聯系電話:
庫存現貨:大鼠(shu)腦膠(jiao)質瘤細胞,C6細胞,價格(ge)/形(xing)態全國各地均可發貨,全國統一價格,運輸形式:復蘇、凍存以及全血清包被(2ML管子,適合冬季運輸,運輸途中細胞處于休眠狀態)。
產品名稱(cheng):C6細胞(bao)
中文(wen)名稱:大(da)鼠腦膠(jiao)質瘤細胞
來(lai)源:大(da)鼠腦膠質(zhi)瘤
形態(tai)特(te)性:上皮樣(yang)
生(sheng)長(chang)特(te)性:貼壁生(sheng)長(chang)
培養(yang)基:F12K 馬血(xue)清,15%;優質胎牛血(xue)清,2.5%
培養條(tiao)件:37℃,5% CO2
消化條件:0.25% (w/v) Trypsin-0.53 mM EDTA溶液(ye),消化3-5分鐘min
傳化(hua)比例:1:3-1:8
換(huan)液(ye)時間:2-3天換(huan)液(ye)一次
凍存液(ye)比例(li):85%培養基,10%FBS,5% (v/v) DMSO
凍存密度:1-3 ×10^6/管,液氮保存
活力:91%(ViabilitybyTrypanBlueExclusion)
細胞檢測:細胞不含有HIV-1、HBV、HCV、支原體(ti)、細菌(jun)、酵母和真菌(jun)
細(xi)胞簡介:膠質(zhi)細胞株(zhu)C6是由Benda等用N-nitrosomethylurea誘導的(de)(de)大鼠膠質瘤克(ke)隆,并經(jing)過(guo)一(yi)系列的(de)(de)體外培養(yang)和動物傳(chuan)代交替后建成的(de)(de)。 當細胞從低密度(du)生長到(dao)滿瓶時,S-100產量增加(jia)10倍。
慧穎生物(wu)提(ti)供400多種細胞(bao)(bao)系細胞(bao)(bao)株(zhu),以(yi)及相應的細胞(bao)(bao)學實(shi)驗服務:血(xue)管新生、traswell、劃(hua)痕實(shi)驗、細胞(bao)(bao)遷移、克隆形成、細胞(bao)(bao)凋亡、細胞(bao)(bao)增(zeng)殖、細胞(bao)(bao)周(zhou)期等。另有:示蹤細胞(bao)(bao)、穩定(ding)(ding)(ding)細胞(bao)(bao)株(zhu)構建(jian)、細胞(bao)(bao)代感染、篩藥細胞(bao)(bao)株(zhu)產品(pin)、篩藥細胞(bao)(bao)株(zhu)定(ding)(ding)(ding)制、pathway研究細胞(bao)(bao)、STR分(fen)型鑒定(ding)(ding)(ding),細胞(bao)(bao)染色等技術服務提(ti)供。咨(zi)詢(xun): !
大鼠腦膠質瘤細胞(bao),C6細胞(bao),價(jia)格/形態 細胞傳(chuan)代培養技術:
細胞復蘇
1. 將凍(dong)存(cun)的細胞取出后迅速放(fang)入37℃水浴中,并(bing)不(bu)時搖動,在一分鐘內使(shi)其(qi)*融化
2.將細(xi)胞吸(xi)出放入有培(pei)養基的離(li)心管(guan)中中,用滴管(guan)輕輕吹打混勻(yun),800轉/min,5分鐘離(li)心;棄去(qu)上清液(ye),加(jia)入培(pei)養基,混勻(yun)再離(li)心一(yi)次,棄液(ye),加(jia)入4~5ml 培(pei)養基,混勻(yun),移(yi)入培(pei)養瓶中
細胞(bao)換液
1.用舊(jiu)液(ye)體輕(qing)輕(qing)沖洗培養瓶底,然后用滴管(guan)吸盡(jin)舊(jiu)液(ye)(棄去)
2.加入(ru)PBS1~2ml,輕輕搖晃,然后用滴(di)管吸盡舊液(棄去)
3.如果細(xi)胞液(ye)比較臟,可以重復步驟2
4.加入新培養(yang)(yang)基,輕(qing)輕(qing)搖勻,放入培養(yang)(yang)箱(xiang)繼(ji)續培養(yang)(yang)
細胞傳代(dai)
1.用(yong)舊液(ye)體(ti)輕輕沖洗培養瓶(ping)底,然后用(yong)滴(di)管吸盡舊液(ye)(棄去)
2.加入PBS1~2ml,輕(qing)輕(qing)搖晃,然后(hou)用滴管吸(xi)盡(jin)舊液(棄去)
3.加入約1~1.5ml胰酶,室(shi)溫或(huo)培(pei)養箱中放置(zhi)4~5分鐘(zhong),直(zhi)到(dao)所有貼壁細胞消化下來為止(zhi)【判(pan)斷方法:倒(dao)置(zhi)相差顯微鏡觀(guan)察,細胞變圓(yuan)時就可以終止(zhi)消化】
