)裝至(zhi)2~3個培(pei)養(yang)瓶(ping)中(zhong)

7.放入培養(yang)(yang)箱進行培養(yang)(yang)

細胞(bao)計數(shu)

1.準備(bei)好(hao)顯微鏡、載玻片、蓋玻片、血細胞計數板(ban)、吸管(guan)等儀器(qi)設備(bei)

2.按照“細(xi)(xi)胞(bao)傳代”中講述(shu)的方法制成細(xi)(xi)胞(bao)懸液

3.將(jiang)細胞(bao)懸(xuan)液取出若干,滴入(ru)血細胞(bao)計(ji)數板,注意不要(yao)有氣泡進入(ru)

4.加(jia)樣后靜置2~3min后觀察,數(shu)(shu)四角大格(ge)內(nei)的細胞總(zong)數(shu)(shu)。計(ji)數(shu)(shu)時應(ying)按照一定(ding)的路徑,以免遺(yi)漏或(huo)重(zhong)復。細胞壓線時計(ji)上不(bu)計(ji)下,計(ji)左不(bu)計(ji)右,計(ji)數(shu)(shu)2~3次,取平均值

5. 細(xi)胞(bao)密度 = 四(si)大格中(zhong)細(xi)胞(bao)總數÷4 ×104 = 細(xi)胞(bao)數/ml懸液

細(xi)胞凍存

1. 重復“細(xi)(xi)胞傳代(dai)”中的(de)消化(hua)細(xi)(xi)胞的(de)步驟,將細(xi)(xi)胞消化(hua)下來(lai),轉(zhuan)移至(zhi)離心管800轉(zhuan)/min,5分鐘離心,棄液(ye)

2. 沉淀中加入凍存(cun)液(ye),輕吹制成懸液(ye)

3. 分轉到凍存管內,每(mei)個(ge)1~1.5ml

4. 將凍存(cun)管口封嚴(yan)

5.貼上標簽,注(zhu)明(ming)凍存時間(jian)、細(xi)胞種類及(ji)代數(shu)

6. 按下列順序降溫,并zui終凍存:室溫→ 4℃ 20min → -20℃ 30min →-70℃凍存

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9、可檢(jian)測指標齊(qi)全(quan):炎癥(zheng)因子(zi)、血管(guan)生(sheng)成素、動脈(mo)粥樣硬化(hua)因子(zi)、趨化(hua)因子(zi)、生(sheng)長(chang)因子(zi)基質金屬蛋(dan)白(bai)酶、脂肪因子(zi)等等

10、經濟、實(shi)惠、可(ke)靠,完善、穩定的(de)(de)實(shi)驗(yan)(yan)體系,優秀的(de)(de)科研(yan)隊伍,*的(de)(de)實(shi)驗(yan)(yan)設(she)備、準確可(ke)靠的(de)(de)實(shi)驗(yan)(yan)結果,是(shi)您可(ke)靠的(de)(de)合作伙伴

 我們的(de)(de)(de)優勢:*、完善、穩定的(de)(de)(de)實(shi)(shi)驗體系,優秀的(de)(de)(de)科研工(gong)作者,準確可靠的(de)(de)(de)實(shi)(shi)驗結(jie)果。稟存用戶至上的(de)(de)(de)原則,竭誠(cheng)為(wei)你服(fu)務(wu)。

主推產品二:胎牛血清

慧穎生(sheng)(sheng)物供應(ying):胎牛(niu)(niu)血(xue)清(qing)(qing)(qing)、新(xin)生(sheng)(sheng)牛(niu)(niu)血(xue)清(qing)(qing)(qing)、小牛(niu)(niu)血(xue)清(qing)(qing)(qing)、馬血(xue)清(qing)(qing)(qing)、山羊血(xue)清(qing)(qing)(qing)、人(ren)血(xue)清(qing)(qing)(qing)、人(ren)AB血(xue)清(qing)(qing)(qing)、綿羊血(xue)清(qing)(qing)(qing)、雞(ji)血(xue)清(qing)(qing)(qing)、豬血(xue)清(qing)(qing)(qing)、封閉驢血(xue)清(qing)(qing)(qing)、大鼠(shu)血(xue)清(qing)(qing)(qing)、小鼠(shu)血(xue)清(qing)(qing)(qing)。其中特(te)殊胎牛(niu)(niu)血(xue)清(qing)(qing)(qing):超低IgG胎牛(niu)(niu)許晴、透(tou)析血(xue)清(qing)(qing)(qing)、活性炭(tan)處理血(xue)清(qing)(qing)(qing)以及活性炭(tan)/熱滅活血(xue)清(qing)(qing)(qing)等,庫存常備現(xian)貨

囊(nang)括(kuo)主要(yao)品(pin)牌:德(de)國SERANA、澳(ao)洲BOVOGEN、美(mei)國GIBCO、美(mei)國Hyclone以(yi)及(ji)美(mei)國SIGMA,熱賣貨號:10099-141、10270-106、SH30084.03、10437-028、SFBS、S-FBS-SA-015、16000-044

慧(hui)穎(ying)以每年(nian)5000瓶血(xue)清(qing)出庫(ku)到全國(guo)各(ge)地重點實驗室、高(gao)校、醫院、制藥單位等,目(mu)前成(cheng)為全國(guo)幾大動物血(xue)清(qing)供(gong)應商之(zhi)一。

主推產品三:細胞株細胞系

400多種類細胞株細胞系(xi),20多株耐藥株,公司擁有完善的細胞庫管(guan)理體(ti)系(xi),80%細胞技術人員(yuan)為碩(shuo)士學(xue)歷,二十(shi)多年(nian)的細胞培養經驗(yan),所售細胞背景(jing)清晰、傳代次數(shu)少、*、售后一對一服務,跟蹤及時到位。

熱賣細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao):DC2.4細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。

另有:我司(si)代購(gou)美(mei)國(guo)SANTA CURZ、ABCAM、CST、密理博抗(kang)體,全國(guo)格,!

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