庫存現貨：人胃癌細胞，Hs 746T細胞，價格/說明書全國各地均可發貨，全國統一價格，運輸形式：復蘇、凍存以及全血清包被（2ML管子）。

產品名稱：Hs 746T細胞(bao)；Hs -746T細胞(bao)

中文名稱(cheng)：人胃癌細胞

來源：人(ren)胃癌

形態特性：上皮樣

生長特性：貼壁生長

培養基(ji)：DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)  10%FBS　

培養條(tiao)件：37℃，5% CO2

消化條(tiao)件：0.25% (w/v) Trypsin-0.53 mM EDTA溶液，消化5-10min

傳化比例：1：3-1：8

換(huan)液時(shi)間(jian)：2-3天(tian)換(huan)液一次

凍存液比例：85%培(pei)養基，10%FBS，5% (v/v) DMSO

凍存(cun)密度：1-3 ×10^6/管，液氮保(bao)存(cun)

活力(li)：91％（ViabilitybyTrypanBlueExclusion）

細胞檢測(ce)：細胞不含(han)有HIV-1、HBV、HCV、支原(yuan)體、細菌、酵母和真菌

細(xi)(xi)(xi)(xi)胞(bao)簡介：Hs 746T細(xi)(xi)(xi)(xi)胞(bao)1973年建(jian)系（Naval biosciences laboratory）。該細(xi)(xi)(xi)(xi)胞(bao)系的(de)(de)細(xi)(xi)(xi)(xi)胞(bao)大小(xiao)，形狀及核狀態均會在培養(yang)過程中發生變化(hua)。由于細(xi)(xi)(xi)(xi)胞(bao)的(de)(de)惡性化(hua)，染色多為亞三倍體（46%）。  

慧(hui)穎生(sheng)物提(ti)供400多種細(xi)(xi)胞(bao)(bao)(bao)系細(xi)(xi)胞(bao)(bao)(bao)株,以及相應(ying)的細(xi)(xi)胞(bao)(bao)(bao)學實驗服務(wu):血管新生(sheng)、traswell、劃痕實驗、細(xi)(xi)胞(bao)(bao)(bao)遷移、克隆形成、細(xi)(xi)胞(bao)(bao)(bao)凋(diao)亡、細(xi)(xi)胞(bao)(bao)(bao)增殖、細(xi)(xi)胞(bao)(bao)(bao)周期等(deng)。另有：示蹤細(xi)(xi)胞(bao)(bao)(bao)、穩定(ding)細(xi)(xi)胞(bao)(bao)(bao)株構建、細(xi)(xi)胞(bao)(bao)(bao)代感(gan)染、篩藥(yao)(yao)細(xi)(xi)胞(bao)(bao)(bao)株產品、篩藥(yao)(yao)細(xi)(xi)胞(bao)(bao)(bao)株定(ding)制、pathway研究細(xi)(xi)胞(bao)(bao)(bao)、STR分型鑒定(ding)，細(xi)(xi)胞(bao)(bao)(bao)染色等(deng)技術服務(wu)提(ti)供。咨(zi)詢： ！

人(ren)胃(wei)癌(ai)細胞(bao)，Hs 746T細胞(bao)，價格/說明書  細胞傳代培養技術：

細胞復蘇

1. 將凍存的細胞(bao)取出后迅(xun)速(su)放(fang)入(ru)37℃水浴中，并(bing)不(bu)時搖(yao)動，在一分鐘內使其*融化

2.將細胞吸出放入(ru)有培養基的離心(xin)管中中，用滴管輕(qing)(qing)輕(qing)(qing)吹打混(hun)勻，800轉(zhuan)/min，5分鐘(zhong)離心(xin)；棄去(qu)上清(qing)液(ye)，加(jia)入(ru)培養基，混(hun)勻再離心(xin)一次，棄液(ye)，加(jia)入(ru)4~5ml 培養基，混(hun)勻，移(yi)入(ru)培養瓶中

細(xi)胞換(huan)液

1.用舊(jiu)(jiu)液體輕(qing)輕(qing)沖洗培養瓶(ping)底，然后(hou)用滴管吸盡舊(jiu)(jiu)液（棄去）

2.加(jia)入PBS1~2ml，輕輕搖晃，然后用滴管吸盡舊(jiu)液（棄(qi)去）

3.如(ru)果(guo)細(xi)胞(bao)液比較臟(zang)，可以重復步驟2

4.加(jia)入新(xin)培養(yang)基，輕(qing)輕(qing)搖勻，放入培養(yang)箱繼續培養(yang)