4.加入約(yue)1ml培(pei)養基(ji)(含(han)有小牛血清)終止消化
5.用(yong)滴管吹洗一次,移入離(li)心管, 800轉(zhuan)/min,5分鐘離(li)心
6.加入培養基(ji),吹打(da)分散細(xi)胞,再根(gen)據分傳細(xi)胞瓶數補加一定量的(de)含血清的(de)培養基(ji),制成細(xi)胞懸液;分裝至(zhi)2~3個培養瓶中
7.放入培養箱進行培養
細胞計數
1.準備好(hao)顯(xian)微(wei)鏡(jing)、載玻(bo)片(pian)、蓋(gai)玻(bo)片(pian)、血(xue)細(xi)胞計數板、吸(xi)管等儀(yi)器設備
2.按照“細胞傳(chuan)代(dai)”中講述的方(fang)法制成細胞懸(xuan)液(ye)
3.將細(xi)胞(bao)懸(xuan)液(ye)取出若(ruo)干,滴入血(xue)細(xi)胞(bao)計(ji)數板,注(zhu)意不(bu)要有氣泡進入
4.加樣(yang)后靜置(zhi)2~3min后觀察(cha),數(shu)四角(jiao)大(da)格內的細(xi)胞(bao)(bao)總數(shu)。計數(shu)時應按照一定的路徑,以免遺漏(lou)或重復。細(xi)胞(bao)(bao)壓線時計上不(bu)計下,計左不(bu)計右,計數(shu)2~3次,取平(ping)均值
5. 細胞密(mi)度 = 四(si)大格中細胞總數÷4 ×104 = 細胞數/ml懸液
細胞(bao)凍存
1. 重復(fu)“細胞傳代”中(zhong)的(de)消化細胞的(de)步(bu)驟,將細胞消化下來,轉移(yi)至離(li)心(xin)管800轉/min,5分鐘離(li)心(xin),棄(qi)液
2. 沉淀中加入(ru)凍存液,輕吹制成懸液
3. 分轉(zhuan)到凍存管內,每個1~1.5ml
4. 將凍存管口(kou)封嚴
5.貼(tie)上標(biao)簽,注明凍存(cun)時間、細胞種類及代數
6. 按下列順(shun)序降溫(wen),并zui終凍(dong)存:室溫(wen)→ 4℃ 20min → -20℃ 30min →-70℃凍(dong)存
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慧穎生物關于出售的 污染問題,請在收到產品48小時內,給我們提出真實的實驗結果;細胞活性問題,請在收到產品7天內給我們提出真實的實驗結果,我們調查核實后予免費調換,不收取任何費用。
需要客(ke)戶提供細(xi)胞培(pei)養說明、細(xi)胞操作處理方法、細(xi)胞質量問(wen)題反饋(kui)表(biao);如果搞不清原因的,或(huo)者客(ke)戶直(zhi)接(jie)愿意(yi)支付(fu)購買價(jia)半價(jia)的費用直(zhi)接(jie)復蘇。
如用戶收到(dao)細(xi)胞(bao)(bao)8-30天(tian)之(zhi)內,因處理(li)不當造成細(xi)胞(bao)(bao)死亡(wang)或污染,我公(gong)司(si)將以五折(zhe)優(you)惠價(jia)重新(xin)提供一(yi)株原(yuan)細(xi)胞(bao)(bao)。
慧穎生物(wu)(wu)科(ke)技(上海(hai))有(you)限公司位(wei)于嘉(jia)定區南翔眾仁路399號運通星財富廣(guang)場,由海(hai)外華僑和多名中科(ke)院博(bo)士組成的技術團隊,主要致(zhi)力(li)于免疫學(xue)、分子(zi)生物(wu)(wu)學(xue)、病理、病理分子(zi)生物(wu)(wu)、代謝組學(xue)、生物(wu)(wu)化學(xue)技術在科(ke)研中的應(ying)用與推廣(guang),專(zhuan)業從(cong)事(shi)相關產(chan)品的引(yin)進(jin)、服務(wu)和研發(fa)。通過(guo)高度(du)細分、*的服務(wu)平臺,向廣(guang)大(da)客(ke)戶提供醫學(xue)科(ke)研樣本檢測、合作研究、開(kai)發(fa)及生產(chan)。
主推產品一:ELISA試劑(ji)盒
1、專業的ELISA試劑盒(he)生產(chan)企業
2、*抗(kang)體----高效、靈敏、特異
3、規范包被操(cao)作(zuo)----吸(xi)附(fu)均勻,吸(xi)附(fu)性(xing)好,空白(bai)值低,孔底透(tou)明度高
4、*的優化方案----重復性(xing)高,可靠性(xing)強
5、購(gou)買本(ben)公司的ELISA試劑(ji)盒,免費代(dai)測
6、技術(shu)服(fu)務要求:專業,規范,高效
