庫存現貨：人(ren)腦膠質母細胞(bao)瘤細胞(bao)，A172細胞(bao)，價格/說明書全國各地均可發貨，全國統一價格，運輸形式：復蘇、凍存以及全血清包被（2ML管子，適合冬季運輸，運輸途中細胞處于休眠狀態）。

產品(pin)名稱：A172細(xi)胞

中文名稱：人腦膠質母細胞瘤細胞

來源：人腦膠質母細胞瘤

形態特性：上皮(pi)樣

生(sheng)長特性：貼壁生(sheng)長

培養基：DMEM 90%, Fetal Bovine Serum 10%

培(pei)養條件：37℃ 5% CO2

消(xiao)化條(tiao)件：0.25% (w/v) Trypsin-0.53 mM EDTA溶(rong)液(ye)，消(xiao)化3-5min

傳化比例：1：3-1：8

換液(ye)時間：2-3天換液(ye)一次(ci)

凍存液比例：85%培養基，10%FBS, 5% (v/v) DMSO

凍存(cun)密度(du)：1-3 ×10^6/管，液氮保存(cun)

活(huo)力：94％（ViabilitybyTrypanBlueExclusion）

細胞檢測：細胞不含有HIV-1、HBV、HCV、支(zhi)原體、細菌、酵母和真菌

細(xi)胞簡介：在半(ban)固體培養基中形成克隆。 在免疫抑制小鼠中不成瘤。

慧(hui)穎生物提供(gong)(gong)400多種細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)系(xi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)(zhu),以及(ji)相(xiang)應的細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)學實驗(yan)服(fu)務(wu):血管(guan)新生、traswell、劃痕實驗(yan)、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)遷移(yi)、克隆形成(cheng)、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凋亡(wang)、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增殖(zhi)、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周期等。另有：示蹤細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、穩定(ding)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)(zhu)構建、細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)代(dai)感染、篩藥(yao)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)(zhu)產(chan)品、篩藥(yao)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)(zhu)定(ding)制、pathway研究細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、STR分型鑒定(ding)，細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)染色等技術服(fu)務(wu)提供(gong)(gong)。咨詢： ！

人腦膠質母細(xi)胞瘤細(xi)胞，A172細(xi)胞，價格/說明(ming)書(shu)  細胞傳代培養技術：

細胞復蘇

1. 將(jiang)凍存(cun)的細胞取出后迅速放入37℃水浴中，并不時(shi)搖動，在一分鐘(zhong)內使其*融化

2.將(jiang)細胞吸出放入(ru)(ru)有培養(yang)(yang)基的離(li)心(xin)(xin)管中中，用滴管輕(qing)輕(qing)吹打混(hun)勻，800轉/min，5分(fen)鐘離(li)心(xin)(xin)；棄去(qu)上清液，加入(ru)(ru)培養(yang)(yang)基，混(hun)勻再離(li)心(xin)(xin)一次，棄液，加入(ru)(ru)4~5ml 培養(yang)(yang)基，混(hun)勻，移入(ru)(ru)培養(yang)(yang)瓶中

細胞換液

1.用舊(jiu)液(ye)體輕輕沖洗培養瓶底，然后用滴管(guan)吸(xi)盡舊(jiu)液(ye)（棄去）

2.加入PBS1~2ml，輕(qing)輕(qing)搖晃，然后用滴(di)管吸盡舊(jiu)液（棄去(qu)）

3.如果細胞(bao)液比較臟，可以重復步驟2

4.加入新培(pei)養(yang)(yang)基，輕(qing)輕(qing)搖勻，放入培(pei)養(yang)(yang)箱繼續(xu)培(pei)養(yang)(yang)

細胞傳代

1.用舊液(ye)(ye)體輕輕沖洗培養(yang)瓶底，然后用滴管吸盡舊液(ye)(ye)（棄去）

2.加(jia)入PBS1~2ml，輕輕搖晃，然后用(yong)滴(di)管吸盡舊液（棄去(qu)）

3.加(jia)入約1~1.5ml胰(yi)酶，室溫或培養箱中放(fang)置4~5分(fen)鐘，直到(dao)所有貼壁細(xi)胞消(xiao)化下(xia)來為(wei)止【判斷方法：倒置相(xiang)差顯微鏡觀(guan)察，細(xi)胞變圓(yuan)時(shi)就(jiu)可(ke)以(yi)終止消(xiao)化】

4.加入(ru)約1ml培養基（含有小牛血(xue)清(qing)）終止消化

5.用(yong)滴管吹洗一(yi)次，移(yi)入離心管, 800轉/min，5分(fen)鐘(zhong)離心

6.加入培養基(ji)，吹打(da)分(fen)散細(xi)(xi)胞，再根(gen)據分(fen)傳細(xi)(xi)胞瓶數補加一定量的(de)含血(xue)清的(de)培養基(ji)，制成細(xi)(xi)胞懸液(ye)；分(fen)裝至2~3個培養瓶中(zhong)