細胞傳代

1.用舊(jiu)液體輕輕沖洗(xi)培(pei)養瓶底，然后用滴管吸盡舊(jiu)液（棄去(qu)）

2.加入PBS1~2ml，輕輕搖(yao)晃，然后用滴管吸盡舊液（棄(qi)去）

3.加入(ru)約1~1.5ml胰(yi)酶(mei)，室溫或培養箱中(zhong)放置4~5分鐘(zhong)，直到所(suo)有貼壁細胞(bao)消化(hua)下來為止(zhi)(zhi)【判斷方法(fa)：倒置相差顯微鏡觀察，細胞(bao)變圓時(shi)就可以終止(zhi)(zhi)消化(hua)】

4.加入約1ml培(pei)養基(ji)（含(han)有小牛血清(qing)）終(zhong)止消(xiao)化

5.用滴(di)管吹(chui)洗一次，移入離心(xin)管, 800轉/min，5分鐘離心(xin)

6.加入培養(yang)基，吹打分散細胞，再根據分傳(chuan)細胞瓶數補加一定量的(de)含血(xue)清的(de)培養(yang)基，制成(cheng)細胞懸液；分裝至2~3個培養(yang)瓶中

7.放入培(pei)養(yang)箱進行培(pei)養(yang)

細胞計數(shu)

1.準備好顯微鏡、載玻片(pian)、蓋(gai)玻片(pian)、血細(xi)胞計(ji)數(shu)板、吸管等(deng)儀(yi)器(qi)設備

2.按(an)照(zhao)“細胞(bao)傳代”中講述的方法制成細胞(bao)懸(xuan)液

3.將(jiang)細(xi)胞(bao)懸液取出(chu)若干，滴入血細(xi)胞(bao)計數板，注意不要有(you)氣(qi)泡進入

4.加樣后靜置2~3min后觀察(cha)，數(shu)四角大格內的細(xi)胞總(zong)數(shu)。計(ji)數(shu)時應按(an)照一定的路徑，以免遺漏(lou)或重復(fu)。細(xi)胞壓線時計(ji)上(shang)不(bu)計(ji)下(xia)，計(ji)左不(bu)計(ji)右，計(ji)數(shu)2~3次(ci)，取平均值(zhi)

5. 細(xi)胞密度(du) = 四大格(ge)中(zhong)細(xi)胞總數÷4 ×104 = 細(xi)胞數/ml懸(xuan)液

細胞凍存(cun)

1. 重復“細(xi)胞傳代(dai)”中的(de)(de)消化(hua)(hua)細(xi)胞的(de)(de)步驟(zou)，將細(xi)胞消化(hua)(hua)下(xia)來，轉移(yi)至(zhi)離心管800轉/min，5分鐘離心，棄(qi)液

2. 沉淀中加入凍(dong)存(cun)液，輕吹制成懸液

3. 分轉到凍存(cun)管內，每個(ge)1~1.5ml

4. 將凍(dong)存管口(kou)封(feng)嚴

5.貼(tie)上標簽，注明凍存時間、細胞種類及代數

6. 按下列順序降溫，并zui終凍(dong)存(cun)：室溫→ 4℃ 20min → -20℃ 30min →-70℃凍(dong)存(cun)

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慧穎生物關于出售的 污染問題，請在收到產品48小時內，給我們提出真實的實驗結果；細胞活性問題，請在收到產品7天內給我們提出真實的實驗結果，我們調查核實后予免費調換，不收取任何費用。

需(xu)要(yao)客戶提供細胞(bao)培(pei)養說明、細胞(bao)操(cao)作處理方法、細胞(bao)質(zhi)量問題反饋(kui)表;如果搞不(bu)清原因的，或(huo)者客戶直(zhi)接愿意(yi)支付購買價半(ban)價的費用直(zhi)接復蘇(su)。

如(ru)用戶(hu)收(shou)到細胞(bao)(bao)8-30天(tian)之內，因(yin)處理(li)不當造成細胞(bao)(bao)死(si)亡或污染，我公(gong)司將以五折(zhe)優惠價重新提供一株原(yuan)細胞(bao)(bao)。