7、適用(yong)于體液、組(zu)織勻漿(jiang)液、細胞(bao)培(pei)養上清液、尿(niao)液等多種類型的樣本
8、可檢(jian)測動物類型豐富:人、猴(hou)、大鼠、小鼠、豚鼠、黃金地鼠、兔、豬、犬(quan)、牛、綿羊、山羊、鵝、雞、蝦、河蟹、鱸魚、斑馬魚等
9、可檢測指標(biao)齊全:炎癥因子、血管(guan)生(sheng)成素、動(dong)脈粥樣硬化(hua)(hua)因子、趨(qu)化(hua)(hua)因子、生(sheng)長(chang)因子基(ji)質金屬(shu)蛋白酶(mei)、脂肪因子等等
10、經濟(ji)、實(shi)惠、可靠(kao),完善、穩定的實(shi)驗(yan)體系,優(you)秀的科研隊(dui)伍,*的實(shi)驗(yan)設備、準確可靠(kao)的實(shi)驗(yan)結果,是您可靠(kao)的合作(zuo)伙(huo)伴
我(wo)們的(de)(de)(de)優勢:*、完善、穩(wen)定的(de)(de)(de)實驗體(ti)系,優秀的(de)(de)(de)科研工作者,準確可靠的(de)(de)(de)實驗結(jie)果。稟(bing)存用戶至上的(de)(de)(de)原則(ze),竭誠為你(ni)服務(wu)。
主推產(chan)品二:胎牛血清
慧(hui)穎生物供應:胎(tai)牛(niu)(niu)血(xue)(xue)清(qing)(qing)、新生牛(niu)(niu)血(xue)(xue)清(qing)(qing)、小(xiao)牛(niu)(niu)血(xue)(xue)清(qing)(qing)、馬血(xue)(xue)清(qing)(qing)、山羊血(xue)(xue)清(qing)(qing)、人血(xue)(xue)清(qing)(qing)、人AB血(xue)(xue)清(qing)(qing)、綿羊血(xue)(xue)清(qing)(qing)、雞血(xue)(xue)清(qing)(qing)、豬血(xue)(xue)清(qing)(qing)、封(feng)閉驢(lv)血(xue)(xue)清(qing)(qing)、大鼠(shu)血(xue)(xue)清(qing)(qing)、小(xiao)鼠(shu)血(xue)(xue)清(qing)(qing)。其中特殊(shu)胎(tai)牛(niu)(niu)血(xue)(xue)清(qing)(qing):超低IgG胎(tai)牛(niu)(niu)許晴、透析血(xue)(xue)清(qing)(qing)、活(huo)(huo)性炭(tan)處理血(xue)(xue)清(qing)(qing)以及(ji)活(huo)(huo)性炭(tan)/熱滅活(huo)(huo)血(xue)(xue)清(qing)(qing)等,庫存常備(bei)現(xian)貨
囊括(kuo)主要品牌:德國SERANA、澳洲(zhou)BOVOGEN、美(mei)(mei)國GIBCO、美(mei)(mei)國Hyclone以及美(mei)(mei)國SIGMA,熱賣貨號(hao):10099-141、10270-106、SH30084.03、10437-028、SFBS、S-FBS-SA-015、16000-044
慧穎以每年5000瓶(ping)血(xue)清出庫到(dao)全國(guo)各地重(zhong)點實驗室、高校、醫院(yuan)、制藥單位等(deng),目(mu)前成為全國(guo)幾大動物血(xue)清供應商之一。
主(zhu)推產品(pin)三:細胞(bao)株細胞(bao)系
400多種類細(xi)(xi)(xi)胞(bao)(bao)株(zhu)細(xi)(xi)(xi)胞(bao)(bao)系(xi),20多株(zhu)耐藥株(zhu),公司擁有完善的(de)細(xi)(xi)(xi)胞(bao)(bao)庫管理體系(xi),80%細(xi)(xi)(xi)胞(bao)(bao)技術人員為(wei)碩(shuo)士(shi)學歷,二十多年的(de)細(xi)(xi)(xi)胞(bao)(bao)培養經驗(yan),所售(shou)細(xi)(xi)(xi)胞(bao)(bao)背景清晰、傳代次數少、*、售(shou)后一對一服務,跟蹤及時到(dao)位。
熱賣(mai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao):DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)等。
另有:我司代購美(mei)國SANTA CURZ、ABCAM、CST、密理博抗體(ti)(ti),全國格,!