7.放入培(pei)養箱(xiang)進行培(pei)養

細胞計數

1.準備好顯(xian)微鏡、載玻片、蓋玻片、血細胞計(ji)數板(ban)、吸(xi)管等儀器(qi)設備

2.按(an)照“細胞傳代”中講述的(de)方法制成細胞懸液

3.將細(xi)胞懸液取出若干，滴(di)入血細(xi)胞計(ji)數板，注意不要(yao)有氣(qi)泡進入

4.加樣后靜置2~3min后觀察，數四(si)角大格內(nei)的細(xi)(xi)胞總(zong)數。計(ji)(ji)數時應按照一定的路徑，以免(mian)遺漏或重復。細(xi)(xi)胞壓線(xian)時計(ji)(ji)上不計(ji)(ji)下，計(ji)(ji)左不計(ji)(ji)右，計(ji)(ji)數2~3次，取平(ping)均值

5. 細(xi)胞密度 = 四大格中細(xi)胞總數(shu)÷4 ×104 = 細(xi)胞數(shu)/ml懸液

細胞凍存

1. 重復(fu)“細胞(bao)傳代”中的消化細胞(bao)的步驟，將細胞(bao)消化下來，轉移至離心管800轉/min，5分鐘離心，棄液(ye)

2. 沉淀中(zhong)加(jia)入(ru)凍存液(ye)(ye)，輕(qing)吹制(zhi)成懸液(ye)(ye)

3. 分轉(zhuan)到凍(dong)存(cun)管內，每個1~1.5ml

4. 將凍(dong)存管口封嚴(yan)

5.貼(tie)上標簽，注明凍存時間、細(xi)胞種類及代數

6. 按下列順(shun)序降(jiang)溫，并zui終凍存(cun)：室溫→ 4℃ 20min → -20℃ 30min →-70℃凍存(cun)

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慧穎生物關于出售的污染問題，請在收到產品48小時內，給我們提出真實的實驗結果；細胞活性問題，請在收到產品7天內給我們提出真實的實驗結果，我們調查核實后予免費調換，不收取任何費用。

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 我們的優(you)勢：*、完善(shan)、穩定的實驗體系，優(you)秀的科研工作(zuo)者，準(zhun)確可靠的實驗結果。稟存用戶至(zhi)上的原則，竭誠(cheng)為(wei)你(ni)服務(wu)。

主推產品二：胎牛血清

慧穎生物供應：胎(tai)牛(niu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、新生牛(niu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、小牛(niu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、馬血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、山羊血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、人血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、人AB血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、綿羊血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、雞血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、豬血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、封閉驢血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、大鼠(shu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、小鼠(shu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)。其(qi)中特殊(shu)胎(tai)牛(niu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)：超低(di)IgG胎(tai)牛(niu)許晴、透(tou)析血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)、活(huo)性炭處(chu)理(li)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)以及活(huo)性炭/熱滅(mie)活(huo)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)等，庫(ku)存常備現貨(huo)

囊括主要(yao)品牌：德國(guo)SERANA、澳洲(zhou)BOVOGEN、美(mei)國(guo)GIBCO、美(mei)國(guo)Hyclone以及美(mei)國(guo)SIGMA，熱賣貨號：10099-141、10270-106、SH30084.03、10437-028、SFBS、S-FBS-SA-015、16000-044等。

慧穎生物(wu)攜(xie)手(shou)兄弟公司素(su)爾生物(wu)為(wei)澳洲BOVOGEN和德(de)國SERANA中國區一級(ji)代理權

慧(hui)穎以每年5000瓶血(xue)清出庫到全國各地重點實驗(yan)室、高校、醫院、制藥單位等，目前成為全國幾大動物血(xue)清供應(ying)商之一。

主推產品三：細胞株細胞系

400多種類細(xi)胞(bao)(bao)株(zhu)細(xi)胞(bao)(bao)系，20多株(zhu)耐藥株(zhu)，公司擁有完善的細(xi)胞(bao)(bao)庫(ku)管理體(ti)系，80%細(xi)胞(bao)(bao)技術人員為碩(shuo)士學(xue)歷，二十多年的細(xi)胞(bao)(bao)培養經驗，所售細(xi)胞(bao)(bao)背景清晰、傳代(dai)次數少、*、售后一對一服務(wu)，跟(gen)蹤(zong)及時(shi)到位。

熱賣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)：DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)等(deng)。

另有：我司代購美(mei)國SANTA CURZ、ABCAM、CST、密理博(bo)抗體(ti)，全(quan)國格(ge)，！