慧穎生(sheng)物(wu)科技(ji)（上海(hai)）有(you)限公司位于嘉定(ding)區南翔(xiang)眾仁路399號運通星財(cai)富(fu)廣場，由海(hai)外華僑和多名中科院博士組成的技(ji)術團隊，主(zhu)要致力于免疫學(xue)(xue)(xue)、分(fen)子生(sheng)物(wu)學(xue)(xue)(xue)、病(bing)(bing)理、病(bing)(bing)理分(fen)子生(sheng)物(wu)、代謝(xie)組學(xue)(xue)(xue)、生(sheng)物(wu)化學(xue)(xue)(xue)技(ji)術在科研(yan)中的應用與推廣，專業從事相關產(chan)品的引進(jin)、服務和研(yan)發(fa)。通過高度細(xi)分(fen)、*的服務平臺，向廣大(da)客戶提(ti)供醫(yi)學(xue)(xue)(xue)科研(yan)樣本(ben)檢測、合(he)作研(yan)究、開發(fa)及生(sheng)產(chan)。

主推產品一：ELISA試劑盒

1、專業的ELISA試(shi)劑盒生產(chan)企業

2、*抗體(ti)----高效、靈(ling)敏、特異

3、規范包被操作----吸(xi)附(fu)均勻，吸(xi)附(fu)性好，空白值低，孔底透明度高

4、*的優化方案----重復性高，可靠性強

5、購買本公(gong)司的ELISA試劑(ji)盒，免費代測(ce)

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8、可檢測動物類型豐富：人(ren)、猴、大鼠、小(xiao)鼠、豚鼠、黃金(jin)地(di)鼠、兔、豬、犬(quan)、牛、綿羊、山羊、鵝、雞、蝦、河蟹(xie)、鱸魚、斑(ban)馬魚等

9、可(ke)檢測指標齊全：炎癥因(yin)子(zi)、血管生成素、動脈粥樣硬化因(yin)子(zi)、趨(qu)化因(yin)子(zi)、生長因(yin)子(zi)基(ji)質金屬蛋白酶、脂(zhi)肪(fang)因(yin)子(zi)等等

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 我們(men)的優(you)勢(shi)：*、完善、穩定的實(shi)驗(yan)體系，優(you)秀的科(ke)研工作者(zhe)，準確可靠的實(shi)驗(yan)結果。稟(bing)存用戶至上的原(yuan)則，竭誠為你服務。

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慧穎以每年5000瓶血清(qing)出(chu)庫到全(quan)國(guo)各地重(zhong)點(dian)實(shi)驗室(shi)、高校、醫(yi)院(yuan)、制藥單位(wei)等，目(mu)前(qian)成(cheng)為全(quan)國(guo)幾(ji)大動(dong)物(wu)血清(qing)供(gong)應商之一(yi)。

主推產品三：細胞株細胞系

400多(duo)種類細(xi)(xi)(xi)胞(bao)株細(xi)(xi)(xi)胞(bao)系，20多(duo)株耐藥株，公司擁(yong)有完善的(de)細(xi)(xi)(xi)胞(bao)庫(ku)管(guan)理體系，80%細(xi)(xi)(xi)胞(bao)技術人(ren)員為碩(shuo)士學歷，二十多(duo)年的(de)細(xi)(xi)(xi)胞(bao)培養經驗，所(suo)售細(xi)(xi)(xi)胞(bao)背景清晰、傳代次數少(shao)、*、售后一對(dui)一服務，跟蹤及時(shi)到(dao)位。

熱賣(mai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)：DC2.4細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RAW264.7細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、COV434細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HO-8910細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A2780細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SK-OV-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCAR-3細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、OVCA433細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEK-293-6e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、FRO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HEPG2.2.15細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HL-7702細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、L-02細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M-ICC12細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、143B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HOS細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M4e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、M2e細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU686細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、TU212細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW48細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、SW480細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HT-22細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、B16-F10細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HK-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、C666-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、IOSE80細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HNE1/DDP細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、vero e6細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A375細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hs294T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HSC-LX-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LOVO細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MKN-28細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、RL952細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、A549細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、CACO2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、EBC-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、H1993細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep-2細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCI-H441細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、LS174T細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、NCCIT細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、capan-1細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、Hep3B細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、HUVEC細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、MC38細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)等。

另有(you)：我司代購美(mei)國SANTA CURZ、ABCAM、CST、密理(li)博抗(kang)體，全國格，！